| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
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| ln Vitro |
The pH of the mitochondria is altered as a result of rhodamine 110 building up there in the cationic form. Human lymphoblastoid and Friend leukemia cells accumulate rhodamine 110. At doses above 100 μM, rhodamine 110 triggered Friend leukemia cell death, while no cytotoxicity to human lymphoblastoid cells was seen below 10 μM [1].
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| ln Vivo |
Rhodamine 110 is less toxic than its parent molecule, according to the intravenous LD50 acute toxicity values of 89.5 mg/kg and 140.0 mg/kg, respectively, for both rhodamine B and rhodamine 110. When ingested, both molecules cause enlargement of the liver and kidney, with male rats exhibiting larger enlargements than female rats following exposure to rhodamine 110. Additionally, when male rats were given rhodamine 110, their testicular weight increased. Rhodamine 110 was administered orally at two doses (3 and 10 mg/kg) and intravenously at one dose (3 mg/kg) before its pharmacokinetics were assessed. WinNonlin Standard Edition was used for noncompartmental model analysis, and extravascular and intravenous bolus inputs were used to calculate pharmacokinetic parameters. The maximum plasma concentrations (Cmax) of the two oral doses of rhodamine 110 were found to be 283.4 and 657.0 ng/mL at 140 and 210 minutes, respectively, according to the pharmacokinetic parameters. This indicates that rhodamine 110 is not rapidly absorbed from the intestine into the bloodstream; rather, it takes over two hours. For both doses, the areas under the concentration-time curve (AUC) were, respectively, 138.1±20.3 and 444.0±170.8 h ng/mL. The AUC of rhodamine 110 (3 mg/kg and 10 mg/kg) is proportional to the oral dose, according to pharmacokinetic data. Furthermore, the two oral doses had respective clearance rates (CI) of 7.94 and 8.61 mL/min/kg [1].
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| References |
| Molecular Formula |
C20H15CLN2O3
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|---|---|
| Molecular Weight |
366.801
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| Exact Mass |
366.077
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| CAS # |
13558-31-1
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| Related CAS # |
82182-00-1 (cation);
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| PubChem CID |
11617572
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| Appearance |
Brown to red solid powder
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| Boiling Point |
629.3ºC at 760 mmHg
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| Melting Point |
>300 °C(lit.)
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| Flash Point |
334.4ºC
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| Vapour Pressure |
1.06E-16mmHg at 25°C
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| LogP |
5.447
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
26
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| Complexity |
691
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
JNGRENQDBKMCCR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C20H14N2O3.ClH/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24;/h1-10,21H,22H2,(H,23,24);1H
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| Chemical Name |
2-(3-amino-6-iminoxanthen-9-yl)benzoic acid;hydrochloride
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| Synonyms |
Rhodamine 110
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~30 mg/mL (~81.79 mM)
H2O : < 0.1 mg/mL |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7263 mL | 13.6314 mL | 27.2628 mL | |
| 5 mM | 0.5453 mL | 2.7263 mL | 5.4526 mL | |
| 10 mM | 0.2726 mL | 1.3631 mL | 2.7263 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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