| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Not applicable (microbiological growth medium; no drug target). TSB is a nutrient medium designed to support microbial growth, not to interact with a specific biological target. The medium provides a general nutritional environment for the cultivation of a wide range of microorganisms.
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|---|---|
| ln Vitro |
TSB supports the growth of a wide range of microorganisms. The combination of tryptone and soya peptone provides amino acids and long chain peptides for microbial growth. Dextrose is included as a carbon source. TSB is used for the cultivation of both aerobic and anaerobic microorganisms.
|
| ln Vivo |
Not applicable (growth medium; no in vivo activity). TSB is used as a microbiological growth medium for in vitro cultivation of microorganisms. It is not administered in vivo and has no pharmacological activity.
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| Enzyme Assay |
Quality control testing of TSB involves inoculating the medium with standard reference strains (e.g., Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans, Aspergillus brasiliensis) and incubating at appropriate temperatures (e.g., 30-35°C for bacteria, 20-25°C for fungi). Growth promotion is assessed by turbidity measurement or colony counting.
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| Cell Assay |
TSB is not used for mammalian cell culture; it is a liquid microbiological medium for bacterial and fungal cultivation. Inoculated broths are incubated under aerobic or anaerobic conditions depending on the target organism. Growth is assessed by turbidity, optical density measurement, or viable plate counts.
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| Animal Protocol |
TSB is not used for in vivo animal experiments; it is an in vitro microbiological growth medium. It is used for environmental monitoring, sterility testing, and microbial limit tests in pharmaceutical and food industries.
|
| ADME/Pharmacokinetics |
TSB is a dehydrated culture medium; not a drug with pharmacokinetic properties. It is reconstituted with purified water and sterilized by autoclaving before use. Dehydrated medium is stored in a cool, dry place. The prepared broth should be stored at 2-8°C and used within a specified period.
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| Toxicity/Toxicokinetics |
TSB is a microbiological growth medium with no intrinsic toxicity. Standard laboratory safety practices apply when handling inoculated media (potential biohazard). The dehydrated powder may be irritant; appropriate PPE is recommended. It is not intended for human consumption.
|
| References | |
| Additional Infomation |
TSB is a general-purpose microbiological medium widely used in pharmaceutical, food, and environmental microbiology. Synonyms: Soybean-Casein Digest Medium, Tryptic Soya Broth【25†15】. It conforms to USP, EP, and JP pharmacopoeias. It is used for sterility testing and microbial limit testing. For research and quality control use only.
|
| Appearance |
Typically exists as solid at room temperature
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|---|---|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
Typically soluble in DMSO (e.g. 10 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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