| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| Targets |
Deamino-NAD sodium serves as a substrate for rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GPDH) in glycolysis, with enzyme kinetic parameters of Km = 2300 pm and Kd = 112 pm. These parameters indicate that this compound exhibits certain affinity for interaction with GPDH and can serve as a tool molecule for studying the catalytic mechanism of this enzyme.
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|---|---|
| ln Vitro |
Deamino-NAD sodium functions as a substrate for rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GPDH) in the glycolytic pathway. Through its binding to GPDH, it participates in the classical glycolytic metabolic pathway. The determined kinetic parameters include a Michaelis constant (Km) of 2300 pm and a dissociation constant (Kd) of 112 pm. These values provide quantitative insights into the interaction between this compound and GPDH.
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| Enzyme Assay |
Classical enzyme kinetic methods are typically used to study the interaction of Deamino-NAD sodium with rabbit muscle GPDH. The general procedure involves incubating purified rabbit muscle GPDH with varying concentrations of Deamino-NAD sodium in an appropriate reaction buffer, followed by monitoring NADH production or consumption to determine the enzymatic reaction rate. The Michaelis constant (Km = 2300 pm) and dissociation constant (Kd = 112 pm) are calculated using Lineweaver-Burk double reciprocal plotting or nonlinear regression analysis to assess the affinity and catalytic efficiency of this compound as a substrate.
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| References |
| Molecular Formula |
C21H25N6NAO15P2
|
|---|---|
| Molecular Weight |
686.39
|
| CAS # |
104809-38-3
|
| PubChem CID |
136663121
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| Appearance |
Typically exists as solids at room temperature
|
| LogP |
0
|
| Hydrogen Bond Donor Count |
6
|
| Hydrogen Bond Acceptor Count |
17
|
| Rotatable Bond Count |
11
|
| Heavy Atom Count |
45
|
| Complexity |
1200
|
| Defined Atom Stereocenter Count |
8
|
| SMILES |
[NaH].NC(C1C=CC=[N+](C2OC(COP(OP(OCC3OC(N4C=NC5C(N=CNC4=5)=O)C(O)C3O)(O)=O)(=O)[O-])C(O)C2O)C=1)=O
|
| Synonyms |
Deamino-NAD (sodium); Deamino-NAD sodium;
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4569 mL | 7.2845 mL | 14.5690 mL | |
| 5 mM | 0.2914 mL | 1.4569 mL | 2.9138 mL | |
| 10 mM | 0.1457 mL | 0.7284 mL | 1.4569 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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