| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
TMB-PS does not target a specific biological receptor but is used as a substrate for horseradish peroxidase (HRP) and other peroxidases. In the presence of HRP and hydrogen peroxide, TMB-PS is oxidized to a colored product that can be detected spectrophotometrically. The compound is widely used in clinical diagnostics, immunology, and biochemical research to detect and quantify antigens, antibodies, or enzymatic activity with high sensitivity and reproducibility.
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| ln Vitro |
In vitro, TMB-PS is a chromogenic substrate used in ELISAs and other peroxidase-based detection methods. It is soluble in water and can be used in a variety of pH environments. The compound is oxidized by HRP in the presence of hydrogen peroxide to produce a colored product. The intensity of the color is proportional to the amount of enzyme or target analyte present in the sample.
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| ln Vivo |
In vivo, TMB-PS is not typically used as a therapeutic agent. However, the detection methods that use TMB-PS can be applied to in vivo samples (e.g., serum, plasma) for diagnostic purposes. The compound itself is not administered to animals but is used in ex vivo assays to measure biomarkers in biological samples.
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| Enzyme Assay |
For in vitro ELISA protocols, TMB-PS is used as the substrate in the final detection step. After the antigen-antibody reaction and HRP-conjugated secondary antibody incubation, TMB-PS solution (typically 0.1-1 mM in appropriate buffer) is added to the wells. The plate is incubated at room temperature for 5-30 minutes until color development is observed. The reaction is stopped by adding an acid solution (e.g., H₂SO₄), and the absorbance is measured at 450 nm or 650 nm.
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| Cell Assay |
For in vitro cell-based assays, TMB-PS can be used to detect cellular proteins or secreted factors in cell culture supernatants using ELISA. Cells are cultured and treated with various stimuli. Supernatants are collected and analyzed using ELISA kits that utilize TMB-PS as the chromogenic substrate. The compound can also be used in dot blot or Western blot assays with HRP-conjugated detection antibodies.
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| Animal Protocol |
For in vivo animal experiments, TMB-PS is not administered to animals. However, blood or tissue samples collected from animals can be analyzed using TMB-PS-based ELISA to measure biomarkers such as cytokines, hormones, or antibodies. The compound enables sensitive and quantitative detection of these analytes in research and diagnostic applications.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for TMB-PS are not applicable, as it is not used as a therapeutic agent. The compound is a chromogenic substrate used in in vitro diagnostic assays. Its stability in solution and its reactivity with peroxidases are the primary considerations for its use. The compound should be stored protected from light and at appropriate temperatures to maintain activity.
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| Toxicity/Toxicokinetics |
Toxicological data for TMB-PS are limited. As a chromogenic substrate, it is classified as a research-grade reagent and is not for human therapeutic use. Standard laboratory safety practices should be followed when handling the compound. It may cause irritation upon contact with skin or eyes. The compound is not intended for in vivo administration.
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| Additional Infomation |
TMB-PS is a research-use only compound and has not been approved for clinical applications. It is also known as TMBZ-PS and N-(3-sulfopropyl)-3,3′,5,5′-tetramethylbenzidine sodium salt. Its molecular weight is 384.47 (sodium salt), and its formula is C₁₉H₂₅N₂NaO₃S. The compound is widely used in clinical diagnostics, immunology, and biochemical research and is available from various research chemical suppliers.
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| Molecular Formula |
C19H26N2O3S
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|---|---|
| Molecular Weight |
362.4863
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| Exact Mass |
362.166
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| CAS # |
102062-36-2
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| PubChem CID |
21972361
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Melting Point |
230-240ºC
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| Index of Refraction |
1.609
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| LogP |
2.52
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
25
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| Complexity |
495
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)NCCCS(=O)(=O)O)C
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| InChi Key |
GZNAFYJQMXPVSY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C19H26N2O3S/c1-12-8-16(9-13(2)18(12)20)17-10-14(3)19(15(4)11-17)21-6-5-7-25(22,23)24/h8-11,21H,5-7,20H2,1-4H3,(H,22,23,24)
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| Chemical Name |
3-[4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylanilino]propane-1-sulfonic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 35 mg/mL (~96.55 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7587 mL | 13.7935 mL | 27.5870 mL | |
| 5 mM | 0.5517 mL | 2.7587 mL | 5.5174 mL | |
| 10 mM | 0.2759 mL | 1.3793 mL | 2.7587 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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