| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 500mg | |||
| 1g | |||
| Other Sizes |
| Targets |
1-Pyrenedecanoic acid targets lipid membranes and lipid-water interfaces. As a fluorescent lipid analog, it partitions into phospholipid bilayers and lipid droplets, allowing visualization of membrane dynamics. It can be formed into micelles with other lipids (triglycerides, phospholipids). Its fluorescence properties are sensitive to the local environment, making it useful for studying membrane properties.
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|---|---|
| ln Vitro |
In vitro, 1-Pyrenedecanoic acid is used as a fluorescent probe to measure membrane fluidity and lipid organization. Its pyrene fluorophore exhibits excimer formation at high local concentrations, providing information about lipid packing and lateral diffusion. It is also used as a substrate in lipase enzyme assays, where enzymatic cleavage releases the fluorescent fatty acid.
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| ln Vivo |
In vivo activity data for 1-Pyrenedecanoic acid are not applicable as it is a fluorescent probe used in biochemical assays. Its primary applications are in in vitro systems for studying lipid membranes and enzyme activity. It is not intended for therapeutic use.
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| Enzyme Assay |
The non-cellular assay for 1-Pyrenedecanoic acid involves incorporating the probe into lipid vesicles or micelles and measuring fluorescence. Excimer formation (emission at ~470 nm) versus monomer fluorescence (~380 nm) is used to assess lipid packing and lateral diffusion. Lipase activity is measured by monitoring the increase in fluorescence upon cleavage of the ester bond.
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| Cell Assay |
In vitro cell-based assays for 1-Pyrenedecanoic acid involve incubating cells with the fluorescent probe, which partitions into cellular membranes. Fluorescence microscopy or flow cytometry is used to visualize and quantify membrane labeling. The probe's sensitivity to membrane fluidity allows assessment of membrane dynamics in response to various stimuli or treatments.
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| Animal Protocol |
In vivo animal study protocols for 1-Pyrenedecanoic acid are not typical as it is a research probe rather than a therapeutic agent. However, it can be administered to animals for imaging studies, with tissue distribution assessed by fluorescence imaging. Standard protocols for fluorescent probe administration and imaging would apply.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 1-Pyrenedecanoic acid are not characterized as it is a research probe. It is soluble in DMSO and other organic solvents. The compound is light-sensitive. It is not intended for therapeutic use, and PK data are not available.
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| Toxicity/Toxicokinetics |
Toxicological data for 1-Pyrenedecanoic acid are not extensively reported. As a research compound, it is intended for laboratory use only. Standard safety precautions should be observed during handling. The compound is not approved for human use.
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| Additional Infomation |
1-Pyrenedecanoic acid is a fluorescent lipid analog used for studying membrane dynamics and lipase activity. It incorporates into membranes and lipid bilayers and can form micelles. The compound is light-sensitive. It is not a therapeutic drug and has no clinical trial or marketing authorization status.
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| Molecular Formula |
C26H28O2
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|---|---|
| Molecular Weight |
372.50
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| CAS # |
64701-47-9
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| Appearance |
Typically exists as solids at room temperature
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| Density |
1.158 g/cm3
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| Boiling Point |
576.4ºC at 760 mmHg
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| Flash Point |
472.6ºC
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| Index of Refraction |
1.673
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| LogP |
7.332
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| SMILES |
C(CCCCC1=C2C=CC3=CC=CC4=C3C2=C(C=C1)C=C4)CCCCC(=O)O
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| Synonyms |
1-Pyrenedecanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6846 mL | 13.4228 mL | 26.8456 mL | |
| 5 mM | 0.5369 mL | 2.6846 mL | 5.3691 mL | |
| 10 mM | 0.2685 mL | 1.3423 mL | 2.6846 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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