| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Not applicable; this is a fluorescent dye and labeling reagent, not a biologically active drug with a defined molecular target.
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| ln Vitro |
No direct biological activity is attributed to Sulfo-Cy5 carboxylic acid. In vitro, it can be chemically activated to a reactive NHS ester (using EDC and NHS), which then conjugates to primary amines on antibodies, proteins, or other biomolecules. The labeled conjugate can then be used as a fluorescent probe for cell staining, flow cytometry, or immunofluorescence assays. The dye itself does not inhibit enzymes, bind to receptors, or modulate signaling pathways. It is a passive fluorescent tag. As an "inactive" carboxylic acid, it is often used as a negative control in labeling experiments.
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| ln Vivo |
The free dye is not administered to animals for therapeutic purposes. Sulfo-Cy5 carboxylic acid can be conjugated to targeting vectors (e.g., antibodies, peptides, nanoparticles) for in vivo near-infrared fluorescence imaging in animal models. The conjugate is injected intravenously into mice, and the NIR fluorescence (ex/em 648/670 nm) is used to track biodistribution, tumor accumulation, or drug delivery. The dye itself has no intrinsic pharmacodynamic activity. No in vivo efficacy studies are conducted with the unconjugated dye. The carboxylic acid form is typically used as an intermediate for the synthesis of activated esters or other derivatives.
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| Enzyme Assay |
The free dye does not bind to any specific receptor or enzyme. Its carboxylic acid group can be activated for conjugation, but it does not engage in biological target binding. For quality control, the absorption spectrum is measured in PBS or water to confirm the peak at 648 nm. The dye can be analyzed by HPLC-MS. If activation is performed, the formation of the NHS ester can be monitored by TLC or LC-MS. No classical receptor binding assays (e.g., radioligand displacement, enzyme inhibition) are performed for this compound, as it is a labeling reagent, not an active pharmaceutical ingredient.
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| Cell Assay |
The dye is not used directly on cells; it must first be activated and conjugated to a targeting molecule. To activate, dissolve Sulfo-Cy5 carboxylic acid in MES buffer (pH 5.5, 10 mM) to a concentration of 10 mM. Add a 10-fold molar excess of EDC and a 20-fold excess of sulfo-NHS. Incubate at room temperature for 15 minutes. The resulting NHS ester can be used without purification. Then add the activated dye to a protein (e.g., 1 mg/mL antibody in PBS, pH 7.4) at a 10-20 fold molar excess. Incubate for 2 hours at room temperature. Purify by gel filtration or dialysis. Add the labeled antibody (1-20 ug/mL) to cells in culture for 30-60 min at 37degC, wash, and analyze by flow cytometry or fluorescence microscopy.
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| Animal Protocol |
The dye is not administered directly; it is first conjugated to a targeting vector. For imaging studies, the conjugate (e.g., antibody-dye) is injected intravenously (tail vein) into tumor-bearing mice at a dose of 0.5-5 mg/kg (based on conjugate). At specified time points (e.g., 1, 6, 24, 48, 72 hours), mice are anesthetized and imaged using an in vivo imaging system with a Cy5 filter set (excitation 640-660 nm, emission 670-720 nm). Fluorescence intensity in tumors, major organs, and blood is quantified. Mice are euthanized, and tissues are collected for ex vivo imaging. All procedures require IACUC approval. The unconjugated dye is not used in animals.
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| ADME/Pharmacokinetics |
No formal PK data exists for the free dye. The sulfonate groups (2) make the dye highly water-soluble and prevent membrane permeability; it is expected to be rapidly cleared renally. Plasma half-life of the free dye is very short (<1 hour). For the conjugated form (e.g., antibody), PK is dictated by the carrier. Solubility: Highly soluble in water (>50 mg/mL) and DMSO. The compound is a potassium salt. Store powder at -20degC in a desiccated, light-protected container. Stock solutions in water or DMSO can be stored at -80degC for up to 6 months. Solutions are stable if protected from light. For in vivo use, the conjugate is formulated in sterile PBS or saline.
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| Toxicity/Toxicokinetics |
No specific toxicology data is available for this dye. As a sulfonated cyanine dye, it may cause skin, eye, and respiratory tract irritation. Standard safety precautions should be followed: use in a fume hood, wear gloves, lab coat, and safety goggles. Avoid dust inhalation and direct contact. The compound is not approved for human or veterinary use. It is strictly for research use only. In animal imaging studies with typical conjugate doses (<5 mg/kg), no overt toxicity has been reported, but formal toxicology studies have not been conducted. The free dye should not be injected.
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| References | |
| Additional Infomation |
Sulfo-Cy5 carboxylic acid potassium is a water-soluble, near-infrared cyanine dye used as a labeling reagent and reference standard. Unlike its NHS ester counterpart, the carboxylic acid form is "inactive," meaning it does not directly react with amines; it must be activated by EDC/NHS chemistry to form a reactive intermediate. This makes it ideal for custom conjugation protocols where the researcher controls the activation step. The dye's excitation/emission maxima are 648/670 nm, compatible with standard Cy5 filter sets. It is commonly used in flow cytometry, immunofluorescence, and in vivo imaging. This product is not a drug and has not been approved for any clinical or diagnostic use. It is strictly a research chemical.
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| Molecular Formula |
C32H37KN2O8S2
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|---|---|
| Molecular Weight |
680.87
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| Exact Mass |
680.162
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| CAS # |
1144107-82-3
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| PubChem CID |
74408808
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| Appearance |
Typically exists as solid at room temperature
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
45
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| Complexity |
1400
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[K+].S(C1C=CC2=C(C=1)C(C)(C)C(=CC=CC=CC1C(C)(C)C3C=C(C=CC=3[N+]=1C)S(=O)(=O)[O-])N2CCCCCC(=O)O)(=O)(=O)[O-]
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| InChi Key |
ADTLWAAMVPCPTC-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C32H38N2O8S2.K/c1-31(2)24-20-22(43(37,38)39)15-17-26(24)33(5)28(31)12-8-6-9-13-29-32(3,4)25-21-23(44(40,41)42)16-18-27(25)34(29)19-11-7-10-14-30(35)36;/h6,8-9,12-13,15-18,20-21H,7,10-11,14,19H2,1-5H3,(H2-,35,36,37,38,39,40,41,42);/q;+1/p-1
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| Chemical Name |
potassium;1-(5-carboxypentyl)-3,3-dimethyl-2-[5-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)penta-2,4-dienylidene]indole-5-sulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4687 mL | 7.3435 mL | 14.6871 mL | |
| 5 mM | 0.2937 mL | 1.4687 mL | 2.9374 mL | |
| 10 mM | 0.1469 mL | 0.7344 mL | 1.4687 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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