Size | Price | Stock | Qty |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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500mg |
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Other Sizes |
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Purity: ≥98%
Rutaecarpine (also known as rutecarpine; Rutacarpine; Rutaecarpin; Rhetine) is a naturally occuring indolopyridoquinazolinone alkaloid isolated from isolated from the chinese herb Evodia rutaecarpa which has vasorelaxing effect and inhibits platelet aggregation and Cox-2. It is alos a selective inhibitor of CYP1A1(cytochrome P450 1A).
ln Vitro |
Numerous intriguing biological characteristics of rupine have been demonstrated, including impacts on the endocrine and cardiovascular systems, antithrombotic, anticancer, anti-inflammatory and analgesic, anti-obesity and thermoregulatory, and vasodilatory activities [2]. With an IC50 of 0.28 μM and 8.7 μM, respectively, rutaecarpine suppresses the COX-2 and COX-1-dependent phases of PGD2 synthesis in BMMC in a concentration-dependent manner. It inhibits in a dose-dependent manner the COX-2-dependent conversion of exogenous arachidonic acid to PGE2 by COX-2-transfected HEK293 cells [1].
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ln Vivo |
Rats' paw edema caused by L-carrageenan was treated with an intraperitoneal injection of evodiamine, which demonstrated in vivo anti-inflammatory action [1]. Rupine induced a dose-dependent decrease in the number of antibody-forming cells as well as a drop in spleen weight. Furthermore, animals with evodiamine displayed decreased spleen cellularity as well as decreased splenic total T cells, CD4+ cells, CD8+ cells, and B cells. IL-2, interferon, and IL-10 mRNA expression were all markedly suppressed by evodiamine therapy. Following rupine administration to mice, there was a considerable decrease in the quantity of CD4+IL-2+ cells [3].
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References |
[1]. Moon TC, et al. A new class of COX-2 inhibitor, rutaecarpine from Evodia rutaecarpa. Inflamm Res. 1999 Dec;48(12):621-5.
[2]. Lee SH, et al. Progress in the studies on rutaecarpine. Molecules. 2008 Feb 6;13(2):272-300. [3]. Jeon TW, et al. Immunosuppressive effects of rutaecarpine in female BALB/c mice. Toxicol Lett. 2006 Jul 1;164(2):155-66 |
Molecular Formula |
C18H13N3O
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Molecular Weight |
287.32
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CAS # |
84-26-4
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Related CAS # |
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SMILES |
O=C1C2=C([H])C([H])=C([H])C([H])=C2N=C2C3=C(C4=C([H])C([H])=C([H])C([H])=C4N3[H])C([H])([H])C([H])([H])N21
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InChi Key |
ACVGWSKVRYFWRP-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C18H13N3O/c22-18-13-6-2-4-8-15(13)20-17-16-12(9-10-21(17)18)11-5-1-3-7-14(11)19-16/h1-8,19H,9-10H2
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Chemical Name |
8,13-dihydro-indolo[2,3:3,4]pyrido[2,1-b]quinazolin-5(7H)-one
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Synonyms |
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.4804 mL | 17.4022 mL | 34.8044 mL | |
5 mM | 0.6961 mL | 3.4804 mL | 6.9609 mL | |
10 mM | 0.3480 mL | 1.7402 mL | 3.4804 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.