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| 1mg |
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| 5mg |
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| Targets |
The primary target of Smcy HY Peptide (738-746) is the H2-Dᵇ major histocompatibility complex (MHC) class I molecule. The peptide binds to H2-Dᵇ and is presented on the cell surface, where it can be recognized by CD8+ T cells. This peptide is derived from the Smcy protein, which is encoded on the Y chromosome and is therefore a male-specific antigen. The Smcy HY peptide is a well-known model antigen for studying T cell responses, immune tolerance, and autoimmunity. The H2-Dᵇ-restricted nature of this peptide makes it a valuable tool for studying MHC class I antigen presentation in mice.
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| ln Vitro |
In vitro studies of Smcy HY Peptide (738-746) focus on its ability to bind to H2-Dᵇ MHC class I molecules and activate CD8+ T cells. The peptide is used in T cell activation assays to measure the proliferation, cytokine production, and cytotoxicity of antigen-specific T cells. It can be loaded onto antigen-presenting cells (APCs) expressing H2-Dᵇ and used to stimulate T cells from immunized or transgenic mice. The peptide's binding affinity for H2-Dᵇ can be measured using MHC stabilization assays or peptide-MHC binding assays. Smcy HY Peptide is a well-characterized model antigen that is widely used in immunology research to study T cell responses and immune regulation.
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| ln Vivo |
In vivo studies of Smcy HY Peptide (738-746) are typically conducted in mouse models to study T cell responses, immune tolerance, and transplantation immunity. The peptide can be administered to mice to induce T cell responses, and the resulting immune responses can be characterized by measuring T cell proliferation, cytokine production, and cytotoxicity. The peptide is used in models of male-specific immune responses, as Smcy is a Y chromosome-encoded antigen. It is also used in studies of immune tolerance and autoimmunity, as the HY antigen is a model for self/non-self discrimination. Smcy HY Peptide is valuable for studying CD8+ T cell biology in vivo.
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| Enzyme Assay |
For in vitro enzyme/receptor binding assays, Smcy HY Peptide (738-746) can be evaluated using MHC class I binding assays to measure its affinity for H2-Dᵇ. These assays typically involve incubating the peptide with H2-Dᵇ molecules (either purified or expressed on the surface of cells) and measuring peptide-MHC complex formation using conformation-specific antibodies or by measuring MHC stabilization on the cell surface. Surface plasmon resonance (SPR) can be used to measure the binding affinity and kinetics of the peptide-MHC interaction. Peptide-MHC tetramers can be generated using this peptide for staining antigen-specific T cells. Standard assay conditions include physiological buffer systems with appropriate pH and ionic strength.
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| Cell Assay |
For in vitro cellular experiments, Smcy HY Peptide (738-746) is typically used in T cell activation assays. Antigen-presenting cells (such as dendritic cells, macrophages, or B cells) expressing H2-Dᵇ are loaded with the peptide and co-cultured with CD8+ T cells from mice that recognize the Smcy HY antigen. T cell activation is measured by assessing proliferation (using [3H]-thymidine incorporation or CFSE dilution), cytokine production (by ELISA or intracellular cytokine staining), and cytotoxicity (using ⁵¹Cr release assays or other cytotoxicity assays). The peptide can also be used to generate peptide-MHC tetramers for flow cytometric detection of antigen-specific T cells.
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| Animal Protocol |
For in vivo animal experiments, Smcy HY Peptide (738-746) is administered to mice to induce or modulate T cell responses. The peptide can be injected subcutaneously, intraperitoneally, or intravenously, often with adjuvant (such as complete Freund's adjuvant) to enhance immunogenicity. Alternatively, the peptide can be loaded onto dendritic cells ex vivo and then injected into mice for immunization. Immune responses are evaluated by measuring T cell proliferation, cytokine production, and cytotoxicity. The peptide is also used in models of transplantation and autoimmunity to study T cell-mediated rejection or tolerance. Animal studies should follow appropriate ethical guidelines.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Smcy HY Peptide (738-746) have not been extensively characterized, as it is primarily used as an immunological research tool rather than a drug candidate. As a peptide with a molecular weight of 1167.34 g/mol, it would be expected to have low oral bioavailability and rapid clearance by proteases. When administered systemically, the peptide would likely be rapidly degraded by peptidases in the blood and tissues. Its half-life in circulation would be short. For in vivo immunization studies, the peptide is typically administered with adjuvants to enhance its immunogenicity and prolong its presentation. The peptide's stability in solution may be limited, and storage at -20degC is recommended.
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| Toxicity/Toxicokinetics |
Toxicological data for Smcy HY Peptide (738-746) are limited, as it is a research peptide used in immunological studies. As a peptide derived from a self-protein, it is generally considered to have low toxicity at the concentrations used for research. However, when administered with strong adjuvants, it can induce robust immune responses that may lead to inflammation or tissue damage. Standard toxicological assessments would include evaluation of immune-mediated adverse effects. As with all research peptides, appropriate safety precautions should be taken when handling Smcy HY Peptide, including the use of personal protective equipment and proper laboratory practices. The peptide should be stored appropriately to maintain stability.
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| References | |
| Additional Infomation |
Smcy HY Peptide (738-746) is a research peptide used in immunology to study MHC class I antigen presentation and CD8+ T cell responses. No clinical trials or regulatory approvals have been reported for this peptide as a therapeutic agent. It is available from various peptide suppliers for research purposes only. The peptide is a well-characterized model antigen for studying T cell responses, immune tolerance, and transplantation immunity. Its H2-Dᵇ restriction and Y chromosome origin make it a valuable tool for studying male-specific immune responses and self/non-self discrimination. The peptide is typically stored at -20degC, protected from light, and kept dry and sealed.
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| Exact Mass |
1166.597
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| CAS # |
207113-06-2
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| PubChem CID |
162678805
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| Appearance |
White to off-white solid powder
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| LogP |
-8.9
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| Hydrogen Bond Donor Count |
20
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| Hydrogen Bond Acceptor Count |
19
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| Rotatable Bond Count |
40
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| Heavy Atom Count |
81
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| Complexity |
2160
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| Defined Atom Stereocenter Count |
9
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| SMILES |
CC(C)C[C@@H](C(=O)O)NC(=O)[C@H](CC1=CC=C(C=C1)O)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(=O)N)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CO)NC(=O)[C@H](CS)NC(=O)[C@H](CCCCN)N
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| InChi Key |
AGWYNBDLJBJVJU-AGQURRGHSA-N
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| InChi Code |
InChI=1S/C48H82N18O14S/c1-24(2)19-33(46(79)80)64-42(75)31(20-25-10-12-26(68)13-11-25)62-41(74)30(14-15-36(51)69)61-39(72)28(8-5-17-57-47(53)54)59-43(76)32(21-37(52)70)63-40(73)29(9-6-18-58-48(55)56)60-44(77)34(22-67)65-45(78)35(23-81)66-38(71)27(50)7-3-4-16-49/h10-13,24,27-35,67-68,81H,3-9,14-23,49-50H2,1-2H3,(H2,51,69)(H2,52,70)(H,59,76)(H,60,77)(H,61,72)(H,62,74)(H,63,73)(H,64,75)(H,65,78)(H,66,71)(H,79,80)(H4,53,54,57)(H4,55,56,58)/t27-,28-,29-,30-,31-,32-,33-,34-,35-/m0/s1
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| Chemical Name |
(2S)-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-4-amino-2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S)-2,6-diaminohexanoyl]amino]-3-sulfanylpropanoyl]amino]-3-hydroxypropanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]amino]-4-oxobutanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]amino]-5-oxopentanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-4-methylpentanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~85.66 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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