| Size | Price | Stock | Qty |
|---|---|---|---|
| 500g |
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| 1kg |
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| 2kg |
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| 5kg |
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| Targets |
Not applicable (cell culture reagent; no specific drug target). Magnesium sulfate provides Mg2+ ions essential for numerous enzymatic reactions, membrane stability, and cellular metabolism. Magnesium is a cofactor for hundreds of enzymes, including kinases, phosphatases, and ATPases. It is also essential for the structure and function of nucleic acids, ribosomes, and cell membranes.
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|---|---|
| ln Vitro |
Magnesium sulfate heptahydrate is a cell culture reagent with no direct biological activity beyond providing essential Mg2+ ions. Mg2+ ions serve as cofactors for ATP-dependent enzymes, stabilize cell membranes, and support cellular metabolism in vitro. In cell culture media, magnesium is an essential nutrient that must be provided for optimal cell growth and function. The concentration of magnesium in cell culture media is typically optimized for specific cell types and applications.
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| ln Vivo |
Not applicable (cell culture reagent; no in vivo activity). Magnesium sulfate reduces preterm labor in high-risk pregnant women and has laxative and constipation-relieving properties as a magnesium salt. In vivo, magnesium is an essential mineral that is involved in numerous physiological processes, including neuromuscular function, cardiovascular function, and bone metabolism. However, magnesium sulfate heptahydrate for cell culture is not intended for in vivo pharmacological studies.
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| Enzyme Assay |
Not applicable (cell culture reagent; no enzyme/receptor binding assays). Purity and composition are verified by chemical analysis (e.g., assay, heavy metals, loss on drying) per pharmacopoeial standards. The product is tested for suitability for cell culture applications, ensuring that it is free from contaminants that could affect cell growth.
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| Cell Assay |
Cell culture media (e.g., DMEM, RPMI, plant tissue culture media) are supplemented with magnesium sulfate heptahydrate at specific concentrations (typically 0.1-1.0 g/L). The medium is prepared by dissolving the appropriate amount of magnesium sulfate heptahydrate in water, along with other medium components, and sterilizing by filtration or autoclaving. Cell growth, morphology, and viability are monitored to confirm media quality and performance. The medium is used for the culture of various cell types, including mammalian cells, insect cells, and plant cells.
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| Animal Protocol |
Not applicable (cell culture reagent; not used for in vivo animal experiments). Magnesium sulfate is used clinically for the management of pre-eclampsia and preterm labor via intravenous administration. However, magnesium sulfate heptahydrate for cell culture is not intended for in vivo use.
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| ADME/Pharmacokinetics |
Magnesium sulfate heptahydrate is a cell culture reagent with no pharmacological pharmacokinetic properties. It is highly soluble in water and dissociates into Mg2+ and sulfate ions. In vivo, magnesium is an essential mineral with renal clearance. The compound is hygroscopic and should be stored in a tightly sealed container in a cool, dry place, protected from moisture and heat.
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| Toxicity/Toxicokinetics |
Magnesium sulfate heptahydrate is a cell culture-grade reagent with low toxicity at recommended concentrations. Standard laboratory safety practices apply. It may cause gastrointestinal effects (diarrhea) if ingested in large quantities. The compound is not intended for human consumption. Appropriate personal protective equipment (PPE) should be used when handling the powder to avoid inhalation and contact with skin and eyes.
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| References | |
| Additional Infomation |
Magnesium sulfate heptahydrate is the heptahydrate form of magnesium sulfate. It has laxative and constipation-relieving properties. It is a magnesium salt and also a hydrate. It contains magnesium sulfate. Epsom salt (magnesium sulfate) is a mineral with the chemical formula MgS6+O4·7H2O or Mg(SO4)·7H2O. The symbol of the International Mineralogical Association (IMA) is Esm.
Magnesium sulfate heptahydrate is a cell culture reagent and biochemical assay reagent. Molecular formula: MgSO4·7H2O, molecular weight: 246.47 g/mol. It is suitable for cell culture, molecular biology, and plant tissue culture applications. It has laxative and constipation-relieving properties. CAS: 10034-99-8. Synonyms: Magnesium sulfate heptahydrate, Epsom salts. For research use only, not for human therapeutic use. |
| Molecular Formula |
MGSO4.7H2O
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|---|---|
| Molecular Weight |
246.47
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| Exact Mass |
246.01
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| CAS # |
10034-99-8
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| Related CAS # |
7487-88-9 (Parent)
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| PubChem CID |
24843
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| Appearance |
White to off-white solid powder
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| Density |
2.66
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| Boiling Point |
330ºC at 760 mmHg
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| Melting Point |
1124°C
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| Vapour Pressure |
3.35E-05mmHg at 25°C
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| Hydrogen Bond Donor Count |
7
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| Hydrogen Bond Acceptor Count |
11
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
13
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| Complexity |
62.2
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O.O.O.O.O.O.O.[O-]S(=O)(=O)[O-].[Mg+2]
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| InChi Key |
WRUGWIBCXHJTDG-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/Mg.H2O4S.7H2O/c;1-5(2,3)4;;;;;;;/h;(H2,1,2,3,4);7*1H2/q+2;;;;;;;;/p-2
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| Chemical Name |
magnesium;sulfate;heptahydrate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~200 mg/mL (~811.46 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.0573 mL | 20.2864 mL | 40.5729 mL | |
| 5 mM | 0.8115 mL | 4.0573 mL | 8.1146 mL | |
| 10 mM | 0.4057 mL | 2.0286 mL | 4.0573 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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