| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
The unlabeled parent compound, sulfamethazine, targets bacterial dihydropteroate synthase, inhibiting folic acid synthesis. Sulfamethazine-13C6 shares this mechanism. It is a 13C-labeled internal standard for LC-MS/MS quantification of sulfamethazine in biological and environmental samples.
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| ln Vitro |
In the process of developing new drugs, stable heavy isotopes of carbon, hydrogen, and other elements have been added to pharmacological molecules, mostly as quantitative tracers. Due to its potential effects on medication pharmacokinetics and metabolic properties, deuteration is a matter for worry [75].
Sulfamethazine inhibits bacterial growth by blocking folate synthesis. The 13C-labeled version is not used for biological activity assays but is used as an internal standard to correct for matrix effects and ion suppression in mass spectrometry, improving accuracy and precision of sulfamethazine quantification. |
| ln Vivo |
This compound is not a therapeutic agent but an analytical internal standard. In vivo, it would behave similarly to unlabeled sulfamethazine. It is used as a tracer in pharmacokinetic studies to quantify the parent drug without interference from endogenous compounds, providing accurate oral bioavailability and tissue distribution data.
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| Enzyme Assay |
For LC-MS/MS method development, a standard solution of Sulfamethazine-13C6 is spiked into biological or environmental samples (plasma, tissue homogenates, water, food) at a known concentration (e.g., 10-100 ng/mL). After sample preparation (protein precipitation, solid-phase extraction), the sample is analyzed by LC-MS/MS. The peak area ratio (analyte/IS) is used to back-calculate the concentration of unlabeled sulfamethazine.
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| Cell Assay |
For in vitro metabolism studies, hepatocytes or liver microsomes are incubated with unlabeled sulfamethazine (1-100 uM) and a fixed concentration of Sulfamethazine-13C6 (as internal standard). After incubation (0-60 min), reactions are terminated and samples are analyzed by LC-MS/MS to determine the amount of sulfamethazine remaining and to identify metabolites.
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| Animal Protocol |
For pharmacokinetic studies in animals (e.g., swine, cattle, rodents), unlabeled sulfamethazine is administered (oral, IV, IM). Blood samples are collected at various time points. A fixed amount of Sulfamethazine-13C6 (internal standard) is added to each sample prior to LC-MS/MS analysis. The concentration of sulfamethazine is determined by the peak area ratio (analyte/IS), and PK parameters are calculated.
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| ADME/Pharmacokinetics |
Sulfamethazine-13C6 has identical physicochemical properties and PK behavior to unlabeled sulfamethazine. It is not a separate drug entity but an analytical tool. It is stable in solution for extended periods when stored at -20degC. Storage: powder at -20degC for 3 years; in solvent at -80degC for 6 months.
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| Toxicity/Toxicokinetics |
Standard sulfonamide safety precautions apply. Sulfamethazine is generally well-tolerated in animals at therapeutic doses but may cause crystalluria, hypersensitivity reactions, and blood dyscrasias. The 13C-labeled form carries the same safety profile as the unlabeled drug. Use personal protective equipment when handling.
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| References | |
| Additional Infomation |
Sulfamethazine-13C6 is a research-grade stable isotope-labeled internal standard, not a therapeutic. It is used for regulatory and analytical applications, including residue monitoring in food (milk, meat, eggs) and environmental analysis. Unlabeled sulfamethazine is used in veterinary medicine. Molecular formula: C₆¹3C₆H14N4O2S; MW: 284.29. Purity: ≥98%. Unlabeled CAS: 57-68-1.
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| Molecular Formula |
C12H14N4O2S
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|---|---|
| Molecular Weight |
284.286089420319
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| Exact Mass |
284.103
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| CAS # |
77643-91-5
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| Related CAS # |
Sulfamethazine;57-68-1
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| PubChem CID |
71312513
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| Appearance |
White to off-white solid powder
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| LogP |
0.3
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
19
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| Complexity |
377
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC1=CC(=NC(=N1)NS(=O)(=O)[13C]2=[13CH][13CH]=[13C]([13CH]=[13CH]2)N)C
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| InChi Key |
ASWVTGNCAZCNNR-BULCFLCISA-N
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| InChi Code |
InChI=1S/C12H14N4O2S/c1-8-7-9(2)15-12(14-8)16-19(17,18)11-5-3-10(13)4-6-11/h3-7H,13H2,1-2H3,(H,14,15,16)/i3+1,4+1,5+1,6+1,10+1,11+1
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| Chemical Name |
4-amino-N-(4,6-dimethylpyrimidin-2-yl)(1,2,3,4,5,6-13C6)cyclohexa-1,3,5-triene-1-sulfonamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5175 mL | 17.5877 mL | 35.1753 mL | |
| 5 mM | 0.7035 mL | 3.5175 mL | 7.0351 mL | |
| 10 mM | 0.3518 mL | 1.7588 mL | 3.5175 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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