| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 500mg |
| Targets |
Sulfo-NHS-Acetate targets primary amines on proteins, peptides, and other biomolecules. As an NHS ester, it reacts with primary amines (such as the epsilon-amino group of lysine residues) to form stable amide bonds. This reaction is highly specific and efficient, making it a useful tool for protein modification. It is also used as a PROTAC linker, targeting proteins for degradation via the ubiquitin-proteasome system.
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|---|---|
| ln Vitro |
One ligand is for an E3 ubiquitin ligase, and the other is for the target protein; these two ligands are joined by a linker to form PROTACs. The intracellular ubiquitin-proteasome system is utilized by PROTACs to specifically destroy target proteins[1].
Sulfo-NHS-Acetate is a water-soluble, amine-reactive reagent used for precision protein modification and bioconjugation. It is used to prevent polymerization when performing protein crosslinking reactions. It is also used when conjugating peptides to carrier proteins for immunogen production. As a PROTAC linker, it is used in the synthesis of PROTACs to target specific proteins for degradation. |
| ln Vivo |
Sulfo-NHS-Acetate is not used in vivo as a therapeutic agent; it is a research reagent used for protein modification and bioconjugation. It is used to acetylate primary amines on proteins, which can be used to block reactive sites or to introduce a small, neutral modification. It is also used as a PROTAC linker in the synthesis of PROTACs for targeted protein degradation.
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| Enzyme Assay |
Sulfo-NHS-Acetate is a chemical reagent that reacts with primary amines. In a typical assay, a protein or peptide containing primary amines is incubated with sulfo-NHS-acetate in an aqueous buffer at pH 7-9. The reaction results in the acetylation of the primary amines, which can be monitored by mass spectrometry or by measuring the change in charge or hydrophobicity.
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| Cell Assay |
For in vitro cell-based assays, sulfo-NHS-acetate is dissolved in DMSO or aqueous buffers and applied to cells at concentrations ranging from 1-100 µM. It is used to modify cell surface proteins or to deliver PROTACs into cells for targeted protein degradation.
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| ADME/Pharmacokinetics |
Sulfo-NHS-Acetate has a molecular weight of 237.19 g/mol (free acid) or 259.17 g/mol (sodium salt) and a molecular formula of C6H7NO7S (free acid) or C6H6NO7S•Na (sodium salt). It is a water-soluble compound. The compound is typically stored as a powder at -20°C for long-term stability. Sulfo-NHS-Acetate is also known as sulfosuccinimidyl acetate or 1-acetoxy-2,5-dioxopyrrolidine-3-sulfonic acid.
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| Toxicity/Toxicokinetics |
Sulfo-NHS-Acetate is considered to have low toxicity for research use. It is a chemical reagent that should be handled with appropriate safety precautions. It may cause skin and eye irritation. Inhalation and ingestion should be avoided. Proper personal protective equipment (PPE) should be used when handling the compound.
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| References | |
| Additional Infomation |
Sulfo-NHS-Acetate is a water-soluble, amine-reactive chemical reagent belonging to the N-hydroxysuccinimide (NHS) ester class. It is a PROTAC linker of the alkyl chain class, utilized to prepare PROTAC protein degraders. Sulfo-NHS-Acetate is typically used to prevent polymerization when performing protein crosslinking reactions and when conjugating peptides to carrier proteins for immunogen production. It is a water-soluble amine-reactive reagent for precision protein modification and bioconjugation.
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| Molecular Formula |
C6H7NO7S
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|---|---|
| Molecular Weight |
237.18728
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| Exact Mass |
236.994
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| CAS # |
152305-87-8
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| PubChem CID |
127857
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| Appearance |
White to off-white solid powder
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| Density |
1.8g/cm3
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| Index of Refraction |
1.583
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| LogP |
-1.2
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
15
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| Complexity |
419
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
MDUQWFYJHRLNRN-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H7NO7S/c1-3(8)14-7-5(9)2-4(6(7)10)15(11,12)13/h4H,2H2,1H3,(H,11,12,13)
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| Chemical Name |
1-acetyloxy-2,5-dioxopyrrolidine-3-sulfonic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~527.00 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.2160 mL | 21.0801 mL | 42.1603 mL | |
| 5 mM | 0.8432 mL | 4.2160 mL | 8.4321 mL | |
| 10 mM | 0.4216 mL | 2.1080 mL | 4.2160 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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