| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Sortin1 targets the vacuolar protein sorting pathway, specifically affecting the trafficking of carboxypeptidase Y (CPY) and other vacuolar proteins. It acts by redirecting the vacuolar destination of plant CPY and other proteins in Arabidopsis suspension cells, causing these proteins to be secreted.
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| ln Vitro |
Sortin1 is a vacuolar protein sorting inhibitor that targets carboxypeptidase Y (CPY). It causes specific mislocalization of plant and yeast soluble and membrane vacuolar markers. The compound is a soluble and membrane vacuolar marker molecule in plants and yeast.
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| ln Vivo |
Specific in vivo efficacy data for Sortin1 are not applicable as it is a chemical genetic tool used primarily for studying protein trafficking pathways in plants and yeast. Its activity is assessed by monitoring the mislocalization of vacuolar proteins in Arabidopsis suspension cells and yeast models.
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| Enzyme Assay |
Sortin1's activity can be assessed using in vitro protein trafficking assays in plant or yeast systems. Arabidopsis suspension cells or yeast cells are treated with Sortin1, and the localization of vacuolar marker proteins (e.g., carboxypeptidase Y) is monitored using fluorescent protein fusions or immunofluorescence microscopy. Secretion of vacuolar proteins into the culture medium is measured by Western blot or enzyme activity assays.
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| Cell Assay |
The cellular activity of Sortin1 is evaluated in plant (Arabidopsis) or yeast cell cultures. Cells are treated with increasing concentrations of Sortin1, and the localization of vacuolar markers is assessed by confocal microscopy. The amount of secreted CPY or other vacuolar proteins in the culture medium is measured by enzymatic activity or Western blot. Cell viability is monitored to ensure that effects are not due to cytotoxicity.
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| Animal Protocol |
Sortin1 is typically used in plant or yeast cell culture models for studying vacuolar protein sorting. In Arabidopsis suspension cells or yeast cultures, Sortin1 is added to the culture medium at defined concentrations. Cells are incubated for a specified period, and protein trafficking is assessed by microscopy or biochemical analysis of secreted proteins.
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| ADME/Pharmacokinetics |
Sortin1 has a molecular formula of C26H19NO6 and a molecular weight of 441.43 g/mol. Its chemical name is 4-{5-[3-(methoxycarbonyl)-2-methyl-5-oxo-1H,4H,5H-indeno[1,2-b]pyridin-4-yl]furan-2-yl}benzoic acid. Specific pharmacokinetic parameters are not applicable.
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| Toxicity/Toxicokinetics |
The toxicity profile of Sortin1 is not extensively documented. As a chemical genetic tool used in plant and yeast models, its toxicity is assessed by monitoring cell viability in treated cultures. Specific LD50 values and mammalian toxicity data are not applicable.
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| Additional Infomation |
Sortin1 (CAS# 503837-98-7) is a vacuolar protein sorting inhibitor that targets carboxypeptidase Y (CPY). It redirects the vacuolar destination of plant CPY and other proteins, causing them to be secreted. Sortin1 is a chemical genetic tool for studying protein trafficking and vacuolar sorting pathways in plants and yeast.
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| Molecular Formula |
C26H19NO6
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|---|---|
| Molecular Weight |
441.43
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| Exact Mass |
441.121
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| CAS # |
503837-98-7
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| PubChem CID |
2888976
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| Appearance |
Orange to red solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
675.4±55.0 °C at 760 mmHg
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| Flash Point |
362.3±31.5 °C
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| Vapour Pressure |
0.0±2.2 mmHg at 25°C
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| Index of Refraction |
1.696
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| LogP |
4.07
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
33
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| Complexity |
912
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O1C(C2C([H])=C([H])C(C(=O)O[H])=C([H])C=2[H])=C([H])C([H])=C1C1([H])C(C(=O)OC([H])([H])[H])=C(C([H])([H])[H])N([H])C2C3=C([H])C([H])=C([H])C([H])=C3C(C1=2)=O
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| InChi Key |
QKEXVHYWWUXESV-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C26H19NO6/c1-13-20(26(31)32-2)21(22-23(27-13)16-5-3-4-6-17(16)24(22)28)19-12-11-18(33-19)14-7-9-15(10-8-14)25(29)30/h3-12,21,27H,1-2H3,(H,29,30)
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| Chemical Name |
4-[5-(3-methoxycarbonyl-2-methyl-5-oxo-1,4-dihydroindeno[1,2-b]pyridin-4-yl)furan-2-yl]benzoic acid
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| Synonyms |
Sortin 1; Sortin-1; Sortin1
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~226.54 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2654 mL | 11.3268 mL | 22.6536 mL | |
| 5 mM | 0.4531 mL | 2.2654 mL | 4.5307 mL | |
| 10 mM | 0.2265 mL | 1.1327 mL | 2.2654 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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