| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| Targets |
Rehmannioside D targets multiple pathways involved in oxidative stress and inflammation. It exhibits antioxidant properties, suggesting it can scavenge free radicals and reduce oxidative stress. It has anti-inflammatory properties, indicating it can reduce inflammation. It also has moisture-supporting properties. As an iridoid glycoside from Rehmannia glutinosa, it may modulate pathways related to these activities.
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| ln Vitro |
In vitro, Rehmannioside D exhibits antioxidant and anti-inflammatory activities. It is used as a reference standard for analytical purposes. Quantitative in vitro activity data, such as IC50 values for antioxidant or anti-inflammatory effects, is not detailed in the publicly available sources.
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| ln Vivo |
In vivo activity data for Rehmannioside D is limited in publicly available literature. As a compound with antioxidant and anti-inflammatory properties in vitro, it is hypothesized to exhibit similar effects in vivo. However, specific in vivo efficacy studies, including pharmacokinetic and pharmacodynamic parameters, have not been detailed in the available sources. It is used as a quality control marker for Rehmannia root in traditional Chinese medicine.
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| Enzyme Assay |
For in vitro cell-free assays, the antioxidant activity of Rehmannioside D can be measured using DPPH or ABTS radical scavenging assays. Its anti-inflammatory activity can be assessed using enzyme inhibition assays for inflammatory mediators.
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| Cell Assay |
For in vitro cellular assays, the antioxidant activity of Rehmannioside D can be assessed in cell lines by measuring its ability to reduce oxidative stress. Its anti-inflammatory activity can be assessed in immune cells by measuring the production of inflammatory cytokines.
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| Animal Protocol |
For in vivo studies, Rehmannioside D could be administered orally in animal models of oxidative stress or inflammation. Endpoints include antioxidant enzyme levels, inflammatory biomarker analysis, and tissue histopathology.
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| ADME/Pharmacokinetics |
Rehmannioside D (CAS 81720-08-3) has a molecular formula of C27H42O20 and a molecular weight of 686.61 g/mol. IUPAC name: [(1S,7aα)-1α-(β-D-Glucopyranosyloxy)-5,7a-dihydro-5α-hydroxy-7-(hydroxymethyl)cyclopenta[c]pyran-4aα(1H)-yl]2-O-β-D-glucopyranosyl-β-D-glucopyranoside. Appearance: yellow solid. Solubility: DMSO and other organic solvents. Storage: typical for glycosides (desiccated, protected from light, -20°C). Purity: 94.2% (as a reference standard).
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| Toxicity/Toxicokinetics |
No detailed toxicity data is publicly available. As a natural iridoid glycoside from Rehmannia glutinosa, it is generally considered to have low toxicity, but standard toxicological studies would be required for drug development.
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| Additional Infomation |
Ramaniside D is an iridoid monoterpene compound and also a glycoside. It has been reported to be found in Rehmannia glutinosa, Quindina maca, and Plantago asiatica, and relevant data are available for reference.
Rehmannioside D is a research-grade compound and reference standard, not a drug. It is used as a quality control standard for the Chinese Pharmacopoeia for Rehmannia and prepared Rehmannia root. It exhibits antioxidant, anti-inflammatory, and moisture-supporting properties. No clinical trials have been reported. |
| Molecular Formula |
C27H42O20
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|---|---|
| Molecular Weight |
686.6104
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| Exact Mass |
686.226
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| CAS # |
81720-08-3
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| PubChem CID |
92044472
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| Appearance |
White to off-white solid powder
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| Density |
1.8±0.1 g/cm3
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| Boiling Point |
1052.4±65.0 °C at 760 mmHg
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| Flash Point |
590.3±34.3 °C
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| Vapour Pressure |
0.0±0.6 mmHg at 25°C
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| Index of Refraction |
1.707
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| LogP |
-5.78
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| Hydrogen Bond Donor Count |
13
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| Hydrogen Bond Acceptor Count |
20
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
47
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| Complexity |
1110
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| Defined Atom Stereocenter Count |
19
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| SMILES |
C1=CO[C@H]([C@H]2[C@@]1([C@@H](C=C2CO)O)O[C@H]3[C@@H]([C@H]([C@@H]([C@H](O3)CO)O)O)O[C@H]4[C@@H]([C@H]([C@@H]([C@H](O4)CO)O)O)O)O[C@H]5[C@@H]([C@H]([C@@H]([C@H](O5)CO)O)O)O
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| InChi Key |
JQEFRKPLHFKTFL-SNQOEBIKSA-N
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| InChi Code |
InChI=1S/C27H42O20/c28-4-8-3-12(32)27(1-2-41-23(13(8)27)46-25-21(40)18(37)15(34)10(6-30)43-25)47-26-22(19(38)16(35)11(7-31)44-26)45-24-20(39)17(36)14(33)9(5-29)42-24/h1-3,9-26,28-40H,4-7H2/t9-,10-,11-,12-,13+,14-,15-,16-,17+,18+,19+,20-,21-,22-,23+,24+,25+,26+,27-/m1/s1
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| Chemical Name |
(2S,3R,4S,5S,6R)-2-[(2S,3R,4S,5S,6R)-2-[[(1S,4aS,5R,7aR)-5-hydroxy-7-(hydroxymethyl)-1-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-5,7a-dihydro-1H-cyclopenta[c]pyran-4a-yl]oxy]-4,5-dihydroxy-6-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~125 mg/mL (~182.05 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4564 mL | 7.2822 mL | 14.5643 mL | |
| 5 mM | 0.2913 mL | 1.4564 mL | 2.9129 mL | |
| 10 mM | 0.1456 mL | 0.7282 mL | 1.4564 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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