| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
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Purity: ≥98%
| ln Vitro |
1. Components of TMRM working solution 1.1 Components of stock solution To make a 5 mM stock solution, take 1 milligram of TMRM and add 525 uL of DMSO. 1.2 Components of TMRM working solution To create a 1–20 μM working solution, use PBS stock solution or serum-free cell culture medium. Note: Please modify the TMRM working fluid concentration based on the current circumstances. 2. Suspension cells (6-well plate) 2.1 Cell staining a. After centrifuging for three to five minutes at 1000 g at 4 °C, remove the supernatant. Use PBS to wash twice, for five minutes each time. The density of cells is 1×106/mL. After adding 1 mL of the working solution, give it five to thirty minutes. Discard the supernatant after centrifuging at 400 g for three to four minutes at 4°C. Use PBS to wash twice, for five minutes each time. Re-suspend cells in PBS or serum-free culture media. observation using flow cytometry or fluorescence microscopy. 2.2 Integration with the cell wall a. On sterile slides, cultivate adherent cells. Aspirate extra culture medium after removing the coverslip from the medium. After adding 100 μL of working solution and giving it a little shake to cover all the cells, work for another 30 to 60 minutes. Wash your heart twice, for five minutes each time. observation using flow cytometry or fluorescence microscopy. Note: Prior to staining, cells must be resuspended if flow cytometry is being used for detection.
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| References |
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| Molecular Formula |
C25H25CLN2O7
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|---|---|
| Molecular Weight |
500.9282
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| Exact Mass |
500.135
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| CAS # |
115532-50-8
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| Related CAS # |
TMRM;115532-49-5
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| PubChem CID |
11755725
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| Appearance |
Green to dark green solid powder
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| Melting Point |
274–276℃
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| LogP |
4.303
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
35
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| Complexity |
868
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
PFYWPQMAWCYNGW-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C25H25N2O3.ClHO4/c1-26(2)16-10-12-20-22(14-16)30-23-15-17(27(3)4)11-13-21(23)24(20)18-8-6-7-9-19(18)25(28)29-5;2-1(3,4)5/h6-15H,1-5H3;(H,2,3,4,5)/q+1;/p-1
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| Chemical Name |
[6-(dimethylamino)-9-(2-methoxycarbonylphenyl)xanthen-3-ylidene]-dimethylazanium;perchlorate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~41.67 mg/mL (~83.19 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9963 mL | 9.9814 mL | 19.9629 mL | |
| 5 mM | 0.3993 mL | 1.9963 mL | 3.9926 mL | |
| 10 mM | 0.1996 mL | 0.9981 mL | 1.9963 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT02650154 | Completed | Trauma | The Hospital for Sick Children | 2013-08 | ||
| NCT03415503 | Completed | Drug: Medox® Anthocyanin capsules | Dyslipidemias | Sun Yat-sen University | 2018-10-01 | Phase 3 |
| NCT05071391 | Completed | Procedure: Roux-en-Y gastric bypass | Obesity | Milagros Rocha Barajas | 2017-01-01 |
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