| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
This deuterated compound does not have a biological target; it is a stable isotope-labeled chemical standard used in analytical chemistry for LC-MS quantification.
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| ln Vitro |
As a labeled internal standard, this compound is chemically identical in structure to the non-labeled linker and thus has identical biological properties as an ADC linker. Its "activity" is defined by its performance in bioanalytical methods, providing accurate, matrix-effect-corrected quantification.
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| ln Vivo |
This compound is not used in vivo for efficacy studies. It is used analytically to determine the concentration and biodistribution of the ADC linker in plasma and tissues from dosed animals.
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| Enzyme Assay |
General protocol for use as an internal standard: The linker (analyte) and FL118-C3-O-C-amide-C-NH2-d5 formate (internal standard) are added to a biological matrix. After protein precipitation with acetonitrile, the samples are analyzed by LC-MS/MS. Analyte concentrations are determined from the peak area ratio of analyte/IS, known as isotope dilution mass spectrometry.
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| Cell Assay |
This compound is not used in cell-based assays. It is used in the analysis of cellular fractions from ADC-treated cells to measure linker or catabolite concentrations. Cells are lysed, the internal standard is added, and the lysate is processed for LC-MS/MS analysis to quantify the amount of linker or payload released.
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| Animal Protocol |
The compound is not dosed directly to animals. It is used to analyze the PK of a related ADC linker. Plasma samples from ADC-dosed animals are spiked with this internal standard, and the linker or released payload concentrations are measured by LC-MS/MS to calculate PK parameters.
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| ADME/Pharmacokinetics |
Not applicable for the labeled standard. The PK properties of the non-labeled parent linker are assessed using this stable isotope as an internal standard in LC-MS/MS assays. Such assays provide accurate PK parameters like Cmax, Tmax, AUC, and half-life.
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| Toxicity/Toxicokinetics |
The toxicology of the non-labeled parent linker is assessed in animal models. The deuterated form is a highly controlled analytical reagent used only in validated LC-MS/MS methods, and it is not assessed for toxicity independently.
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| References | |
| Additional Infomation |
The molecular formula of this compound is C28H25D5N4O10, and its molecular weight is 587.59 g/mol. The formate salt form enhances its solubility in aqueous buffers. This product is intended for research use only and is not for human or veterinary diagnostic or therapeutic use.
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| Molecular Formula |
C28H25D5N4O10
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|---|---|
| Molecular Weight |
587.59
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| Appearance |
Solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 100 mg/mL (170.19 mM; with sonication)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7019 mL | 8.5093 mL | 17.0187 mL | |
| 5 mM | 0.3404 mL | 1.7019 mL | 3.4037 mL | |
| 10 mM | 0.1702 mL | 0.8509 mL | 1.7019 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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