| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| Other Sizes |
| Targets |
L-Ribose does not have a specific biological target in mammalian systems, as it is not a naturally occurring sugar. It is not metabolized by conventional pathways and is generally not recognized by most carbohydrate-processing enzymes, which exhibit stereospecificity for D-ribose. However, it can be used as a scaffold for synthesizing L-nucleosides such as L-cytidine and L-thymidine, which then target viral polymerases (e.g., HBV and HCV) by acting as chain terminators.
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| ln Vitro |
L-Ribose itself exhibits no significant intrinsic biological activity or cytotoxicity in standard in vitro assays. It is not a substrate for ribokinase or other carbohydrate-metabolizing enzymes, and it does not inhibit D-ribose metabolism at concentrations up to 10 mM. Its primary utility is as a chiral building block in synthetic organic chemistry. When incorporated into L-nucleoside analogues, the resulting compounds show antiviral activity against hepatitis B virus and hepatitis C virus. No direct cellular activity has been reported for L-ribose alone.
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| ln Vivo |
L-Ribose is not administered as a therapeutic agent and has no reported in vivo pharmacological activity. When ingested, L-ribose is poorly absorbed and likely not metabolized, as mammals lack the necessary enzymes for L-sugar catabolism. It is excreted unchanged in urine. In animal studies, L-ribose has been used as a non-metabolizable control for D-ribose experiments. No therapeutic effects (antiviral, anticancer) are attributed to L-ribose itself; instead, it serves as a precursor for active drug synthesis.
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| Enzyme Assay |
A cell‑free assay for testing L-ribose as a substrate is not applicable due to its inactivity. However, it can be used in enzyme specificity studies. For example, incubate purified ribokinase (0.1 U/mL) with 1 mM L-ribose or D-ribose, 1 mM ATP, 50 mM Tris-HCl pH 7.5, and 10 mM MgCl2 for 30 min at 37degC. Measure ADP production using a coupled pyruvate kinase/lactate dehydrogenase assay (NADH oxidation at 340 nm). D-ribose shows activity; L-ribose shows negligible activity (<5% of D-ribose), confirming enzyme stereospecificity. For purity analysis, use HPLC with refractive index detection.
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| Cell Assay |
L-Ribose is not typically used in cell-based assays for pharmacological activity. It may serve as a negative control in experiments investigating D-ribose metabolism. For cytotoxicity screening, culture HeLa or HepG2 cells in 96-well plates (5,000 cells/well). Treat with L-ribose at concentrations of 0.1-50 mM for 48 hours. Measure cell viability by MTT assay. L-ribose typically shows no cytotoxicity up to 50 mM. It can also be used as a non-metabolizable sugar in transport studies to measure passive diffusion across cell membranes.
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| Animal Protocol |
L-Ribose is not used in standard animal efficacy studies as it lacks intrinsic activity. However, it may be used as a negative control in metabolic tracer studies. For example, administer L-ribose (1 g/kg) by oral gavage to rats and compare with D-ribose. Collect blood at 0, 15, 30, 60, 120, and 240 min. Measure plasma sugar levels by HPLC or enzymatic assays. L-ribose shows minimal absorption and rapid urinary excretion. No therapeutic endpoints are evaluated. This protocol is for studying sugar stereospecificity in absorption and metabolism.
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| ADME/Pharmacokinetics |
No dedicated pharmacokinetic studies are available for L-ribose. Based on physicochemical properties (log P ~-2.5, high water solubility, MW 150.13), it is expected to have very low passive diffusion across cell membranes and is unlikely to be absorbed from the gastrointestinal tract. Any absorbed L-ribose would be rapidly excreted unchanged in urine. It is not metabolized by humans, as mammals lack L-sugar dehydrogenases. For research applications, L-ribose is typically stored as a solid at room temperature or 2-8degC in a dry, dark place. Solutions can be prepared in water at ≥100 mg/mL.
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| Toxicity/Toxicokinetics |
L-Ribose has low toxicity. The acute oral LD50 in rats is expected to be >5000 mg/kg based on its similarity to other monosaccharides. No skin or eye irritation is expected. It is not classified as a hazardous substance. However, as with all fine chemicals, standard laboratory safety precautions should be followed (use of gloves, safety glasses). No carcinogenicity, mutagenicity, or reproductive toxicity data are available, but it is considered safe for laboratory use. Avoid inhalation of dust.
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| References | |
| Additional Infomation |
Aldehyde-L-ribose is an L-ribose, and also an aldehyde-ribose. It is the enantiomer of aldehyde-D-ribose. L-ribose is found in or produced by Escherichia coli (K12 strain, MG1655 strain). It has been reported that alfalfa contains L-ribose, and relevant data are available for reference.
L-Ribose is not a drug and has no approved clinical indications. It is a research-grade specialty chemical used primarily as a building block for the synthesis of L-nucleoside analogues, which have applications as antiviral and anticancer agents. Many L-nucleoside drugs (e.g., telbivudine for hepatitis B) are synthesized from L-ribose. It is also used as an analytical standard in carbohydrate chemistry. L-Ribose is a white to off-white crystalline powder with purity ≥98% (HPLC). Molecular formula: C5H10O5, molecular weight: 150.13 g/mol. Store at room temperature in a cool, dry place protected from light. |
| Molecular Formula |
C5H10O5
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|---|---|
| Molecular Weight |
150.13
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| Exact Mass |
150.052
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| CAS # |
24259-59-4
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| PubChem CID |
90428
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| Appearance |
Solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
415.5±38.0 °C at 760 mmHg
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| Melting Point |
81-84ºC
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| Flash Point |
219.2±23.3 °C
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| Vapour Pressure |
0.0±2.2 mmHg at 25°C
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| Index of Refraction |
1.544
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| LogP |
-2.39
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
10
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| Complexity |
104
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| Defined Atom Stereocenter Count |
3
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| SMILES |
O([H])[C@]([H])([C@@]([H])(C([H])=O)O[H])[C@]([H])(C([H])([H])O[H])O[H]
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| InChi Key |
PYMYPHUHKUWMLA-MROZADKFSA-N
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| InChi Code |
InChI=1S/C5H10O5/c6-1-3(8)5(10)4(9)2-7/h1,3-5,7-10H,2H2/t3-,4+,5-/m1/s1
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| Chemical Name |
(2S,3S,4S)-2,3,4,5-tetrahydroxypentanal
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.6609 mL | 33.3045 mL | 66.6089 mL | |
| 5 mM | 1.3322 mL | 6.6609 mL | 13.3218 mL | |
| 10 mM | 0.6661 mL | 3.3304 mL | 6.6609 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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