| Size | Price | Stock | Qty |
|---|---|---|---|
| 10g |
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| 25g |
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| Other Sizes |
| Targets |
Solvent Blue 35 does not have a specific pharmacological target. It is not a drug and is not intended for therapeutic use. It may be used as a staining agent in histological and biological research for the visualization of lipid-containing structures.
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|---|---|
| ln Vitro |
In vitro, Solvent Blue 35 is used as a lipid stain to detect neutral lipids in cells and tissues. It stains lipid droplets in adipocytes and other lipid-rich cells. It does not have intrinsic biological activity as a therapeutic agent.
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| ln Vivo |
In vivo, Solvent Blue 35 is used in animal studies as a tracer or marker for lipid absorption and transport. It can be administered orally with a lipid meal to track the distribution and metabolism of dietary fats.
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| Enzyme Assay |
For staining, tissue sections or cell cultures are fixed and incubated with a solution of Solvent Blue 35 in a suitable organic solvent (e.g., isopropanol or ethanol). The stain binds to neutral lipids, and excess stain is washed away. Stained lipids appear blue under light microscopy.
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| Cell Assay |
Adipocytes or other lipid-containing cells are cultured on coverslips. Cells are fixed with paraformaldehyde and stained with Solvent Blue 35 solution. After washing, coverslips are mounted and examined under a light microscope. Lipid droplets are visualized as blue-stained structures.
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| Animal Protocol |
Animal studies involve oral administration of Solvent Blue 35 mixed with a lipid meal. After a defined time, animals are sacrificed, and tissues (e.g., intestine, liver, adipose tissue) are harvested. Tissue sections are prepared and examined for the presence of the blue dye to track lipid absorption and distribution.
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| ADME/Pharmacokinetics |
Solvent Blue 35 (MW 350.45) is a lipophilic dye. It is absorbed with dietary lipids and transported in lipoproteins. It is deposited in adipose tissue and other lipid-rich organs. It is not metabolized to a significant extent and is excreted slowly. It is not used as a therapeutic agent.
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| Toxicity/Toxicokinetics |
Solvent Blue 35 has low acute toxicity. It is not classified as a hazardous substance for normal handling. However, as a synthetic dye, it may have potential long-term effects, and proper safety precautions should be followed. It is not intended for human consumption.
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| Additional Infomation |
Solvent Blue 35 is an industrial dye and not a pharmaceutical compound. It is used in research as a lipid stain and tracer. It is also known as Sudan Blue 2. It is not approved for therapeutic use and is not in clinical trials.
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| Molecular Formula |
C22H26N2O2
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|---|---|
| Molecular Weight |
350.45404
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| Exact Mass |
350.199
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| CAS # |
17354-14-2
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| PubChem CID |
3766139
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| Appearance |
Light blue to blue solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
568.7±50.0 °C at 760 mmHg
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| Melting Point |
120-122ºC(lit.)
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| Flash Point |
187.2±30.3 °C
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| Vapour Pressure |
0.0±1.6 mmHg at 25°C
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| Index of Refraction |
1.630
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| LogP |
4.93
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
26
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| Complexity |
449
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
OCQDPIXQTSYZJL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C22H26N2O2/c1-3-5-13-23-17-11-12-18(24-14-6-4-2)20-19(17)21(25)15-9-7-8-10-16(15)22(20)26/h7-12,23-24H,3-6,13-14H2,1-2H3
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| Chemical Name |
1,4-bis(butylamino)anthracene-9,10-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~41.67 mg/mL (~118.90 mM)
H2O : ~0.1 mg/mL (~0.29 mM) |
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8535 mL | 14.2674 mL | 28.5347 mL | |
| 5 mM | 0.5707 mL | 2.8535 mL | 5.7069 mL | |
| 10 mM | 0.2853 mL | 1.4267 mL | 2.8535 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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