| Size | Price | Stock | Qty |
|---|---|---|---|
| 100g |
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| Other Sizes |
| Targets |
Not applicable (microbiological growth medium; no drug target). Tryptone Soya Agar is a nutrient medium designed to support microbial growth, not to interact with a specific biological target. The medium provides a general nutritional environment for the cultivation of microorganisms.
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|---|---|
| ln Vitro |
Tryptone Soya Agar supports the growth of a wide variety of microorganisms, including fastidious organisms such as Neisseria, Listeria, and Brucella. Tryptone and soya peptone provide nitrogenous and other nutrients required for microbial replication. The medium is used as a reference medium when testing selective media to measure the degree of inhibition. In quality control testing, the growth promotion properties of the medium are assessed by inoculating with standard reference strains and evaluating colony formation and morphology.
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| ln Vivo |
Not applicable (growth medium; no in vivo activity). Tryptone Soya Agar is used as a reference medium for testing selective media and measuring the degree of inhibition. It is not administered in vivo and has no pharmacological activity.
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| Enzyme Assay |
Quality control testing of Tryptone Soya Agar involves inoculating the medium with standard reference strains (e.g., Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans, Aspergillus brasiliensis) and incubating at appropriate temperatures (e.g., 30-35°C for bacteria, 20-25°C for fungi). Growth promotion (the ability of the medium to support growth of the test organisms) is assessed by comparing the number of colonies obtained on the test medium with that on a previously approved batch of medium. Colony morphology and characteristics are also evaluated. The medium must demonstrate adequate growth promotion to be considered suitable for use in pharmacopoeial testing.
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| Cell Assay |
Tryptone Soya Agar is not used for cell culture; it is a solid microbiological medium for bacterial and fungal cultivation. Inoculated plates are incubated under aerobic or anaerobic conditions depending on the target organism. The medium is used for the isolation and cultivation of nonfastidious and fastidious microorganisms. In pharmaceutical microbiology, it is used for environmental monitoring (settle plates, contact plates, air sampling), sterility testing, and microbial limit tests.
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| Animal Protocol |
Tryptone Soya Agar is not used for in vivo animal experiments; it is an in vitro microbiological growth medium. It is used for environmental monitoring, sterility testing, and microbial limit tests in pharmaceutical and food industries. The medium is also used for the isolation of Bacteroides gracilis by adding formate, fumarate, and nitrate.
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| ADME/Pharmacokinetics |
Tryptone Soya Agar is a dehydrated culture medium; not a drug with pharmacokinetic properties. It is reconstituted with purified water according to the manufacturer's instructions and sterilized by autoclaving (typically 121°C for 15 minutes) before use. Dehydrated medium is stored in a cool, dry place, protected from light and moisture. The prepared medium should be stored at 2-8°C and used within a specified period to maintain its growth-promoting properties.
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| Toxicity/Toxicokinetics |
Tryptone Soya Agar is a microbiological growth medium with no intrinsic toxicity. Standard laboratory safety practices apply when handling inoculated media (potential biohazard). The dehydrated powder may be irritant; appropriate personal protective equipment (PPE) is recommended when handling the powder to avoid inhalation of dust and contact with skin and eyes. The medium is not intended for human consumption.
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| References | |
| Additional Infomation |
Tryptone Soya Agar is a general-purpose microbiological medium widely used in pharmaceutical, food, and environmental microbiology for sterility testing, microbial limit tests, and environmental monitoring. It is specified in EP, USP, JP, and BP pharmacopoeias. Synonyms: Casein Soya Bean Digest Agar, TSA. The medium supports the growth of a wide variety of organisms, including fastidious ones such as Neisseria. It is recommended as a reference medium when testing selective media to measure the degree of inhibition. For research and quality control use only, not for human therapeutic use.
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| Appearance |
Light yellow to yellow solid powder
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|---|---|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Typically soluble in DMSO (e.g. 10 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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