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| 1mg |
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| Targets |
Pyridoxamine 5′-phosphate is a cofactor for various enzymes, particularly transaminases. It acts as a coenzyme in the transfer of amino groups. As a vitamin B6 derivative, it is involved in amino acid metabolism, neurotransmitter synthesis, and other metabolic pathways. It is a substrate for pyridoxamine-5′-phosphate phosphatase and is used in the enzymatic synthesis of cytidine diphosphate-4-keto-3,6-dideoxyglucose.
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| ln Vitro |
Pyridoxal 5'-phosphate can be changed into pyridoxamine 5'-phosphate. In the presence of (S)-α-methylbenzylamine (MBA) as the amine donor, purified His-tagged omega-TA CV2025 from Chromobacterium violaceum can fully convert pyridoxal 5'-phosphate to pyridoxamine 5. The acid phosphoric.
In vitro, pyridoxamine 5′-phosphate is used as a cofactor in enzymatic assays to study transaminase activity and other vitamin B6-dependent reactions. It is a substrate for pyridoxamine-5′-phosphate phosphatase activity assays. It can be used in the enzymatic synthesis of cytidine diphosphate-4-keto-3,6-dideoxyglucose. |
| ln Vivo |
In vivo, pyridoxamine 5′-phosphate plays a crucial role as a coenzyme in numerous metabolic processes, including amino acid and neurotransmitter metabolism. It is essential for normal neurological function and may contribute to metabolic health.
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| Enzyme Assay |
In vitro non-cell-based assays for pyridoxamine 5′-phosphate involve its use as a cofactor in enzymatic reactions. The compound is added to reaction mixtures containing the apoenzyme and substrate, and the enzymatic activity is measured by monitoring the formation of product or the consumption of substrate. For phosphatase assays, the release of phosphate is measured colorimetrically.
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| Cell Assay |
In vitro cell-based assays for pyridoxamine 5′-phosphate are not typical, as it is a cofactor rather than a modulator of cellular function. However, its effects on cells can be studied by measuring the activity of vitamin B6-dependent enzymes in cell lysates. Cells can be treated with the compound, and enzyme activities can be measured.
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| Animal Protocol |
In vivo animal experiments with pyridoxamine 5′-phosphate are not typical, as it is a naturally occurring metabolite. However, studies on vitamin B6 metabolism may involve measuring its levels in tissues or administering it to animals to study its effects on metabolic pathways.
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| ADME/Pharmacokinetics |
Pyridoxamine 5′-phosphate has a molecular weight of 248.17 g/mol and the formula C8H13N2O5P. It is freely soluble in water (583 g/L at 25°C). It is a vitamin B6 phosphate that is the phosphoric ester derivative of pyridoxamine. It is a central metabolite of multiple enzymes.
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| Toxicity/Toxicokinetics |
Pyridoxamine 5′-phosphate is a naturally occurring metabolite and is generally considered to have low toxicity. It is an essential nutrient.
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| References | |
| Additional Infomation |
Pyridoxine 5'-phosphate is a vitamin B6 phosphate derivative, a phosphate derivative of pyridoxine. It is found in humans, Saccharomyces cerevisiae, Escherichia coli, and mice as a metabolite. It belongs to the class of vitamin B6 phosphates, aminoalkylpyridines, monohydroxypyridines, and methylpyridines. Its function is related to pyridoxine. It is the conjugate acid of pyridoxine 5'-phosphate (1-). Pyridoxine 5'-phosphate is a metabolite found or produced by Escherichia coli (K12 strain, MG1655 strain). Pyridoxine phosphate has also been reported in Arabidopsis thaliana, humans, and other organisms with relevant data. Pyridoxine 5'-phosphate is a metabolite found or produced by Saccharomyces cerevisiae.
Pyridoxamine 5′-phosphate is the active form of vitamin B6. It is a central metabolite involved in amino acid metabolism. It is used in research as a cofactor and standard. |
| Molecular Formula |
C8H13N2O5P
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|---|---|
| Molecular Weight |
248.17300
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| Exact Mass |
248.056
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| CAS # |
529-96-4
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| Related CAS # |
951-83-7 (hydrochloride)
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| PubChem CID |
1053
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| Appearance |
White to yellow solid powder
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| Density |
1.559g/cm3
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| Boiling Point |
607.3ºC at 760 mmHg
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| Flash Point |
321.1ºC
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| Index of Refraction |
1.622
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| LogP |
0.863
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
16
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| Complexity |
271
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
ZMJGSOSNSPKHNH-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H13N2O5P/c1-5-8(11)7(2-9)6(3-10-5)4-15-16(12,13)14/h3,11H,2,4,9H2,1H3,(H2,12,13,14)
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| Chemical Name |
[4-(aminomethyl)-5-hydroxy-6-methylpyridin-3-yl]methyl dihydrogen phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.0295 mL | 20.1475 mL | 40.2950 mL | |
| 5 mM | 0.8059 mL | 4.0295 mL | 8.0590 mL | |
| 10 mM | 0.4029 mL | 2.0147 mL | 4.0295 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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