| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Sulfadimethoxine D4 targets the same enzymes as its unlabeled parent drug. Sulfadimethoxine is a sulfonamide antibiotic that inhibits bacterial synthesis of dihydrofolic acid by competing with para-aminobenzoic acid (PABA) for binding to dihydropteroate synthase, thereby preventing bacterial growth.
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|---|---|
| ln Vitro |
Sulfadimethoxine D4 is primarily used as an analytical internal standard rather than for its pharmacological activity. Sulfadimethoxine is a sulfonamide antibiotic used in the treatment of a variety of infections like respiratory, urinary tract, intestinal and soft tissue infections.
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| ln Vivo |
Sulfadimethoxine D4 is used in pharmacokinetic studies as an internal standard for the quantification of Sulfadimethoxine. Sulfadimethoxine is a sulfonamide antibiotic used to treat many infections. The deuterium-labeled compound can be co-administered with unlabeled drug to study absorption, distribution, metabolism, and excretion.
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| Enzyme Assay |
In vitro enzyme assays for Sulfadimethoxine D4 are typically performed as part of analytical method validation. The compound is used as an internal standard in LC-MS/MS assays for the quantification of Sulfadimethoxine. It is dissolved in an appropriate solvent and spiked into biological matrices at known concentrations. Calibration curves are generated by plotting the peak area ratio of Sulfadimethoxine to Sulfadimethoxine D4 against the concentration of Sulfadimethoxine.
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| Cell Assay |
For in vitro cell-based assays, Sulfadimethoxine D4 can be used in studies investigating Sulfadimethoxine's antibacterial effects, though its primary use is as an analytical internal standard. Bacterial cultures are treated with the compound, and bacterial growth is monitored. However, due to its role as an internal standard, it is more frequently used in the analytical quantification of the parent drug.
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| Animal Protocol |
In vivo animal studies with Sulfadimethoxine D4 are primarily pharmacokinetic. The compound is administered orally to rodents, and blood samples are collected at predetermined time points. Plasma concentrations of both labeled and unlabeled Sulfadimethoxine are analyzed by LC-MS/MS using Sulfadimethoxine D4 as the internal standard. Pharmacodynamic effects can be evaluated in infection models.
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| ADME/Pharmacokinetics |
Sulfadimethoxine D4 (CAS: 1020719-80-5) has a molecular weight of 314.30 g/mol and a molecular formula of C₁₂H₁₄D₄N₄O₄S. Appearance: Light yellow to brown solid powder. Melting Point: 192-195°C. Storage: Powder -20°C 3 years, 4°C 2 years; In solvent -80°C 6 months, -20°C 1 month.
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| Toxicity/Toxicokinetics |
As a deuterium-labeled analog of a sulfonamide antibiotic, Sulfadimethoxine D4 is expected to have a similar toxicity profile to Sulfadimethoxine, though toxicity studies are typically not performed on the labeled compound. Sulfonamides are generally well-tolerated but can cause allergic reactions. The compound is used in very small quantities as an analytical internal standard and does not pose significant toxicity risks under normal handling.
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| References | |
| Additional Infomation |
Sulfadimethoxine D4 is a deuterium-labeled form of Sulfadimethoxine (Sulphadimethoxine), a sulfonamide antibiotic. It is intended for use as an internal standard for the quantification of sulfadimethoxine by GC- or LC-MS. Sulfadimethoxine is used to treat a variety of infections. The compound is not intended for therapeutic use and is for research and analytical use only.
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| Molecular Formula |
C12H14D4N4O4S
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|---|---|
| Molecular Weight |
314.3
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| Exact Mass |
314.098
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| CAS # |
1020719-80-5
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| Related CAS # |
Sulfadimethoxine;122-11-2;Sulfadimethoxine sodium;1037-50-9;Sulfadimethoxine-d6;73068-02-7;Sulfadimethoxine-13C6;1334378-48-1
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| PubChem CID |
45040436
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| Appearance |
Light yellow to brown solid powder
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| Melting Point |
192-195°C
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| LogP |
1.6
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
21
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| Complexity |
420
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C1=C(C(=C(C(=C1N)[2H])[2H])S(=O)(=O)NC2=CC(=NC(=N2)OC)OC)[2H]
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| InChi Key |
ZZORFUFYDOWNEF-LNFUJOGGSA-N
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| InChi Code |
InChI=1S/C12H14N4O4S/c1-19-11-7-10(14-12(15-11)20-2)16-21(17,18)9-5-3-8(13)4-6-9/h3-7H,13H2,1-2H3,(H,14,15,16)/i3D,4D,5D,6D
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| Chemical Name |
4-amino-2,3,5,6-tetradeuterio-N-(2,6-dimethoxypyrimidin-4-yl)benzenesulfonamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1817 mL | 15.9084 mL | 31.8167 mL | |
| 5 mM | 0.6363 mL | 3.1817 mL | 6.3633 mL | |
| 10 mM | 0.3182 mL | 1.5908 mL | 3.1817 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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