| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
In vitro, sphingosine is involved in regulating apoptosis, cell growth, and signal transduction. It can be phosphorylated by sphingosine kinases to produce sphingosine-1-phosphate (S1P), a potent signaling molecule. Sphingosine and S1P modulate various cellular processes including cell survival, proliferation, migration, and inflammation. However, specific in vitro activity data for the d17:1 form are limited.
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| ln Vitro |
In vivo, sphingosine is a naturally occurring lipid involved in sphingolipid signaling pathways. It is found in human skin and other tissues. Sphingosine and its phosphorylated metabolite S1P play important roles in skin barrier function, immune regulation, and metabolic homeostasis. However, specific in vivo data for the d17:1 form are limited.
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| Enzyme Assay |
There are no specific in vitro enzyme or receptor binding assays for sphingosine as a drug target. As a lipid, it is typically studied in sphingolipid metabolism assays. Sphingosine kinase activity can be measured by incubating sphingosine with ATP and sphingosine kinase, and quantifying S1P production by HPLC or mass spectrometry.
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| Cell Assay |
There are no specific in vitro cellular assays for sphingosine as a pharmacologically active compound. It is commonly used as an internal standard for sphingolipid analysis by mass spectrometry. In cell culture, sphingosine can be added to cells to study sphingolipid signaling pathways, apoptosis, and cell growth.
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| Animal Protocol |
There are no specific in vivo animal studies for sphingosine as a pharmacologically active compound. It is a naturally occurring lipid and is not administered as a drug.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of sphingosine are not typically studied as it is an endogenous lipid. As a lipophilic molecule, it is incorporated into cell membranes and metabolized by sphingosine kinases and lyases.
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| Toxicity/Toxicokinetics |
The toxicity profile of sphingosine is not extensively reported as it is an endogenous lipid. At high concentrations, sphingosine can induce apoptosis. The compound is for research use only and not for human therapeutic use.
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| References |
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| Additional Infomation |
C17 sphingosine is a C17 analog of sphingosine. It is an aminodiol and a sphingosine. It is the conjugate base of C17 sphingosine (1+).
Sphingosine (CAS 6918-48-5, d17:1) is a 17-carbon sphingolipid found in human skin that serves as a precursor for sphingosine-1-phosphate (S1P). It is involved in regulating apoptosis, cell growth, and signal transduction. The compound is commonly used as an internal standard for sphingolipid analysis by mass spectrometry. It is available for research purposes only. |
| Molecular Formula |
C17H35NO2
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|---|---|
| Molecular Weight |
285.4653
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| Exact Mass |
285.266
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| CAS # |
6918-48-5
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| PubChem CID |
5283557
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| Appearance |
White to off-white solid powder
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| Density |
0.9±0.1 g/cm3
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| Boiling Point |
432.9±45.0 °C at 760 mmHg
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| Flash Point |
215.6±28.7 °C
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| Vapour Pressure |
0.0±2.3 mmHg at 25°C
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| Index of Refraction |
1.490
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| LogP |
5.87
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
14
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| Heavy Atom Count |
20
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| Complexity |
219
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| Defined Atom Stereocenter Count |
2
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| SMILES |
CCCCCCCCCCCC/C=C/[C@H]([C@H](CO)N)O
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| InChi Key |
RBEJCQPPFCKTRZ-LHMZYYNSSA-N
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| InChi Code |
InChI=1S/C17H35NO2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-17(20)16(18)15-19/h13-14,16-17,19-20H,2-12,15,18H2,1H3/b14-13+/t16-,17+/m0/s1
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| Chemical Name |
(E,2S,3R)-2-aminoheptadec-4-ene-1,3-diol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5030 mL | 17.5150 mL | 35.0300 mL | |
| 5 mM | 0.7006 mL | 3.5030 mL | 7.0060 mL | |
| 10 mM | 0.3503 mL | 1.7515 mL | 3.5030 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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