| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Sulfiram is a weak aldehyde dehydrogenase (ALDH) inhibitor. It also functions as an ectoparasiticide targeting Sarcoptes scabiei, the mite that causes scabies. Sulfiram has been investigated for its potential anticancer properties and as an adjunct treatment in certain cancers due to its ability to chelate copper ions, which are involved in tumor progression.
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| ln Vitro |
Sulfiram exhibits antifungal and antibacterial activities. It inhibits aldehyde dehydrogenase activity, although it is considered a weak inhibitor. As an ectoparasiticide, it is effective against Sarcoptes scabiei, the scabies mite. Its ability to chelate metal ions, particularly copper, contributes to its potential anticancer properties. Specific IC50 values against ALDH or parasites are not extensively detailed.
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| ln Vivo |
Sulfiram is applied topically to treat scabies in both humans and domestic animals. In vivo efficacy has been demonstrated in patients with scabies infestations. As an ALDH inhibitor, it has been studied for its potential in alcohol dependence and as an adjunct in cancer therapy. Specific animal model data are not extensively detailed in standard reference sources.
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| Enzyme Assay |
Sulfiram's inhibition of aldehyde dehydrogenase (ALDH) can be assessed using in vitro enzyme activity assays. ALDH enzyme is incubated with its substrate (e.g., acetaldehyde) and NAD+ in the presence of varying concentrations of sulfiram. The production of NADH is measured spectrophotometrically at 340 nm to determine enzyme activity. The IC50 value for ALDH inhibition is calculated from dose-response curves. For studying its anti-parasitic activity, in vitro assays using Sarcoptes scabiei mites can be performed.
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| Cell Assay |
Sulfiram's anti-parasitic activity can be evaluated in vitro using Sarcoptes scabiei mites collected from infected animals or humans. Mites are incubated in culture media containing various concentrations of sulfiram, and mite survival is monitored over time. The concentration required to kill 50% of the mites (LC50) is determined. For studying its anticancer properties, cancer cell lines can be treated with sulfiram and cell viability assessed using MTT or CellTiter-Glo assays. ALDH activity in treated cells can be measured using ALDH activity assays.
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| Animal Protocol |
Sulfiram is typically applied topically for the treatment of scabies. In animal models of scabies, such as pigs or dogs naturally infested with Sarcoptes scabiei, sulfiram is applied topically as a lotion or ointment. The treatment is usually repeated weekly. Efficacy is assessed by skin scrapings to count live mites and by monitoring clinical signs such as pruritus and skin lesions. For other indications (e.g., cancer), oral or intraperitoneal administration in mouse models may be used.
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| ADME/Pharmacokinetics |
Sulfiram has a molecular weight of 264.47 and is typically a viscous, light yellow liquid. Its purity is typically ≥96%. Specific pharmacokinetic parameters such as absorption, distribution, metabolism, and excretion are not extensively detailed in standard reference sources. Topical application suggests limited systemic absorption.
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| Toxicity/Toxicokinetics |
Sulfiram is generally well-tolerated when applied topically for the treatment of scabies. It is a weak ALDH inhibitor, and its toxicity profile is less severe than that of disulfiram, which is a more potent ALDH inhibitor used for alcohol aversion therapy. Specific LD50 values and organ-specific toxicity data are not extensively detailed in standard reference sources.
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| References | |
| Additional Infomation |
Sulfiram are organic sulfur compounds.
Sulfiram (CAS# 95-05-6) is also known as monosulfiram. Its primary application has been targeted at Sarcoptes scabiei infestations in both humans and domestic animals. Sulfiram has also been investigated for its potential anticancer properties and as an adjunct treatment in certain cancers. Its ability to chelate copper ions is relevant to its potential in cancer research. |
| Molecular Formula |
C10H20N2S3
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|---|---|
| Molecular Weight |
264.46
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| Exact Mass |
264.079
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| CAS # |
95-05-6
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| PubChem CID |
7215
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| Appearance |
Light yellow to yellow viscous liquid
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| Density |
1.139g/cm3
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| Boiling Point |
329.1ºC at 760mmHg
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| Flash Point |
152.8ºC
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| Index of Refraction |
1.59
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| LogP |
2.973
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
15
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| Complexity |
189
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCN(CC)C(=S)SC(=S)N(CC)CC
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| InChi Key |
CTPKSRZFJSJGML-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H20N2S3/c1-5-11(6-2)9(13)15-10(14)12(7-3)8-4/h5-8H2,1-4H3
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| Chemical Name |
diethylcarbamothioyl N,N-diethylcarbamodithioate
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| Synonyms |
NSC-36731; NSC 36731; Sulfiram
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7813 mL | 18.9065 mL | 37.8129 mL | |
| 5 mM | 0.7563 mL | 3.7813 mL | 7.5626 mL | |
| 10 mM | 0.3781 mL | 1.8906 mL | 3.7813 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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