| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Not applicable (analytical standard; parent compound leucovorin targets dihydrofolate reductase). Leucovorin is a reduced form of folic acid that bypasses dihydrofolate reductase (DHFR) and serves as a source of tetrahydrofolate, a cofactor for nucleotide synthesis. It is used to rescue cells from the toxic effects of methotrexate, a DHFR inhibitor. Leucovorin-d4 is an analytical standard with no direct biological activity.
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|---|---|
| ln Vitro |
Leucovorin-d4 is a deuterated isotopologue of folinic acid (leucovorin), a biological folic acid. Leucovorin is used as a rescue agent to decrease methotrexate-induced toxicity. As a stable isotope-labeled compound, Leucovorin-d4 has no intrinsic biological activity beyond that of the parent compound. It is used as an internal standard for analytical quantification.
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| ln Vivo |
Leucovorin-d4 is not intended for in vivo pharmacological studies; it is used as an analytical standard. The parent compound leucovorin is used clinically as a rescue agent in methotrexate therapy. In vivo, leucovorin is converted to active tetrahydrofolate cofactors that support nucleotide synthesis and cell proliferation.
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| Enzyme Assay |
Not applicable for biological assays. Leucovorin-d4 is used as an internal standard for the quantification of leucovorin in biological samples by LC-MS/MS. It is spiked into samples at a known concentration prior to extraction and analysis. The ratio of the peak area of unlabeled leucovorin to that of Leucovorin-d4 is used to calculate the concentration of leucovorin in the sample.
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| Cell Assay |
Leucovorin-d4 is not used in cell-based assays; it is an analytical standard. It is used in analytical chemistry laboratories for the quantification of leucovorin in biological samples such as plasma, urine, and tissues. Quality control testing includes verification of isotopic purity and chemical purity.
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| Animal Protocol |
Leucovorin-d4 is not used in animal experiments except as an analytical standard for measuring leucovorin concentrations in pharmacokinetic studies. It is used as an internal standard in LC-MS/MS assays to quantify leucovorin levels in animal and human samples.
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| ADME/Pharmacokinetics |
Leucovorin-d4 is an analytical standard with no pharmacological pharmacokinetic properties. It is stable under recommended storage conditions. It is typically stored as a powder at -20°C. In analytical workflows, it is dissolved in suitable solvents at known concentrations for spiking into samples.
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| Toxicity/Toxicokinetics |
Leucovorin-d4 is a stable isotope-labeled compound with no intended biological use. Standard laboratory safety practices apply; it is not intended for human consumption. The parent compound leucovorin is a clinically approved drug with a well-established safety profile. Leucovorin-d4 itself is an analytical standard and is handled with standard chemical safety precautions.
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| References | |
| Additional Infomation |
Leucovorin-d4 (calcium hydrate), >90% is a deuterated isotopologue of folinic acid (leucovorin), used as an analytical internal standard. Leucovorin is a biological folic acid administered with methotrexate as a rescue agent. CAS: not provided. For research use only, not for human therapeutic use.
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| Molecular Formula |
C20H19D4CAN7O8
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|---|---|
| Molecular Weight |
533.54
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| Appearance |
Typically exists as solid at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Typically soluble in DMSO (e.g. 10 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8743 mL | 9.3714 mL | 18.7427 mL | |
| 5 mM | 0.3749 mL | 1.8743 mL | 3.7485 mL | |
| 10 mM | 0.1874 mL | 0.9371 mL | 1.8743 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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