| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| Other Sizes |
| Targets |
radical scavenger
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|---|---|
| ln Vitro |
Rubrofusarin-6-β-D-gentiobioside exhibited radical scavenging activity on DPPH with an IC50 value of 18.04 μg/4ml (amount required for 50% reduction of DPPH after 30 minutes). The scavenging activity was measured spectrophotometrically at 517 nm. Among ten isolated compounds from Cassia tora, this compound showed higher scavenging activity, with an IC50 value of 18.04 μg/4ml, compared to L-ascorbic acid (IC50 8.1 μg) and BHT (IC50 9.5 μg). [1]
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| Enzyme Assay |
The DPPH radical scavenging activity assay was performed as follows: A 4 ml methanol solution of the test compound at various concentrations (2.5 - 120 μg/ml) was added to a 1 ml solution of DPPH (1.5 × 10⁻⁴ M) in methanol. The reaction mixture was shaken vigorously and then stored at room temperature for 30 minutes in air. After incubation, the remaining DPPH was determined by measuring absorbance at 517 nm using a spectrophotometer. The radical scavenging activity (%) of each sample was calculated as the ratio of the decrease in absorbance of DPPH relative to the absorbance of a control DPPH solution (without test sample). Mean values were obtained from duplicate experiments. The concentration required for 50% reduction of DPPH (IC50) was determined. [1]
|
| References | |
| Additional Infomation |
Reports indicate that Rubrofusarin gentiobioside has been found in Berchemia floribunda, Sennatora, and Berchemia racemosa, and relevant data are available for reference.
Rubrofusarin-6-β-D-gentiobioside (also referred to as Rubrofusarin-6-β-D-gentiobioside) was isolated from the n-butanol extract of Cassia tora seeds. The isolation procedure involved silica gel column chromatography of the butanol extract (90 g) with mixtures of ethyl acetate and methanol of increasing polarity, yielding 17 fractions. Fractions 6 and 7 were combined and rechromatographed with ethyl acetate-MeOH-H₂O (300:35:10) to afford the compound (50 mg). The compound was characterized as yellowish needles from methanol with a melting point of 186-188°C. Its structure was elucidated by ¹H NMR (300 MHz, DMSO-d₆) showing two anomeric proton signals at δ 5.05 (J=7.5 Hz) and δ 5.06 (J=7.5 Hz), a methyl signal at δ 2.38, a methoxyl signal at δ 3.88, and four aromatic protons at δ 6.18 (H-3), 6.81 (H-7), 6.93 (H-9) and 7.18 (H-10). Acid hydrolysis yielded an aglycone identified as rubrofusarin and D-glucose. ¹³C NMR data supported that the disaccharide moiety (gentiobiose, a β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside) was attached at the 6-position of rubrofusarin. The compound was previously isolated from the same plant by Wong et al. (1989) and Kaneda et al. (1969). [1] |
| Molecular Formula |
C27H32O15
|
|---|---|
| Molecular Weight |
596.5340
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| Exact Mass |
596.174
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| CAS # |
24577-90-0
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| PubChem CID |
503733
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| Appearance |
Light yellow to yellow solid
|
| Density |
1.7±0.1 g/cm3
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| Boiling Point |
914.7±65.0 °C at 760 mmHg
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| Flash Point |
302.9±27.8 °C
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| Vapour Pressure |
0.0±0.3 mmHg at 25°C
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| Index of Refraction |
1.719
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| Source |
Originated from plants: Leguminosae Seeds of Cassia tora Linn.
Originated from plants: other families |
| LogP |
-0.92
|
| Hydrogen Bond Donor Count |
8
|
| Hydrogen Bond Acceptor Count |
15
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
42
|
| Complexity |
979
|
| Defined Atom Stereocenter Count |
10
|
| SMILES |
O1C([H])(C([H])(C([H])(C([H])(C1([H])C([H])([H])OC1([H])C([H])(C([H])(C([H])(C([H])(C([H])([H])O[H])O1)O[H])O[H])O[H])O[H])O[H])O[H])OC1C([H])=C(C([H])=C2C([H])=C3C(C(C([H])=C(C([H])([H])[H])O3)=O)=C(C=12)O[H])OC([H])([H])[H]
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| InChi Key |
JIBJMBHKGBDCPN-IJTBWITGSA-N
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| InChi Code |
InChI=1S/C27H32O15/c1-9-3-12(29)18-13(39-9)5-10-4-11(37-2)6-14(17(10)21(18)32)40-27-25(36)23(34)20(31)16(42-27)8-38-26-24(35)22(33)19(30)15(7-28)41-26/h3-6,15-16,19-20,22-28,30-36H,7-8H2,1-2H3/t15-,16-,19-,20-,22+,23+,24-,25-,26-,27-/m1/s1
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| Chemical Name |
5-hydroxy-8-methoxy-2-methyl-6-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-[[(2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxymethyl]oxan-2-yl]oxybenzo[g]chromen-4-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~167.6 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (4.19 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.19 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6764 mL | 8.3818 mL | 16.7636 mL | |
| 5 mM | 0.3353 mL | 1.6764 mL | 3.3527 mL | |
| 10 mM | 0.1676 mL | 0.8382 mL | 1.6764 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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