| Size | Price | Stock | Qty |
|---|---|---|---|
| 1g |
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| Other Sizes |
| Targets |
1,4-Bis(mesitylamino)anthraquinone does not bind to a specific biological receptor or enzyme. It is a hydrophobic dye that physically dissolves or adsorbs to lipophilic cellular components (e.g., membranes, lipid droplets). It has no specific ligand-receptor interaction. It is used to stain samples based on physical solubility and hydrophobicity, rather than affinity-based molecular recognition. It is not a targeted therapeutic agent.
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|---|---|
| ln Vitro |
In vitro, the compound has no intrinsic pharmacological activity (kinase inhibition, receptor agonism). As a dye, it absorbs light strongly in the visible spectrum (blue region). When formulated, it imparts a blue color to materials. It may be used in cell culture to stain lipids, but its biological activity is passive staining. It does not stimulate cell growth or induce apoptosis at typical staining concentrations. It is largely inert.
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| ln Vivo |
The compound is not administered as a drug and has no therapeutic in vivo activity. It may be used in animal studies as a tracer for oil-based formulations or to mark the location of implants. It would likely accumulate in adipose tissue if injected but has no therapeutic effect. No beneficial health effects are associated with ingestion or injection.
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| Enzyme Assay |
Cell‑free assays are related to chemical quality control. HPLC-UV/VIS is used to determine purity by comparing the area of the main peak at a wavelength of interest (e.g., 450-650 nm) to the total area. Thin layer chromatography (TLC) is used to check for the presence of unreacted starting materials (e.g., mesidine or quinizarin) using a suitable solvent system (e.g., toluene/ethyl acetate). These are chemical assays, not biological.
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| Cell Assay |
Cell-based assays are not standard for drug discovery. Researchers may use it as a vital dye to evaluate lipid droplet formation in adipocytes. Cells are treated with differentiation media, then incubated with 1-10 uM of the dye in PBS for 10-30 minutes. After washing, the blue staining is visualized by brightfield or fluorescence microscopy (depending on the excitation spectrum, as some anthraquinones are fluorescent) to visualize neutral lipid content.
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| Animal Protocol |
In vivo animal protocols are not standard for this dye. In environmental toxicology, it might be dosed to fish or rats to study its accumulation (e.g., 100 mg/kg in feed for 28 days). The animals are sacrificed, and the tissues are examined for blue discoloration. The dye persists in the liver and adipose tissue. No therapeutic endpoints are measured. It is used to study the environmental fate of colorants.
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| ADME/Pharmacokinetics |
No pharmacokinetic data is available as it is not a drug. It has very low water solubility and high lipophilicity. If absorbed, it would partition into lipid-rich tissues. It is likely extensively metabolized by the liver (reduction or hydroxylation) and excreted via the bile. The oral absorption is generally limited. Store at room temperature. It is stable to light and heat.
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| Toxicity/Toxicokinetics |
Toxicological data is limited. It is considered a potential irritant to the skin, eyes, and respiratory tract. It may be harmful if swallowed (ingestion of large quantities). It is not listed as a carcinogen by IARC. However, as an anthraquinone dye, there is a theoretical risk of genotoxicity in some analogues, though Solvent Blue 104 is generally considered inert due to its large, hydrophobic structure. Standard laboratory safety precautions for handling fine chemicals should be followed.
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| References | |
| Additional Infomation |
This product is an industrial dye (CI Solvent Blue 104) and a research chemical, not a drug. It is not approved for any medical indication. It is used in solvent-based ink formulations, tinting for gasoline, and as a colorant for plastics. The molecular formula is C32H30N2O2, with a molecular weight of 474.60 g/mol. It is a dark blue or purple solid powder. Purity is typically high (95-99%).
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| Molecular Formula |
C32H30N2O2
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|---|---|
| Molecular Weight |
474.59
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| Exact Mass |
474.23
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| CAS # |
116-75-6
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| PubChem CID |
61042
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
639.5±55.0 °C at 760 mmHg
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| Melting Point |
236.5-237.5 °C
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| Flash Point |
168.9±31.7 °C
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| Vapour Pressure |
0.0±1.9 mmHg at 25°C
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| Index of Refraction |
1.677
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| LogP |
7.25
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
36
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| Complexity |
722
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC1=CC(=C(C(=C1)C)NC2=C3C(=C(C=C2)NC4=C(C=C(C=C4C)C)C)C(=O)C5=CC=CC=C5C3=O)C
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| InChi Key |
DMDRBXCDTZRMHZ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C32H30N2O2/c1-17-13-19(3)29(20(4)14-17)33-25-11-12-26(34-30-21(5)15-18(2)16-22(30)6)28-27(25)31(35)23-9-7-8-10-24(23)32(28)36/h7-16,33-34H,1-6H3
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| Chemical Name |
1,4-bis(2,4,6-trimethylanilino)anthracene-9,10-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1071 mL | 10.5354 mL | 21.0708 mL | |
| 5 mM | 0.4214 mL | 2.1071 mL | 4.2142 mL | |
| 10 mM | 0.2107 mL | 1.0535 mL | 2.1071 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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