| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Not applicable; this is a fluorescent labeling reagent, not a biologically active drug with a specific molecular target.
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| ln Vitro |
No direct biological activity is attributed to Cyanine 5 NHS ester itself, as it is an inert fluorescent dye. In vitro, it is used as a fluorescent label to visualize macromolecules such as proteins and antibodies after covalent conjugation. For example, antibodies labeled with this dye can be used to detect specific antigens in cells or tissue sections via immunofluorescence. The dye's fluorescence can be detected using standard Cy5 filter sets (excitation 638 nm, emission 670 nm). The compound has no inherent pharmacological activity in cells and does not interact with receptors or enzymes unless conjugated to a targeting moiety.
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| ln Vivo |
No in vivo biological activity is attributed to the dye itself. Cyanine 5 NHS ester is used in animal research as a fluorescent probe for in vivo imaging after conjugation to targeting vectors such as antibodies, peptides, or nanoparticles. The labeled conjugate can be administered intravenously to mice or rats for near-infrared (NIR) imaging to track tumor accumulation, biodistribution, and pharmacokinetics. The dye's NIR properties allow deeper tissue penetration and reduced background autofluorescence. The dye itself is not active; activity depends entirely on the conjugated biomolecule. This product is not a therapeutic drug and has no intrinsic pharmacodynamic effects.
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| Enzyme Assay |
No enzyme/receptor binding assays are applicable for this NHS ester dye, as its mechanism of action is purely chemical conjugation, not target engagement. For the quality control of the NHS ester, its chemical reactivity can be assessed by reacting it with a model amine compound (e.g., butylamine) in an organic solvent (e.g., DMF or DMSO) at room temperature for 1-2 hours. The reaction progress and formation of the amide-bonded product are monitored by TLC or HPLC. Alternatively, the dye can be conjugated to a standard protein (e.g., BSA), and the labeling efficiency (dye-to-protein ratio) is determined by measuring absorbance at 280 nm and 648 nm using a spectrophotometer.
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| Cell Assay |
Dye-labeling of proteins or antibodies is performed in cell culture media or PBS. A typical protocol involves dissolving the NHS ester dye in anhydrous DMSO to a concentration of 10 mM. The protein to be labeled (e.g., 1 mg/mL in 0.1 M sodium bicarbonate buffer, pH 8.5) is mixed with a 10-20 fold molar excess of the dye solution. The mixture is incubated at room temperature for 1 hour with gentle shaking. Unreacted dye is removed by dialysis, size-exclusion chromatography, or spin filtration. The labeled protein can then be added to cultured cells at desired concentrations (e.g., 1-10 ug/mL) for 30-60 min at 37degC. After washing away unbound label, cells are imaged using a fluorescence microscope or analyzed by flow cytometry.
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| Animal Protocol |
Labeled antibodies or nanoparticles are administered intravenously (e.g., via tail vein) to mice at a dose of 1-10 mg/kg based on the conjugate weight. At various time points post-injection (e.g., 1, 6, 24, 48 hours), animals are anesthetized and whole-body fluorescence images are acquired using an in vivo imaging system equipped with a Cy5 filter set (excitation 640-660 nm, emission 670-720 nm). Blood and major organs (liver, kidney, spleen, lung, heart, tumor) are collected for ex vivo imaging. For passive targeting studies, the unconjugated dye is injected as a control. All animal procedures require IACUC approval.
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| ADME/Pharmacokinetics |
No PK data exists for the NHS ester itself; PK depends on the conjugated molecule. The NHS ester group is hydrolytically unstable in aqueous media; its half-life in PBS (pH 7.4) at 25degC is approximately 1 hour. For the conjugated product (dye-protein), the plasma half-life is determined by the protein carrier. Solubility: Soluble in DCM, DMF, and DMSO (10 mM stock). Store powder at -20degC, desiccated, protected from light. Stock solutions in DMSO can be stored at -80degC for up to 6 months. For in vivo imaging, the labeled conjugate is typically formulated in PBS or sterile saline.
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| Toxicity/Toxicokinetics |
No toxicity data is available for this NHS ester dye alone; toxicity depends on the conjugated molecule. As a chemical reagent, the NHS ester is hydrolytically reactive and can cause skin and eye irritation. The compound is classified as an irritant and may cause respiratory tract irritation if inhaled. Standard laboratory safety precautions must be followed: use in a fume hood, wear chemical-resistant gloves, lab coat, and safety goggles. The compound is not approved for human or veterinary use and is strictly for research purposes only. The dye is not intended for oral consumption or injection in its unconjugated form.
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| References | |
| Additional Infomation |
Cyanine 5 NHS ester tetrafluoroborate is a research-grade fluorescent dye widely used for labeling biomolecules, particularly in flow cytometry, immunofluorescence, and in vivo imaging. It is an amine-reactive N-hydroxysuccinimide (NHS) ester, specifically designed for conjugation to primary amine groups. With excitation/emission maxima of 648/670 nm, it is compatible with standard Cy5 filter sets. Common applications include antibody labeling, protein detection on Western blots, nucleic acid probes, and cell tracing. This product is not a drug and has not undergone clinical trials or received regulatory approval for any therapeutic or diagnostic indication. It is strictly for laboratory use by qualified researchers.
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| Molecular Formula |
C36H42BF4N3O4
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|---|---|
| Molecular Weight |
667.54
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| Exact Mass |
667.32
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| CAS # |
1263093-76-0
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| PubChem CID |
164777226
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| Appearance |
Typically exists as solid at room temperature
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
11
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| Heavy Atom Count |
48
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| Complexity |
1210
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| Defined Atom Stereocenter Count |
0
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| SMILES |
FB(F)F.[F-].O(C(CCCCCN1C2C=CC=CC=2C(C)(C)C1=CC=CC=CC1C(C)(C)C2C=CC=CC=2[N+]=1C)=O)N1C(CCC1=O)=O
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| InChi Key |
LWGOCTDPEVJNTC-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C36H42N3O4.BF4/c1-35(2)26-16-11-13-18-28(26)37(5)30(35)20-8-6-9-21-31-36(3,4)27-17-12-14-19-29(27)38(31)25-15-7-10-22-34(42)43-39-32(40)23-24-33(39)41;2-1(3,4)5/h6,8-9,11-14,16-21H,7,10,15,22-25H2,1-5H3;/q+1;-1
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| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) 6-[(2Z)-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethylindol-1-ium-2-yl)penta-2,4-dienylidene]indol-1-yl]hexanoate;tetrafluoroborate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4980 mL | 7.4902 mL | 14.9804 mL | |
| 5 mM | 0.2996 mL | 1.4980 mL | 2.9961 mL | |
| 10 mM | 0.1498 mL | 0.7490 mL | 1.4980 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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