| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
Based on a considerable rise in the RFP/BFP ratio, SID 3712249 (compound 1, 20 nM, 48 hours) disturbs the regulation of BMI1 mediated by miR-544 [1]. Precursor miR-544 binding to BMI1 or mTOR3'-UTR is inhibited by SID 3712249 (20 nM, 48 hours), but not mature miR-544 binding [1]. In MCF10A cells, SID 3712249 (20 nM, 48 hours) causes pre-miR-544 to accumulate and miR-544 levels to decrease [1]. Under normoxic conditions, SID 3712249 (20 nM, 5 days) causes apoptosis in MDA-MB-231 and MCF-7 cells but has little effect on cell survival [1].
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| ln Vivo |
In the MDA-MB-231-GFP-luc tumor model, SID 3712249 (Compound 1, 100 μL, 40 μM, intraperitoneal injection) suppresses tumor development [1]. In the MDA-MB-231-GFP-luc tumor model, SID 3712249 (20 nM, intraperitoneally injected, pretreatment GFP-labeled MDA-MB-231 cells) reduces tumor growth [1].
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| Cell Assay |
Apoptosis analysis [1]
Cell Types: MDA-MB-231, MCF-7 Cell Tested Concentrations: 20 nM Incubation Duration: 5 days Experimental Results: Induced apoptosis under normoxic conditions with no effect on cell survival (Annexin V and Flow cytometry analysis of PI) staining). |
| Animal Protocol |
Animal/Disease Models: MDA-MB-231-GFP-luc tumor model [1]
Doses: 100 μL 40 μM Route of Administration: intraperitoneal (ip) injection Experimental Results: Inhibition of tumor growth (proven by bioluminescence imaging in living animals), no obvious side effects. miR-544, ATM, and HIF-1α levels were diminished, and mTOR levels were increased (resected tumor samples). |
| References |
[1]. Christopher L Haga, et al. Small Molecule Inhibition of miR-544 Biogenesis Disrupts Adaptive Responses to Hypoxia by Modulating ATM-mTOR Signaling. ACS Chem Biol. 2015 Oct 16;10(10):2267-76.
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| Molecular Formula |
C17H21N7
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|---|---|
| Molecular Weight |
323.396
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| Exact Mass |
323.185
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| CAS # |
522606-67-3
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| PubChem CID |
2997948
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
669.0±55.0 °C at 760 mmHg
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| Flash Point |
358.4±31.5 °C
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| Vapour Pressure |
0.0±2.0 mmHg at 25°C
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| Index of Refraction |
1.731
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| LogP |
2.27
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
24
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| Complexity |
490
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| Defined Atom Stereocenter Count |
0
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| SMILES |
NC1=C2C(C=C(N3CCCC3)N=C2N)=C(C#N)C(N4CCCC4)=N1
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| InChi Key |
SSWVPYMSHHEYAB-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H21N7/c18-10-12-11-9-13(23-5-1-2-6-23)21-15(19)14(11)16(20)22-17(12)24-7-3-4-8-24/h9H,1-8H2,(H2,19,21)(H2,20,22)
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| Chemical Name |
1,8-diamino-3,6-dipyrrolidin-1-yl-2,7-naphthyridine-4-carbonitrile
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~16.67 mg/mL (~51.55 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0921 mL | 15.4607 mL | 30.9215 mL | |
| 5 mM | 0.6184 mL | 3.0921 mL | 6.1843 mL | |
| 10 mM | 0.3092 mL | 1.5461 mL | 3.0921 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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