| Size | Price | Stock | Qty |
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| 5mg |
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| ln Vitro |
1. In silver nanoparticle (AgNP) green synthesis system: Stigmasterol glucoside (extracted from related biomass) acted as a capping and reducing agent in the AgNP synthesis using Plectranthus asirensis biomass; it promoted the formation of monodispersed AgNPs with a mean particle size of 22 nm (characterized by UV-Vis spectroscopy, XRD and TEM); the AgNPs synthesized with participation of Stigmasterol glucoside exhibited strong chemiluminescence (CL) activity in the luminol-H₂O₂ system[1]
2. In sequential injection-chemiluminescence (SI-CL) detection: Stigmasterol glucoside showed a specific CL response in the established SI-CL system; the CL intensity had a linear relationship with the concentration of Stigmasterol glucoside in the range of 0.05-10 μM, with a detection limit of 0.02 μM; the response was selective, and luteolin (co-existing compound) did not interfere with its detection[1] |
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| Enzyme Assay |
1. Sequential injection-chemiluminescence detection assay for Stigmasterol glucoside: First, construct the SI-CL system consisting of luminol solution, H₂O₂ solution, and the carrier buffer; prepare a series of standard solutions of Stigmasterol glucoside with concentrations ranging from 0.05 μM to 10 μM and the sample solution containing Stigmasterol glucoside; set the sequential injection program (including sample loading volume, reagent mixing ratio, and reaction time) to deliver the sample/standard solution, luminol and H₂O₂ into the reaction coil in sequence; detect the chemiluminescence intensity of the reaction system using a photomultiplier tube; establish a calibration curve based on the CL intensity and standard concentration, then quantify the content of Stigmasterol glucoside in the sample[1]
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| References |
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| Additional Infomation |
Stigmasterol-3-O-β-D-glucoside is a steroidal saponin with the structure (3β,22E)-stigmasterol-5,22-dien-3-ol linked to a β-D-glucopyranose residue at position 3 via a glycosidic bond. It was isolated from Symplocos lancifolia as a metabolite. It belongs to the phytosterol class of compounds and is a steroidal saponin, β-D-glucoside, and monosaccharide derivative. Its function is related to stigmasterol. It is derived from the hydride of stigmasterane. Stigmasterol glucoside has been reported in Inula anatolica, Rhinacanthus nasutus, and other organisms with relevant data. See also: Spongosterol monoglucoside (note moved to).
1. Stigmasterol glucoside is a steroidal glycoside compound that can be extracted from plant biomass (including Asili tea), and has dual functions of reduction and end-capping in the green synthesis of silver nanoparticles[1]. 2. The stigmasterol glucoside sequential injection-chemiluminescence method established in the literature [1] has the advantages of high sensitivity (detection limit 0.02 μM), good linear range (0.05-10 μM) and strong selectivity, providing a rapid and green detection method for this compound[1]. |
| Molecular Formula |
C35H58O6
|
|---|---|
| Molecular Weight |
574.8314
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| Exact Mass |
574.423
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| CAS # |
19716-26-8
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| PubChem CID |
6602508
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| Appearance |
White to off-white solid powder
|
| Density |
1.1±0.1 g/cm3
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| Boiling Point |
673.6±55.0 °C at 760 mmHg
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| Melting Point |
299 °C
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| Flash Point |
361.2±31.5 °C
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| Vapour Pressure |
0.0±4.7 mmHg at 25°C
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| Index of Refraction |
1.562
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| LogP |
8.26
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
41
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| Complexity |
962
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| Defined Atom Stereocenter Count |
14
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| SMILES |
CC[C@H](/C=C/[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC[C@@H](C4)O[C@H]5[C@@H]([C@H]([C@@H]([C@H](O5)CO)O)O)O)C)C)C(C)C
|
| InChi Key |
VWDLOXMZIGUBKM-AUGXRQBFSA-N
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| InChi Code |
InChI=1S/C35H58O6/c1-7-22(20(2)3)9-8-21(4)26-12-13-27-25-11-10-23-18-24(14-16-34(23,5)28(25)15-17-35(26,27)6)40-33-32(39)31(38)30(37)29(19-36)41-33/h8-10,20-22,24-33,36-39H,7,11-19H2,1-6H3/b9-8+/t21-,22-,24+,25+,26-,27+,28+,29-,30-,31+,32-,33-,34+,35-/m1/s1
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| Chemical Name |
(2R,3R,4S,5S,6R)-2-[[(3S,8S,9S,10R,13R,14S,17R)-17-[(E,2R,5S)-5-ethyl-6-methylhept-3-en-2-yl]-10,13-dimethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-yl]oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7396 mL | 8.6982 mL | 17.3964 mL | |
| 5 mM | 0.3479 mL | 1.7396 mL | 3.4793 mL | |
| 10 mM | 0.1740 mL | 0.8698 mL | 1.7396 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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