| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg | |||
| 250mg | |||
| 500mg | |||
| Other Sizes |
Purity: ≥98%
| Targets |
Sulfo-NHS-Biotin targets primary amines on proteins, peptides, and other biomolecules. As an NHS ester, it reacts with primary amines (such as the epsilon-amino group of lysine residues) to form stable amide bonds. This reaction is highly specific and efficient, making it a useful tool for biotinylation. The biotin moiety then binds to streptavidin or avidin with high affinity, enabling detection, purification, or immobilization of the labeled molecule. Its membrane impermeability makes it particularly useful for labeling cell surface proteins.
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| ln Vitro |
In vitro, sulfo-NHS-biotin is used for biotinylating antibodies or proteins. The biotinylated proteins can then be used for protein detection or immobilization when used in conjunction with streptavidin and immobilized streptavidin. It is particularly useful for targeting cell surface proteins in Streptavidin-Biotin assays. It is a water-soluble biotinylation reagent typically coupled to primary amines in the pH range 6.5-8.5. It is also a fluorogenic reagent for specific C-terminal labeling of peptides and proteins by carboxypeptidase Y catalyzed transpeptidation reactions.
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| ln Vivo |
Sulfo-NHS-Biotin is not used in vivo as a therapeutic agent; it is a research reagent used for biotinylation and protein labeling. It is used to biotinylate proteins, which can then be used for detection, purification, or immobilization in various assays. Its membrane impermeability makes it useful for labeling cell surface proteins in vivo or in tissue sections.
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| Enzyme Assay |
Sulfo-NHS-Biotin is a chemical reagent that reacts with primary amines. In a typical assay, a protein or peptide containing primary amines is incubated with sulfo-NHS-biotin in an aqueous buffer at pH 7-9. The reaction results in the biotinylation of the primary amines, which can be detected by streptavidin-based methods such as Western blotting, ELISA, or flow cytometry.
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| Cell Assay |
Sulfo-NHS-Biotin is dissolved in DMSO or water and applied to cells or proteins at concentrations ranging from 1-100 µM. It is used to label cell surface proteins in live cells, as it is membrane-impermeable. After incubation, the cells are washed, and the biotinylated proteins are detected using streptavidin-conjugated probes.
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| ADME/Pharmacokinetics |
Sulfo-NHS-Biotin has a molecular weight of 443.43 g/mol and a molecular formula of C14H18N3NaO8S2. It is soluble in water (10 mg/mL) and DMSO (≥50 mg/mL). The compound is typically stored as a powder at -20°C for long-term stability. Sulfo-NHS-Biotin is also known as sulfosuccinimidyl biotin or Biotin-NHS.
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| Toxicity/Toxicokinetics |
Sulfo-NHS-Biotin is considered to have low toxicity for research use. It is a chemical reagent that should be handled with appropriate safety precautions. It may cause skin and eye irritation. Inhalation and ingestion should be avoided. Proper personal protective equipment (PPE) should be used when handling the compound.
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| References |
: Pintado CO, Moreno A, Barbancho M, Pérez de la Lastra JM, Llanes D. The beta chain of the GPIIb molecule on ruminant leukocytes and platelets is not labelled by the sulfo-NHS-biotin method. Vox Sang. 1995;69(3):248-9. PubMed PMID: 8578739.
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| Additional Infomation |
Sulfo-NHS-Biotin is a water-soluble, amine-reactive biotinylation reagent. It is a membrane-impermeable biotinylating reagent used in Biotin-Streptavidin binding reactions. Sulfo-NHS-Biotin is ideal for biotinylating antibodies or proteins that can then be used for protein detection or immobilization when used in conjunction with streptavidin and immobilized streptavidin. It is particularly useful for targeting cell surface proteins in Streptavidin-Biotin assays.
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| Molecular Formula |
C14H18N3NAO8S2
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|---|---|
| Molecular Weight |
405.44656
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| Exact Mass |
443.043
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| CAS # |
119616-38-5
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| Related CAS # |
119616-38-5;194041-65-1;
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| PubChem CID |
23667738
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.64g/cm3
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| Index of Refraction |
1.658
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| LogP |
0.519
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
28
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| Complexity |
771
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| Defined Atom Stereocenter Count |
2
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| SMILES |
O=C1NC2CSC(CCCCC(N3C(=O)CC(S(=O)(O)=O)C3=O)=O)C2N1
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| InChi Key |
DREOJRVDBCALEG-WCYHLEDJSA-M
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| InChi Code |
InChI=1S/C14H19N3O8S2.Na/c18-10-5-9(27(22,23)24)13(20)17(10)25-11(19)4-2-1-3-8-12-7(6-26-8)15-14(21)16-12/h7-9,12H,1-6H2,(H2,15,16,21)(H,22,23,24)/q+1/p-1/t7-,8-,9?,12-/m0./s1
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| Chemical Name |
Biotin 3-sulfo-N-hydroxysuccinimide ester sodium salt
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| Synonyms |
Biotin 3-sulfo-N-hydroxysuccinimide ester sodium salt Sulfosuccinimidobiotin Sulfo NHS BiotinSulfosuccinimidyl biotin N-hydroxysulfosuccinimido biotin
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4664 mL | 12.3320 mL | 24.6640 mL | |
| 5 mM | 0.4933 mL | 2.4664 mL | 4.9328 mL | |
| 10 mM | 0.2466 mL | 1.2332 mL | 2.4664 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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