| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Various methyltransferases. S-Adenosyl-L-methionine (SAMe) is the primary biological methyl donor in cells. It donates methyl groups to a wide range of substrates, including DNA, RNA, proteins, and lipids, in reactions catalyzed by methyltransferases. This methylation is crucial for regulating gene expression, protein function, and metabolism. It also serves as a precursor for the synthesis of polyamines and glutathione. Its broad role as a methyl donor makes it a key regulator of many cellular processes.
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| ln Vitro |
In vitro, S-Adenosyl-L-methionine chloride dihydrochloride is used as a cofactor in methylation assays. It is incubated with a methyltransferase enzyme and a substrate to measure enzyme activity. In cell culture, it has been shown to have anti-proliferative, pro-apoptotic, and anti-metastatic effects in various cancer cell lines. It can modulate cellular methylation status, affecting gene expression and signaling pathways. Its effects are concentration-dependent and cell-type specific. It is a standard reagent for studying methylation biology.
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| ln Vivo |
In vivo, S-Adenosyl-L-methionine chloride dihydrochloride has been studied for its antidepressant effects. It is available as a dietary supplement. In animal models of depression, SAMe has shown efficacy in improving behavioral outcomes. Its anti-proliferative and pro-apoptotic roles have been explored in cancer models, where it can inhibit tumor growth and metastasis. It is metabolized in the liver and participates in the methionine cycle. Its in vivo effects are attributed to its role as a methyl donor.
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| Enzyme Assay |
In vitro enzyme assays for S-Adenosyl-L-methionine involve measuring methyltransferase activity. The compound is used as a substrate in reactions with a methyltransferase enzyme, a substrate (e.g., DNA, histone), and a radiolabeled or fluorescent detection system. The transfer of the methyl group from SAMe to the substrate is quantified. These assays are performed in buffer solutions at physiological pH and temperature. The compound's purity and concentration are critical for accurate measurements. It is a standard reagent in these enzymatic assays.
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| Cell Assay |
In vitro cell-based assays using S-Adenosyl-L-methionine are performed in various cancer cell lines. Cells are treated with the compound, and effects on cell proliferation (MTT assay), apoptosis (Annexin V/PI staining), and metastasis (migration/invasion assays) are assessed. The global methylation status of DNA and histones can be measured using specific antibodies. Gene expression changes are analyzed by qPCR or RNA-seq. These assays are used to study the biological effects of methylation modulation.
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| Animal Protocol |
In vivo animal experiments with S-Adenosyl-L-methionine have been conducted in models of depression and cancer. In depression models, rodents are administered SAMe, and behavioral tests (e.g., forced swim test) are performed. In cancer models, tumor-bearing mice are treated with SAMe, and tumor growth and metastasis are monitored. Plasma and tissue levels of SAMe are measured. These studies demonstrate its in vivo efficacy and mechanism of action.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of S-Adenosyl-L-methionine chloride dihydrochloride have been studied. It is orally bioavailable, although the absolute bioavailability is low due to first-pass metabolism. It is rapidly metabolized in the liver. It has a short plasma half-life. The compound is distributed to various tissues, including the brain. It crosses the blood-brain barrier. Its metabolism involves transmethylation and transsulfuration pathways. The chloride dihydrochloride salt form enhances its stability and solubility.
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| Toxicity/Toxicokinetics |
Toxicological data for S-Adenosyl-L-methionine chloride dihydrochloride are available from its use as a dietary supplement. It is generally well-tolerated at recommended doses. Common side effects include gastrointestinal discomfort, nausea, and diarrhea. At high doses, it may cause mania in susceptible individuals. No significant organ toxicity has been reported. It is considered safe for use as a supplement. However, as a research chemical, standard precautions apply.
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| References | |
| Additional Infomation |
S-Adenosyl-L-methionine chloride dihydrochloride is a methyl donor and cofactor. It is also known as Ademetionine chloride dihydrochloride. Its molecular formula is C15H25Cl3N6O5S, and its molecular weight is 507.82. It is a dietary supplement with antidepressant effects and has anti-proliferative, pro-apoptotic, and anti-metastatic roles in cancer. It is a stabilized salt form used for research and supplementation.
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| Molecular Formula |
C15H25CL3N6O5S
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|---|---|
| Molecular Weight |
507.82
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| Exact Mass |
506.067
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| CAS # |
86867-01-8
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| PubChem CID |
71307500
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| Appearance |
White to light yellow solid powder
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| Hydrogen Bond Donor Count |
7
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| Hydrogen Bond Acceptor Count |
11
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
30
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| Complexity |
533
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| Defined Atom Stereocenter Count |
5
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| SMILES |
C[S+](CC[C@@H](C(=O)O)N)C[C@@H]1[C@H]([C@H]([C@@H](O1)N2C=NC3=C(N=CN=C32)N)O)O.Cl.Cl.[Cl-]
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| InChi Key |
KBAFOJZCBYWKPU-XQVUROGGSA-N
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| InChi Code |
InChI=1S/C15H22N6O5S.3ClH/c1-27(3-2-7(16)15(24)25)4-8-10(22)11(23)14(26-8)21-6-20-9-12(17)18-5-19-13(9)21;;;/h5-8,10-11,14,22-23H,2-4,16H2,1H3,(H2-,17,18,19,24,25);3*1H/t7-,8+,10+,11+,14+,27?;;;/m0.../s1
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| Chemical Name |
[(3S)-3-amino-3-carboxypropyl]-[[(2S,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl]-methylsulfanium;chloride;dihydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Typically soluble in DMSO (e.g. 10 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9692 mL | 9.8460 mL | 19.6920 mL | |
| 5 mM | 0.3938 mL | 1.9692 mL | 3.9384 mL | |
| 10 mM | 0.1969 mL | 0.9846 mL | 1.9692 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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