| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg |
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| 1g | |||
| Other Sizes |
| Targets |
TMB monosulfate targets horseradish peroxidase (HRP) for chromogenic detection. As a substrate for HRP, it is oxidized in the presence of hydrogen peroxide to produce a blue-colored product. The compound also reacts with reactive oxygen and nitrogen species (ROS/RNS) including hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide. It is a chromogenic substrate used in immunohistochemistry and ELISA. TMB monosulfate is non-toxic and non-mutagenic.
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| ln Vitro |
In vitro, TMB monosulfate is a chromogenic substrate for HRP-based detection systems. It is used in staining procedures in immunohistochemistry and as a visualizing reagent in ELISA. The compound reacts with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. It is a non-toxic, non-mutagenic colorless dye. Its chromogenic properties have been characterized in various in vitro assays.
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| ln Vivo |
In vivo, TMB monosulfate is used as a chromogenic substrate in research applications. Its utility in immunohistochemistry and ELISA has been established. The compound is non-toxic and non-mutagenic. It can function as a photoacoustic probe for reactive oxygen and nitrogen species. Further in vivo studies have evaluated its potential as a diagnostic probe.
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| Enzyme Assay |
TMB monosulfate chromogenic assays involve measuring color development in HRP-based detection systems. The compound is incubated with HRP and hydrogen peroxide, and absorbance is measured at appropriate wavelengths (typically 450 nm or 650 nm). For ROS/RNS detection, the compound is incubated with reactive species and color development is monitored. Assays are performed in appropriate buffer systems with positive controls such as known HRP substrates.
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| Cell Assay |
In vitro cell-based assays for TMB monosulfate are limited as the compound is primarily a detection reagent rather than a therapeutic compound. The compound is used in ELISA and immunohistochemistry applications. For ROS/RNS detection, cells are treated with stimuli to generate reactive species, and TMB monosulfate is added as a probe. Color development is measured. Cell viability is assessed by standard assays. Experiments are performed with appropriate positive and negative controls.
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| Animal Protocol |
TMB monosulfate in vivo studies are limited as the compound is primarily a laboratory reagent. Its utility in research applications has been established. The compound is non-toxic and non-mutagenic. It is used in diagnostic and research applications. Studies are conducted in accordance with institutional guidelines for reagent use.
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| ADME/Pharmacokinetics |
TMB monosulfate (MW 338.42 g/mol, C16H22N2O4S) is a chromogenic substrate. It is also known as BM blue monosulfate. The compound is a white to off-white solid. It is soluble in DMSO and other appropriate solvents. It is stable as a powder at -20°C for up to 3 years and at 4°C for 2 years; in solvent, it is stable at -80°C for 6 months and at -20°C for 1 month. TMB monosulfate is a non-toxic, non-mutagenic dye.
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| Toxicity/Toxicokinetics |
TMB monosulfate is generally considered safe as a laboratory reagent. The compound is non-toxic and non-mutagenic. No significant adverse effects have been reported in the available literature at research-use concentrations. The compound is intended for research use only. Standard laboratory safety practices should be employed when handling this compound. Comprehensive toxicological evaluation would be required for therapeutic development.
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| Additional Infomation |
TMB monosulfate (BM blue monosulfate) is a chromogenic substrate used in immunohistochemistry and ELISA. It is a non-toxic, non-mutagenic colorless dye used in HRP-based enzyme detection systems. TMB monosulfate functions as a photoacoustic probe for reactive oxygen and nitrogen species. Its molecular formula is C16H22N2O4S with a molecular weight of 338.42 g/mol. All applications are limited to non-human research use.
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| Molecular Formula |
C16H22N2O4S
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| Molecular Weight |
338.4219
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| Exact Mass |
338.13
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| CAS # |
54827-18-8
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| Related CAS # |
TMB;54827-17-7;TMB dihydrochloride;64285-73-0
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| PubChem CID |
67612326
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
23
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| Complexity |
308
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
VJSXOBXXVSDFKR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H20N2.H2O4S/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;1-5(2,3)4/h5-8H,17-18H2,1-4H3;(H2,1,2,3,4)
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| Chemical Name |
4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline;sulfuric acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 28 mg/mL (~82.74 mM)
H2O : ~2.78 mg/mL (~8.21 mM) |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9549 mL | 14.7745 mL | 29.5491 mL | |
| 5 mM | 0.5910 mL | 2.9549 mL | 5.9098 mL | |
| 10 mM | 0.2955 mL | 1.4775 mL | 2.9549 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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