| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
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| ln Vitro |
In canine tracheal epithelium, stepronin (0.1-100 µM; 20 minutes) dramatically lowers ISOP (isoproterenol)-induced SCC (short circuit current) values in a dose-dependent manner [1]. In canine tracheal epithelium, stepronin (10-100 µM; 20 minutes) substantially and dose-dependently suppresses basal secretion and ISOP-induced PD (potential difference) values [1]. Stepronin (100 µM; 20 min) dramatically reduces the amount of [3H]-glycoconjugates that submucosal glands secrete in response to ISOP [1].
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| Cell Assay |
Cell Viability Assay[1]
Cell Types: Canine tracheal epithelium (from adult dogs and male-female mongrel dogs weighing 10 to 25 kg) Tested Concentrations: 0.1-100 µM; 10-100 µM Incubation Duration: 20 minutes (pretreatment) Experimental Results: Significant Dramatically inhibited ISOP-induced SCC and PD values as well as basal secretion. Cell viability assay[1] Cell Types: Submucosal gland (from adult and male-female mongrel dogs weighing 10 to 25 kg) Tested Concentrations: 100 µM Incubation Duration: 20 minutes (pre-treatment) Experimental Results: Significant inhibition of ISOP-induced [3H ]-glycoconjugate secretion. |
| References | |
| Additional Infomation |
Stepronin is a N-acyl-amino acid.
Strepronin is a mucolytic drug. A mucolytic agent is any agent which dissolves thick mucus usually used to help relieve respiratory difficulties. The viscosity of mucous secretions in the lungs is dependent upon the concentrations of mucoprotein as well as the presence of disulfide bonds between these macromolecules and DNA. Drug Indication Strepronin is a mucolytic (expectorant) drug. Mechanism of Action An expectorant increases bronchial secretions and mucolytics help loosen thick bronchial secretions. Expectorants reduce the thickness or viscosity of bronchial secretions thus increasing mucus flow that can be removed more easily through coughing, Mucolytics break down the chemical structure of mucus molecules. The mucus becomes thinner and can be removed more easily through coughing. Pharmacodynamics The drug promotes drainage of mucus from the lungs by thinning the mucus and lubricating the irritated respiratory tract |
| Molecular Formula |
C10H11NO4S2
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|---|---|
| Molecular Weight |
273.32864
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| Exact Mass |
273.013
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| CAS # |
72324-18-6
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| Related CAS # |
78126-10-0 (sodium);72324-18-6 (free acid);113790-28-6 (lysine salt);
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| PubChem CID |
54120
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| Appearance |
White to off-white solid powder
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| Density |
1.428 g/cm3
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| Boiling Point |
521.2ºC at 760 mmHg
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| Melting Point |
168-170ºC
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| Flash Point |
269ºC
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| Index of Refraction |
1.609
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| LogP |
1.601
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
17
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| Complexity |
321
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| Defined Atom Stereocenter Count |
0
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| SMILES |
OC(CNC(C(SC(C1=CC=CS1)=O)C)=O)=O
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| InChi Key |
JNYSEDHQJCOWQU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H11NO4S2/c1-6(9(14)11-5-8(12)13)17-10(15)7-3-2-4-16-7/h2-4,6H,5H2,1H3,(H,11,14)(H,12,13)
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| Chemical Name |
2-[2-(thiophene-2-carbonylsulfanyl)propanoylamino]acetic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 250 mg/mL (~914.65 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6586 mL | 18.2929 mL | 36.5858 mL | |
| 5 mM | 0.7317 mL | 3.6586 mL | 7.3172 mL | |
| 10 mM | 0.3659 mL | 1.8293 mL | 3.6586 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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