| Size | Price | Stock | Qty |
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| 25g |
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| 50g |
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| 100g |
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| 200g |
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| Other Sizes |
Purity: ≥98%
| Targets |
Sericin targets various biological processes rather than specific molecular receptors. It binds tightly to keratin, imparting immediate and enduring smoothness. Sericin inhibits tyrosinase activity, preventing skin darkening through melanin production inhibition. Tryptophan and phenylalanine in sericin absorb ultraviolet light, protecting skin from sun damage and tanning. The protein reduces transepidermal water loss (TEWL) and provides long-lasting moisturizing benefits. Sericin's biological effects are primarily mediated through its interactions with cell surfaces and extracellular matrix components rather than through specific receptor binding, contributing to its diverse biological activities including cell growth promotion and cell death inhibition.
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| ln Vitro |
In vitro, sericin has demonstrated multiple beneficial activities for mammalian cell culture. It can act as a FBS replacement in cell culture and stimulate cell growth while inhibiting cell death in certain situations. Sericin exhibits cryoprotective properties that allow for the replacement of FBS in cryopreservation media. The protein has been shown to prevent cell adhesion, growth, and spread of cell colonies. Sericin also has antiviral activity, inhibiting microbial and viral adhesion and entry into host cells. In cosmetic applications, sericin enhances skin smoothness, reduces TEWL, and provides moisturizing benefits. Studies have shown that sericin inhibits tyrosinase activity even after boiling treatment.
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| ln Vivo |
In vivo, sericin has been studied for its dermatological and biomedical applications. It forms a semi-occlusive protective film on the skin, enhancing smoothness and reducing transepidermal water loss. Sericin provides long-lasting moisturizing and anti-wrinkle benefits. Its ability to absorb UV radiation through tryptophan and phenylalanine suggests photoprotective effects. The protein's inhibition of tyrosinase activity indicates potential skin-lightening effects. Sericin has been shown to enhance skin elasticity and reduce the appearance of fine lines. Its gentle, non-irritating nature makes it suitable for sensitive skin products. In biomedical applications, sericin is used in wound healing, tissue engineering, and drug delivery systems due to its biocompatibility and bioactivity.
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| Enzyme Assay |
For in vitro biochemical assays, sericin's biological activities can be assessed using various cell-free and cell-based methods. Tyrosinase inhibition assays are performed using mushroom tyrosinase and L-DOPA as substrate, measuring melanin formation spectrophotometrically. Antioxidant activity can be assessed using DPPH, ABTS, or FRAP assays. Protein concentration is measured using Bradford or BCA assays. UV absorption spectroscopy can be used to assess the compound's photoprotective properties. Cell adhesion assays can be performed using purified sericin-coated surfaces to study cell-substrate interactions. These cell-free assays help characterize sericin's biochemical properties and identify the molecular basis of its biological activities.
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| Cell Assay |
In vitro cellular assays for sericin are performed using various mammalian cell lines. Fibroblasts, keratinocytes, and other skin-related cells are commonly used for dermatological studies. Cells are cultured in media supplemented with sericin at various concentrations. Cell proliferation is assessed using MTT, CCK-8, or BrdU incorporation assays. Cell viability and cytotoxicity are evaluated using LDH release or trypan blue exclusion. Cell adhesion and spreading are assessed using microscopy and image analysis. Cryopreservation studies evaluate the protective effects of sericin on cell viability after freeze-thaw cycles. Anti-inflammatory activity can be assessed by measuring cytokine production in response to inflammatory stimuli. Wound healing assays using scratch or transwell migration models are also performed.
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| Animal Protocol |
In vivo animal experiments with sericin are conducted to evaluate its dermatological and biomedical applications. Rodent models are commonly used for skin studies. Sericin is applied topically as creams, gels, or solutions to evaluate its effects on skin hydration, transepidermal water loss (TEWL), elasticity, and wrinkle formation. Wound healing models are used to assess sericin's effects on tissue regeneration and repair. For photoprotection studies, animals are exposed to UV radiation with or without sericin application, and skin damage is assessed histologically. Biocompatibility studies involve subcutaneous implantation of sericin-based materials to evaluate tissue response. For anti-inflammatory studies, animal models of dermatitis or other inflammatory skin conditions are used.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of sericin are characteristic of large proteins. As a glycoprotein derived from Bombyx mori, sericin has a high molecular weight and is not significantly absorbed through intact skin. When applied topically, it forms a film on the skin surface and exerts its effects locally. For systemic administration, sericin would be subject to proteolytic degradation and rapid clearance. The protein's stability in solution and powder form has been characterized for formulation purposes. Detailed PK parameters such as bioavailability, half-life, and distribution are not extensively documented as sericin is primarily used in topical and cell culture applications rather than as a systemically administered therapeutic.
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| Toxicity/Toxicokinetics |
The toxicological profile of sericin is generally favorable, as it is a natural protein derived from silkworm cocoons. It is described as gentle and non-irritating, making it suitable for sensitive skin products. However, individual hypersensitivity or allergic reactions to silk proteins may occur. Comprehensive toxicity studies including acute, subchronic, and chronic toxicity assessments are limited. Sericin is intended for research and cosmetic applications rather than systemic therapeutic use. As with any protein, appropriate safety assessments should be conducted for specific applications. The compound should be handled using standard laboratory safety practices.
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| Additional Infomation |
Sericin is widely used in cell culture applications as a FBS replacement and cryoprotective agent. Its ability to stimulate cell growth and inhibit cell death makes it valuable for biomanufacturing and regenerative medicine research. In cosmetic science, sericin is used for its moisturizing, anti-wrinkle, and skin-smoothing properties. Its UV-absorbing and tyrosinase-inhibiting activities make it relevant for photoprotection and skin-lightening research. Sericin is also used in wound healing, tissue engineering scaffolds, and drug delivery systems due to its biocompatibility and bioactivity. The protein's natural origin and gentle nature make it attractive for developing biocompatible and sustainable biomaterials.
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| Molecular Weight |
0
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| CAS # |
60650-88-6
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| Appearance |
Typically exists as solid at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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