Size | Price | Stock | Qty |
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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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500mg |
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Other Sizes |
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Purity: ≥98%
ln Vitro |
The rhodamine 123 working solution is prepared. 1.1 Making the stock solution Take 1 milligram of rhodamine 123 and dissolve it in 525 μL DMSO to make a 5 mM stock solution. 1.2 Making the working solution for rhodamine 123 To create a working solution ranging from 1 to 20 μM, utilize serum-free cell culture medium or PBS stock solution. Note: The concentration of the Rhodamine 123 working solution should be adjusted based on the specific circumstances. 2. The staining of cells In a 6-well plate, 2.1 suspended cells a. Discard the supernatant after centrifuging at 1000 g for three to five minutes at 4°C. c. After adding 1 mL of the working solution, observe for one to three hours. c. Centrifuge at 400 g for three to four minutes at 4 °C; remove supernatant. d. Wash twice, for five minutes each time. One x 10^6 cells per milliliter. Wash for five minutes each time, twice, using PBS. e. Re-suspend cells in PBS or serum-free culture medium. either flow cytometry monitoring or fluorescence microscopy. 2.2 Adherent cells Adherent cells should be cultured on sterile glass slides (a). a. Take off the coverslip from the culture medium and use an aspirator to remove any extra. c. Add 100 μL working solution, give the cells a gentle shake to cover them fully, and leave them in place for 30 to 60 minutes. d. Use medium and wash twice in about five minutes. either flow cytometry monitoring or fluorescence microscopy. Note: Prior to staining, the cells must be resuspended if flow cytometry is being employed for detection.
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References |
[1]. Emaus, R. K., Grunwald, R., & Lemasters, J. J. (1986). Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 850(3), 436–448.
[2]. M. Huang, et al. Mitochondrial Inner Membrane Electrophysiology Assessed by Rhodamine-123 Transport and Fluorescence. Ann Biomed Eng. 2007 Jul; 35(7): 1276–1285 |
Molecular Formula |
C21H17CLN2O3
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Molecular Weight |
344.3633
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CAS # |
62669-70-9
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SMILES |
O=C(C1=CC=CC=C1C2=C3C=CC(N)=CC3=[O+]C4=C2C=CC(N)=C4)OC.[Cl-]
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InChi Key |
TUFFYSFVSYUHPA-UHFFFAOYSA-M
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InChi Code |
InChI=1S/C21H17N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11H,22-23H2,1H31H/q+1/p-1
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Chemical Name |
Xanthylium, 3,6-diamino-9-(2-(methoxycarbonyl)phenyl)-, chloride
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Synonyms |
Rhodamine 123 R 22420 R 302 RH 123.
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~62.5 mg/mL (~164.12 mM)
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Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.9039 mL | 14.5197 mL | 29.0394 mL | |
5 mM | 0.5808 mL | 2.9039 mL | 5.8079 mL | |
10 mM | 0.2904 mL | 1.4520 mL | 2.9039 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.