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| 500mg |
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Purity: ≥98%
Pyronine is a Xanthene analog used as a dye and a bacterial and biological stain. It is a red band that appears during electrophoresis and is a cationic dye that intercalates RNA. It has also been demonstrated to build up in living cells' mitochondria. Pyronin Y is a fluorescent differential stain for nucleic acids that can be used on paraffin tissue sections in conjunction with Nancy-520 or Methyl Green.
| ln Vitro |
In order to estimate formalin-fixed EL4 blank tumors, cellular enzyme-dispersed R3327-RNA content per cell in G-transporting adenocarcinoma cells, mouse splenocytes stimulated with concanavalin, and human peripheral blood stimulated with phytohemagglutinin, pyronin Y forms a fluorophore complex with double-stranded sequences, particularly RNA [1]. Give an explanation of the Jionin Y-based fluorescent staining technique. Human reticulocytes have been treated with this method to dye their RNA in preparation for sorting and process analysis [2]. This dye gathers in the mitochondria of live cells. Jiaoning Y forms additional mitochondria in the mitochondria of cultivated cells when it is almost entirely localized there at 1.7 to 3.3 μM! Rhodamine 123 probe. PY is not harmful at this dose, but it will stop cell division and induce apoptosis [3]. For a considerable amount of time, paraffin tissue sections have been differentially stained using pyronin Y in conjunction with other dyes like methyl green. Cell nuclei appeared non-fluorescent in methyl green-pyronin Y stained sections, whereas red blood cells, elastic fibers, amygdala acinar cell zymogen granules, liver cell surface membranes, and renal tubular cells all displayed intense green and/or red fluorescence[4]. Double-stranded RNA (dsRNA) such as messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA) are bound by pyrin Y [5].
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| References |
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| Additional Infomation |
Pyronin Y is an organic chloride salt having 6-(dimethylamino)-N,N-dimethyl-3H-xanthen-3-iminium as the cation. Used with methyl green to selectively demonstrate RNA (red) in contrast to DNA (green) with the Unna-Pappenheim method. It has a role as a histological dye. It is an iminium salt and an organic chloride salt. It contains a pyronin Y cation.
Xanthene dye used as a bacterial and biological stain. Synonyms: Pyronin; Pyronine G; Pyronine Y. Use also for Pyronine B. which is diethyl-rather than dimethylamino-. |
| Molecular Formula |
C17H19CLN2O
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|---|---|
| Molecular Weight |
302.7986
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| Exact Mass |
302.118
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| Elemental Analysis |
C, 67.43; H, 6.32; Cl, 11.71; N, 9.25; O, 5.28
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| CAS # |
92-32-0
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| PubChem CID |
7085
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| Appearance |
Light brown to black solid powder
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| Melting Point |
250-260ºC(lit.)
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
21
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| Complexity |
480
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[Cl-].[O+]1C2C(=CC=C(N(C)C)C=2)C=C2C=1C=C(N(C)C)C=C2
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| InChi Key |
INCIMLINXXICKS-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
|
| Chemical Name |
[6-(dimethylamino)xanthen-3-ylidene]-dimethylazanium;chloride
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| Synonyms |
Pyronin Y; Pyronine
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| HS Tariff Code |
2934.99.03.00
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~82.56 mM)
H2O : ~4 mg/mL (~13.21 mM) |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3025 mL | 16.5125 mL | 33.0251 mL | |
| 5 mM | 0.6605 mL | 3.3025 mL | 6.6050 mL | |
| 10 mM | 0.3303 mL | 1.6513 mL | 3.3025 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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