| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 100mg | |||
| Other Sizes |
| Targets |
This compound is a multifunctional bioconjugation reagent rather than a drug with a specific biological target. The azide group enables copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-tagged molecules. The biotin group provides high-affinity binding to streptavidin (Kd ~10⁻¹⁵ M). The TAMRA fluorophore allows for fluorescent detection (Abs/Em ~545/565 nm).
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|---|---|
| ln Vitro |
Two distinct ligands, one for the E3 ubiquitin ligase and the other for the target protein, are present in PROTAC and are joined by a linker. PROTAC selectively degrades target proteins by means of the intracellular ubiquitin-proteasome system [1].
The compound does not possess direct biological activity as it is a linker. Its in vitro utility lies in its ability to form stable conjugates via click chemistry, enabling the labeling and detection of biomolecules. The hydrophilic PEG spacer increases aqueous solubility. It is used as a linker and spacer to add a PEG moiety to proteins, peptides, oligonucleotides, small molecules, and nanoparticles. |
| ln Vivo |
The compound is a chemical reagent and does not have direct in vivo biological activity. Its utility in vivo is as a component of bioconjugates, where it can be used for imaging and affinity purification applications. The biotin group enables streptavidin-based capture and enrichment, while the TAMRA fluorophore allows for fluorescent detection in biological samples.
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| Enzyme Assay |
As a chemical linker, the compound is not used in biological enzyme assays. Its utility lies in chemical conjugation reactions. The azide group reacts with alkynes via CuAAC click chemistry. The reaction is typically carried out in the presence of a copper catalyst and a reducing agent. The biotin group enables affinity purification using streptavidin beads. Conjugation efficiency is monitored by HPLC, LC-MS, or gel electrophoresis.
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| Cell Assay |
For in vitro cell-based assays using conjugates synthesized with this linker, target cells are treated with the conjugate at various concentrations. The biological activity depends on the conjugated molecule rather than the linker itself. The TAMRA fluorophore allows for visualization by fluorescence microscopy or flow cytometry. The biotin group enables detection using streptavidin-conjugated probes.
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| ADME/Pharmacokinetics |
TAMRA-Azide-PEG-Biotin (CAS 1797415-74-7) is a solid powder soluble in DMSO, water, DMF, and DCM. It should be stored in a dry, dark place at 0-4°C for short-term or -20°C for long-term storage. The molecular formula is C57H79N11O14S and molecular weight is 1174.38.
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| Toxicity/Toxicokinetics |
As a chemical linker, the compound is not a pharmacologically active compound and does not have direct toxicity. Toxicity would be associated with the final conjugates synthesized using this linker. The PEG component is generally considered biocompatible and non-toxic. No specific toxicity data are provided in the available literature.
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| References | |
| Additional Infomation |
This compound is a sophisticated, multifunctional bioconjugation reagent. It is used in affinity purification, protein enrichment, and click chemistry workflows. It is for research use only and is not for human or clinical use. No approved therapeutic status is reported.
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| Molecular Formula |
C57H79N11O14S
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|---|---|
| Molecular Weight |
1174.36747288704
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| Exact Mass |
2348.109
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| CAS # |
1797415-74-7
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| PubChem CID |
146026259
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| Appearance |
Purple to purplish red solid powder
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| Hydrogen Bond Donor Count |
10
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| Hydrogen Bond Acceptor Count |
36
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| Rotatable Bond Count |
76
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| Heavy Atom Count |
166
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| Complexity |
4530
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| Defined Atom Stereocenter Count |
0
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| SMILES |
S1C[C@H]2[C@@H]([C@@H]1CCCCC(NCCOCCOCCOCCNC(CCN(C(CCNC(C1C=CC(=C(C(=O)[O-])C=1)C1C3C=CC(=CC=3OC3=C/C(/C=CC=13)=[N+](\C)/C)N(C)C)=O)=O)CCOCCOCCOCCN=[N+]=[N-])=O)=O)NC(N2)=O
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| InChi Key |
OLNZJSRKGRJBHG-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/2C57H79N11O14S/c1-66(2)40-10-13-44-48(36-40)82-49-37-41(67(3)4)11-14-45(49)53(44)46-35-39(9-12-43(46)56(73)74)54(72)61-17-15-52(71)68(22-26-79-30-34-81-33-29-78-25-20-62-65-58)21-16-51(70)60-19-24-77-28-32-80-31-27-76-23-18-59-50(69)8-6-5-7-42-38-47-55(83-42)64-57(75)63-47;1-66(2)40-10-13-44-48(36-40)82-49-37-41(67(3)4)11-14-45(49)53(44)43-12-9-39(35-46(43)56(73)74)54(72)61-17-15-52(71)68(22-26-79-30-34-81-33-29-78-25-20-62-65-58)21-16-51(70)60-19-24-77-28-32-80-31-27-76-23-18-59-50(69)8-6-5-7-42-38-47-55(83-42)64-57(75)63-47/h2*9-14,35-37,42,47,55H,5-8,15-34,38H2,1-4H3,(H5-,59,60,61,63,64,69,70,72,73,74,75)
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| Chemical Name |
4-[[3-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethyl-[3-oxo-3-[2-[2-[2-[2-[5-(2-oxo-1,3,3a,5,6,6a-hexahydrothieno[2,3-d]imidazol-5-yl)pentanoylamino]ethoxy]ethoxy]ethoxy]ethylamino]propyl]amino]-3-oxopropyl]carbamoyl]-2-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]benzoate;5-[[3-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethyl-[3-oxo-3-[2-[2-[2-[2-[5-(2-oxo-1,3,3a,5,6,6a-hexahydrothieno[2,3-d]imidazol-5-yl)pentanoylamino]ethoxy]ethoxy]ethoxy]ethylamino]propyl]amino]-3-oxopropyl]carbamoyl]-2-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]benzoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.8515 mL | 4.2576 mL | 8.5152 mL | |
| 5 mM | 0.1703 mL | 0.8515 mL | 1.7030 mL | |
| 10 mM | 0.0852 mL | 0.4258 mL | 0.8515 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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