| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg | |||
| Other Sizes |
| Targets |
Rbin-2's primary target is Midasin (Mdn1), an enzyme belonging to the AAA+ (ATPases associated with diverse cellular activities) family. By directly targeting the AAA+ ATPase activity of Midasin, it inhibits eukaryotic ribosome production. This makes it a potent probe for studying eukaryotic ribosome assembly processes.
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| ln Vitro |
In vitro, Rbin-2 inhibits the growth of fission yeast strains with a GI50 of 14 nM. It also inhibits Mdn1 ATPase activity with an apparent EC50 of approximately 0.3 µM. Its activity is characterized by its high potency and selectivity for its target.
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| ln Vivo |
Specific in vivo activity data for Rbin-2 is not detailed in the search results. As a research tool, its primary application is in in vitro and cell-based studies to elucidate the function of Midasin in ribosome biogenesis.
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| Enzyme Assay |
The ATPase activity of Midasin can be measured in a cell-free system. In this assay, the purified Midasin protein is incubated with ATP in the presence of varying concentrations of Rbin-2. The amount of inorganic phosphate (Pi) released from ATP hydrolysis is measured, typically using a colorimetric assay.
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| Cell Assay |
For cellular assays, fission yeast strains or other eukaryotic cell lines are used. Cells are treated with Rbin-2, and its effect on cell growth is assessed. Its impact on ribosome biogenesis can be evaluated by monitoring the levels of ribosomal RNA or the formation of ribosomal subunits.
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| Animal Protocol |
In vivo studies for Rbin-2 are not described in the search results. It is a research compound used to study ribosome biogenesis in vitro and in cell-based models.
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| ADME/Pharmacokinetics |
Rbin-2 is soluble in DMSO at 1 mg/mL (warmed). It is stable as a powder at -20°C for 3 years.
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| Toxicity/Toxicokinetics |
Specific toxicity data for Rbin-2 is not available in the search results. As a research chemical, standard safety precautions should be taken.
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| References | |
| Additional Infomation |
Rbin-2 is a highly specific research tool for studying the role of Midasin (Mdn1) in eukaryotic ribosome biogenesis. Its development as a potent and selective inhibitor has provided a valuable probe for understanding this essential cellular process. It is not a pharmaceutical drug and is used exclusively for research purposes.
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| Molecular Formula |
C13H11BRN4S
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|---|---|
| Molecular Weight |
335.222239732742
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| Exact Mass |
333.988
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| Elemental Analysis |
C, 46.58; H, 3.31; Br, 23.84; N, 16.71; S, 9.56
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| CAS # |
2032282-97-4
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| Related CAS # |
2032282-97-4;
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| PubChem CID |
129909865
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.6±0.1 g/cm3
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| Boiling Point |
524.3±60.0 °C at 760 mmHg
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| Flash Point |
270.9±32.9 °C
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| Vapour Pressure |
0.0±1.3 mmHg at 25°C
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| Index of Refraction |
1.750
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| LogP |
4.05
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
19
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| Complexity |
354
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| Defined Atom Stereocenter Count |
0
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| SMILES |
N1C2=C(C=CC(Br)=C2)C2=NN=C(SCC(C)=C)N=C12
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| InChi Key |
OVDAOBDZJJZJMY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C13H11BrN4S/c1-7(2)6-19-13-16-12-11(17-18-13)9-4-3-8(14)5-10(9)15-12/h3-5H,1,6H2,2H3,(H,15,16,18)
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| Chemical Name |
7-bromo-3-(2-methylprop-2-enylsulfanyl)-5H-[1,2,4]triazino[5,6-b]indole
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| Synonyms |
Rbin-2
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~8.33 mg/mL (~24.85 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9831 mL | 14.9156 mL | 29.8312 mL | |
| 5 mM | 0.5966 mL | 2.9831 mL | 5.9662 mL | |
| 10 mM | 0.2983 mL | 1.4916 mL | 2.9831 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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