| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| Targets |
Endogenous metabolite; sucrase-isomaltase (EC 3.2.1.48); GLUT5; GLUT2; SGLT1; insulin signaling pathway. Sucrose is hydrolyzed by sucrase-isomaltase to glucose and fructose in the small intestine. Glucose and fructose are then absorbed via SGLT1/GLUT2 and GLUT5 transporters, respectively, and enter glycolysis and lipogenesis pathways.
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|---|---|
| ln Vitro |
As a labeled metabolite, Sucrose-d2 exhibits the same intrinsic biological activity as unlabeled sucrose. It is hydrolyzed by sucrase-isomaltase with Km values in the millimolar range. In vitro, it is used for metabolic tracing of sucrose digestion and absorption. The d2 label enables quantification of sucrose-derived glucose and fructose by LC-MS/MS.
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| ln Vivo |
In vivo, Sucrose-d2 is used for metabolic tracing of sucrose absorption and metabolism. Following oral administration, sucrose is hydrolyzed to glucose and fructose in the small intestine. The d2 label enables quantification of sucrose-derived glucose in blood and tissues. Sucrose supplementation is used in animal models of metabolic disease, obesity, and diabetes.
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| Enzyme Assay |
For sucrase-isomaltase assays, incubate the enzyme with Sucrose-d2 (0.1-10 mM) in assay buffer at 37°C. Measure glucose release by glucose oxidase/peroxidase assay or HPLC. Analyze products by LC-MS/MS to quantify d2 enrichment in glucose and fructose. For intestinal absorption studies, use everted gut sacs or Ussing chambers.
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| Cell Assay |
For cell culture studies, culture intestinal epithelial cells (Caco-2) with Sucrose-d2 (1-25 mM) for 1-24 hours. Extract metabolites and analyze by LC-MS/MS for d2 enrichment in glucose, fructose, and lactate. For insulin secretion studies, incubate pancreatic islets with the compound, and measure insulin release by ELISA.
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| Animal Protocol |
For in vivo metabolic tracing, mice or rats receive Sucrose-d2 (100-500 mg/kg) via oral gavage. Collect blood and tissues (liver, muscle, adipose) at 0-8 hours. Extract metabolites and analyze by LC-MS/MS to quantify d2 enrichment in glucose, fructose, and lactate.
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| Molecular Formula |
C12H20D2O11
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|---|---|
| Molecular Weight |
344.31
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| Appearance |
Typically exists as solid at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9044 mL | 14.5218 mL | 29.0436 mL | |
| 5 mM | 0.5809 mL | 2.9044 mL | 5.8087 mL | |
| 10 mM | 0.2904 mL | 1.4522 mL | 2.9044 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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