| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| ln Vitro |
Antiplatelet Activity: Pseudojervine (compound 10) exhibited feeble inhibition activity against platelet aggregation in rabbit platelet-rich plasma. When tested at concentrations of 250, 500, and 1000 μM against aggregation induced by arachidonic acid (AA), adenosine diphosphate (ADP), and platelet-activating factor (PAF), most inhibition rates were lower than 50%. The highest inhibition observed was 40.1% against AA-induced aggregation at 1000 μM. The compound showed little to no inhibition against ADP and PAF-induced aggregation at all tested concentrations. [1]
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| References | |
| Additional Infomation |
Pseudo-Zelvin is a steroidal saponin. It has been reported to exist in Veratrum talitum, Veratrum sylvestris, and other organisms with relevant data.
Background: Pseudojervine is a steroidal alkaloid isolated from the rhizomes of Veratrum dahuricum, a plant used in traditional Chinese medicine. It belongs to the jervetram group of alkaloids, which also includes cyclopamine, veratramine, jervine, and veratrosine. Its structure was confirmed by comparing spectroscopic data with literature. The purity of the isolated pseudojervine used in this study ranged from 95.0% to 96.6%. [1] Classification and Selectivity: The study notes that jervetram alkaloids (including pseudojervine) and cevratrum alkaloids showed obvious selectivity in their bioactivities. Pseudojervine, as a jervetram alkaloid, demonstrated very weak antiplatelet activity compared to the cevratrum alkaloids, which were identified as the main active constituents responsible for antiplatelet effects. [1] Lack of Activity: Unlike cyclopamine and some other alkaloids from the same plant, pseudojervine was not reported to have significant antitumor activity or Hedgehog pathway inhibition in this study. Its primary evaluation was in the antiplatelet assay, where it showed minimal effects. [1] |
| Molecular Formula |
C33H49NO8
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|---|---|
| Molecular Weight |
587.75
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| Exact Mass |
587.346
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| CAS # |
36069-05-3
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| PubChem CID |
16398499
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.1485 g/cm3
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| Boiling Point |
643.44℃ at 760 mmHg
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| Melting Point |
300-301℃
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| LogP |
2.333
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
42
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| Complexity |
1170
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| Defined Atom Stereocenter Count |
15
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| SMILES |
O1[C@]2([H])C([H])([H])[C@]([H])(C([H])([H])[H])C([H])([H])N([H])[C@@]2([H])[C@@]([H])(C([H])([H])[H])[C@@]21C(C([H])([H])[H])=C1C([C@]3([H])[C@@]4(C([H])([H])[H])C([H])([H])C([H])([H])[C@@]([H])(C([H])([H])C4=C([H])C([H])([H])[C@@]3([H])[C@]1([H])C([H])([H])C2([H])[H])O[C@@]1([H])[C@@]([H])([C@]([H])([C@@]([H])([C@@]([H])(C([H])([H])O[H])O1)O[H])O[H])O[H])=O
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| InChi Key |
HYDDDNUKNMMWBD-VPLHBGEQSA-N
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| InChi Code |
InChI=1S/C33H49NO8/c1-15-11-22-26(34-13-15)17(3)33(42-22)10-8-20-21-6-5-18-12-19(40-31-30(39)29(38)27(36)23(14-35)41-31)7-9-32(18,4)25(21)28(37)24(20)16(33)2/h5,15,17,19-23,25-27,29-31,34-36,38-39H,6-14H2,1-4H3/t15-,17+,19-,20-,21-,22+,23+,25+,26-,27+,29-,30+,31+,32-,33-/m0/s1
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| Chemical Name |
(3S,3'R,3'aS,6'S,6aS,6bS,7'aR,9R,11aS,11bR)-3',6',10,11b-tetramethyl-3-[(2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyspiro[1,2,3,4,6,6a,6b,7,8,11a-decahydrobenzo[a]fluorene-9,2'-3a,4,5,6,7,7a-hexahydro-3H-furo[3,2-b]pyridine]-11-one
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| Synonyms |
Jervin-3-glucoside BRN-0072750 BRN0072750BRN 0072750 Pseudojervine Pseudojervin
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7014 mL | 8.5070 mL | 17.0140 mL | |
| 5 mM | 0.3403 mL | 1.7014 mL | 3.4028 mL | |
| 10 mM | 0.1701 mL | 0.8507 mL | 1.7014 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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