| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Not applicable. It is used as an analytical internal standard. (Unlabeled guanosine targets HSV).
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|---|---|
| ln Vitro |
No specific in vitro biological activity is attributed to the isotope-labeled form itself, as it is a tracer compound. The unlabeled guanosine has anti-HSV activity. This labeled compound is used in research as an internal standard to study the mechanisms of antiviral agents, providing insights into drug interactions and effectiveness. It can be used in research to boost the effectiveness of antiviral therapies and minimize the emergence of resistant viral strains when used in conjunction with other compounds.
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| ln Vivo |
No specific in vivo biological activity is attributed to the isotope-labeled form itself. As a tracer compound, the labeled nucleoside can be tracked in vivo to study nucleotide metabolism, pharmacokinetics of guanosine derivatives, and antiviral mechanisms. It is utilized in studying the mechanisms of antiviral agents by providing stable isotope tracing capabilities.
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| Enzyme Assay |
As this is an internal standard, there is no inherent enzyme/receptor binding activity. For analytical applications, Guanosine-13C10,15N5 is spiked into biological samples (e.g., plasma, cell lysates, tissue homogenates) at known concentrations. The samples undergo protein precipitation, centrifugation, and supernatant collection, followed by LC-MS/MS analysis. Standard curves for guanosine quantification are prepared using unlabeled guanosine with fixed concentrations of the labeled internal standard.
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| Cell Assay |
Cell-based assays employing Guanosine-13C10,15N5 as a tracer involve incubating cells with growth medium containing the labeled compound for defined periods (1-24 hours). After incubation, cells are harvested, and intracellular nucleotides are extracted using cold methanol or perchloric acid. The extracts are analyzed by LC-MS/MS to quantify incorporation of the stable isotope label into guanine nucleotides and RNA, providing information on nucleoside salvage and synthesis pathways.
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| Animal Protocol |
Animal studies using Guanosine-13C10,15N5 as a tracer involve intravenous or oral administration to rodents (mice or rats) at predetermined doses. Blood samples are collected at various time points post-dosing via tail vein or cardiac puncture. Plasma is separated and processed for LC-MS/MS analysis of labeled compound concentration. Tissues (liver, kidney, brain) may be harvested and analyzed to track tissue distribution and metabolism of guanosine-derived compounds in vivo.
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| ADME/Pharmacokinetics |
No specific PK data exists for the labeled compound that differs from unlabeled guanosine. Guanosine itself is a naturally occurring purine nucleoside with rapid metabolism and clearance. Guanosine-13C10,15N5 is soluble in DMSO and water, with molecular weight 298.13 and purity typically >98%. For in vitro experiments, it can be dissolved in DMSO (5-10 mM) or in saline. In vivo formulations may be prepared in saline or isotonic buffer. Storage: powder at -20degC (stable for 3 years), in solvent at -80degC (6 months).
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| Toxicity/Toxicokinetics |
No toxicology data is available for this isotope-labeled compound. Guanosine, the unlabeled parent compound, is a naturally occurring nucleoside with low inherent toxicity. As a stable isotope-labeled research chemical (not radioactive), Guanosine-13C10,15N5 is considered non-hazardous in terms of radiation exposure. However, standard laboratory chemical safety precautions should be followed. The compound is for research use only and not for human or veterinary use.
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| References | |
| Additional Infomation |
Guanosine-13C10,15N5 is a stable isotope-labeled (13C10 and 15N5) nucleoside used as an internal standard for mass spectrometry-based quantification. It has applications in antiviral research, particularly for studying HSV (Herpes simplex virus) infection and nucleotide metabolism. It is also used in pharmacokinetic studies and metabolic flux analysis. The compound is strictly for laboratory research use, not for clinical or therapeutic applications, and is not approved as a drug. It is not intended for human consumption.
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| Molecular Formula |
13C10H1315N5O5
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|---|---|
| Molecular Weight |
298.13
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| CAS # |
202406-81-3
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| Appearance |
White to off-white solid powder
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| Synonyms |
DL-Guanosine-13C10,15N5;Vernine-13C10,15N5
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3542 mL | 16.7712 mL | 33.5424 mL | |
| 5 mM | 0.6708 mL | 3.3542 mL | 6.7085 mL | |
| 10 mM | 0.3354 mL | 1.6771 mL | 3.3542 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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