| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
IC50: 2.4±0.6 μM (PI3K γ), 3.0±0.0 μM (PI3K δ), 5.4±0.3 μM (PI3K β)[1]
Quercetin-d3 hydrate targets PI3K (phosphoinositide 3-kinase) isoforms, with IC50 values of 2.4 uM for PI3Kgamma, 3.0 uM for PI3Kdelta, and 5.4 uM for PI3Kbeta. It also stimulates recombinant SIRT1, a NAD+-dependent deacetylase involved in aging and metabolism. As a deuterated standard, it is chemically identical to quercetin but has a different mass, allowing for precise quantification by mass spectrometry. |
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| ln Vitro |
Quercetin hydrate is a phytochemical or plant chemical used as an ingredient in supplements, beverages, or foods. In several studies, it may have anti-inflammatory and antioxidant properties and is being investigated for a wide range of potential health benefits. Quercetin hydrate is a PI3K inhibitor with an IC50 of 2.4-5.4 μM. Quercetin hydrate strongly inhibits PI3K and Src kinases, mildly inhibits Akt1/2, and slightly affects PKC, p38, and ERK1/2[1]. Quercetin hydrate inhibits TNF-induced LDH% release, EC-dependent neutrophil adhesion to bovine pulmonary artery endothelial cells (BPAEC), and BPAEC DNA synthesis and proliferation[2].
Quercetin-d3 hydrate is a PI3K inhibitor with IC50 values of 2.4 uM, 3.0 uM, and 5.4 uM for PI3Kgamma, PI3Kdelta, and PI3Kbeta, respectively. It also stimulates recombinant SIRT1 activity. However, as a deuterated internal standard, it is used at tracer concentrations (ng/mL) for analytical purposes, and its biological activity is not exploited in standard assays. The deuterium label has minimal impact on its pharmacological properties. |
| ln Vivo |
The combination of Quercetin hydrate (75 mg/kg) and 2-methoxyestradiol enhanced the inhibition of human prostate cancer LNCaP and PC-3 cell xenograft tumor growth [3].
Specific in vivo activity data for Quercetin-d3 hydrate is not provided. As an internal standard, it is not administered therapeutically. The non-deuterated parent compound, quercetin, is known for its antioxidant, anti-inflammatory, and anti-cancer activities. It is used in metabolic studies to quantify quercetin levels in biological samples by LC-MS/MS. |
| Enzyme Assay |
Not applicable. Quercetin-d3 hydrate is an analytical internal standard used for LC-MS/MS quantification, not for cell-free binding assays. For PI3K inhibition studies, a fluorescence polarization (FP) assay can be performed using the non-deuterated compound. Procedure: Recombinant PI3K isoforms are incubated with a fluorescent PIP2 substrate and varying concentrations of quercetin. The IC50 is calculated from the dose-response curve.
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| Cell Assay |
Not applicable. Quercetin-d3 hydrate is used as an internal standard in LC-MS/MS analysis of cell lysates or tissue homogenates, not for studying biological activity on cells. For analytical purposes, cells are lysed, and the deuterated standard is added to the lysate at a known concentration. Proteins are precipitated with acetonitrile, and the supernatant is analyzed by LC-MS/MS. The peak area ratio of quercetin to the internal standard is used to calculate the concentration of endogenous quercetin in the sample.
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| Animal Protocol |
Quercetin-d3 hydrate is used as an internal standard in animal pharmacokinetic studies. Procedure: Mice are administered quercetin (non-deuterated) orally or intraperitoneally. At various time points, blood or tissue samples are collected. A known amount of Quercetin-d3 hydrate (internal standard) is added to each sample. Samples are extracted and analyzed by LC-MS/MS. The concentration of quercetin in the samples is calculated by comparing the peak area ratio of quercetin to the internal standard to a calibration curve prepared with known concentrations.
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| ADME/Pharmacokinetics |
Quercetin-d3 hydrate is an analytical internal standard and is not used as a drug. The pharmacokinetic profile of quercetin is known: it has poor oral bioavailability (approximately 2-5%) due to extensive metabolism and efflux. Its plasma half-life is short (2-4 hours). The deuterated standard has identical PK properties and is used to accurately quantify these parameters.
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| Toxicity/Toxicokinetics |
Specific toxicology data for Quercetin-d3 hydrate is not provided. As a deuterated flavonoid used at trace levels as an internal standard, it poses no toxicological risk. The parent compound quercetin is generally recognized as safe (GRAS) at dietary levels. Standard safety precautions for handling organic compounds should be followed.
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| References |
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| Additional Infomation |
Quercetin-d3 hydrate is a deuterium-labeled internal standard used for the quantification of quercetin by GC- or LC-MS. Quercetin is an abundant flavonoid found in fruits, vegetables, and teas, and is known for its antioxidant, anti-inflammatory, anti-cancer, and cardioprotective properties. The deuterated version has the same chemical properties but a different mass (+3 Da), allowing for precise and accurate quantification in biological samples. This product is for research use only and is not an approved drug.
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| Molecular Formula |
C15H9D3O8
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|---|---|
| Molecular Weight |
323.27
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| Appearance |
Typically exists as solids at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~30 mg/mL (~92.80 mM; with heating and sonication)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0934 mL | 15.4669 mL | 30.9339 mL | |
| 5 mM | 0.6187 mL | 3.0934 mL | 6.1868 mL | |
| 10 mM | 0.3093 mL | 1.5467 mL | 3.0934 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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