| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Repinotan HCl primarily targets the 5-HT1A receptor, a subtype of serotonin receptor. It has a very high affinity for this receptor, with Ki values of 0.19 nM in calf hippocampus, 0.25 nM in rat and human cortex, and 0.59 nM in rat hippocampus. By acting as an agonist, it mimics the effects of serotonin at this receptor.
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|---|---|
| ln Vitro |
In vitro, Repinotan HCl's activity is characterized by its potent and selective binding to the 5-HT1A receptor. Its high affinity and selectivity make it a valuable tool for studying the role of this receptor in the central nervous system.
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| ln Vivo |
In vivo, Repinotan HCl has been investigated for its neuroprotective and neurorestorative properties. It has been primarily studied for the treatment of ischemic stroke and other central nervous system disorders. Its ability to penetrate the BBB is crucial for its central effects.
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| Enzyme Assay |
The binding affinity of Repinotan HCl to the 5-HT1A receptor is determined using radioligand binding assays. In these cell-free experiments, membrane preparations from tissues expressing the receptor are incubated with a radiolabeled ligand in the presence of varying concentrations of the compound. The displacement of the ligand is measured to calculate the Ki.
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| Cell Assay |
For cellular assays, cell lines expressing the human 5-HT1A receptor are used. The compound's ability to activate the receptor is measured by assessing its effect on downstream signaling pathways, such as the inhibition of adenylyl cyclase.
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| Animal Protocol |
In vivo, Repinotan HCl is typically administered orally or intravenously. Its efficacy is evaluated in animal models of stroke or other neurological conditions, where its neuroprotective effects are assessed by measuring infarct size or functional recovery.
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| ADME/Pharmacokinetics |
Repinotan HCl is an orally active compound. It is soluble in DMSO at ≥10 mg/mL. Its ability to penetrate the BBB is a key pharmacokinetic property.
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| Toxicity/Toxicokinetics |
Specific toxicity data for Repinotan HCl is not detailed in the search results. As a 5-HT1A agonist, its side effect profile would be related to serotonin receptor activation.
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| References | |
| Additional Infomation |
See also: Repinotan (Note moved to).
Repinotan HCl is a research compound that has been studied for its potential in treating neurological conditions such as ischemic stroke. Its development highlights the therapeutic potential of 5-HT1A receptor agonists in neuroprotection. It is not an approved drug. |
| Molecular Formula |
C21H24N2O4S.CLH
|
|---|---|
| Molecular Weight |
436.9522
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| Exact Mass |
436.122
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| CAS # |
144980-77-8
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| Related CAS # |
Repinotan;144980-29-0
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| PubChem CID |
198756
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| Appearance |
Light yellow to brown solid powder
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| Boiling Point |
599.5ºC at 760mmHg
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| Flash Point |
316.4ºC
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| Vapour Pressure |
2.49E-14mmHg at 25°C
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| LogP |
4.806
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
29
|
| Complexity |
647
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| Defined Atom Stereocenter Count |
1
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| SMILES |
C1=CC=C2C(=C1)CC[C@H](CNCCCCN3C(=O)C4=CC=CC=C4S3(=O)=O)O2.Cl
|
| InChi Key |
IGKYREHZJIHPML-UNTBIKODSA-N
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| InChi Code |
InChI=1S/C21H24N2O4S.ClH/c24-21-18-8-2-4-10-20(18)28(25,26)23(21)14-6-5-13-22-15-17-12-11-16-7-1-3-9-19(16)27-17;/h1-4,7-10,17,22H,5-6,11-15H2;1H/t17-;/m1./s1
|
| Chemical Name |
2-[4-[[(2R)-3,4-dihydro-2H-chromen-2-yl]methylamino]butyl]-1,1-dioxo-1,2-benzothiazol-3-one;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~228.9 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.72 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.72 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (5.72 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2886 mL | 11.4430 mL | 22.8859 mL | |
| 5 mM | 0.4577 mL | 2.2886 mL | 4.5772 mL | |
| 10 mM | 0.2289 mL | 1.1443 mL | 2.2886 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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