| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| Other Sizes |
Purity: ≥98%
| Targets |
Not applicable (chemical crosslinker; no specific drug target). Azide-A-DSBSO crosslinker is a chemical tool used for studying protein-protein interactions and protein complex structures. It does not target a specific biological receptor or enzyme; rather, it covalently crosslinks proteins that are in close proximity, enabling structural and interaction studies by mass spectrometry.
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| ln Vitro |
Azide-A-DSBSO crosslinker is a chemical crosslinker with no direct biological activity. It reacts with lysine residues on proteins via NHS ester groups, forming covalent crosslinks between interacting proteins. The azide group enables click chemistry for further functionalization. The acid-cleavable and MS-cleavable features facilitate identification of crosslinked peptides by mass spectrometry.
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| ln Vivo |
Azide-A-DSBSO crosslinker is not intended for in vivo pharmacological studies; it is used as a chemical tool for structural biology and proteomics. It has potential applications in drug discovery, proteomics, and targeted therapeutics. It is used to study protein dynamics, interactions, and complex formation in cells and tissues.
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| Enzyme Assay |
Not applicable for biological assays. Azide-A-DSBSO crosslinker is used in chemical crosslinking mass spectrometry (XL-MS) workflows. Proteins or protein complexes are incubated with the crosslinker, and crosslinked products are analyzed by mass spectrometry. The MS-cleavable feature allows identification of crosslinked peptides and mapping of protein interaction interfaces.
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| Cell Assay |
Azide-A-DSBSO crosslinker is membrane-permeable and can be used in cell-based crosslinking experiments. Cells are treated with the crosslinker, which enters cells and crosslinks intracellular proteins via NHS ester-lysine reactions. Crosslinked proteins are then extracted, digested with proteases, and analyzed by mass spectrometry to identify protein-protein interactions and protein complexes.
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| Animal Protocol |
Azide-A-DSBSO crosslinker is not typically used in animal experiments. It is used as a chemical tool in structural biology and proteomics research. If used in vivo, it would be administered to tissues or organisms, but this is not standard practice due to the complexity of crosslinking in whole organisms.
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| ADME/Pharmacokinetics |
Azide-A-DSBSO crosslinker is a chemical reagent with no pharmacological pharmacokinetic properties. It is soluble in appropriate organic solvents and can be used in aqueous buffers for crosslinking reactions. It is typically stored as a powder at -20°C. The compound is stable under recommended storage conditions.
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| Toxicity/Toxicokinetics |
Azide-A-DSBSO crosslinker is a chemical reagent handled with standard laboratory safety practices. It is not intended for in vivo administration. Appropriate personal protective equipment (PPE) should be used when handling the compound. Toxicity data are not available; standard chemical safety precautions apply.
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| References | |
| Additional Infomation |
Azide-A-DSBSO crosslinker is a research-grade chemical crosslinker for studying protein-protein interactions by crosslinking mass spectrometry (XL-MS). CAS: 1704097-02-8. It is a mass spectrometry-cleavable, membrane-permeable, homobifunctional, azide-labeled, acid-cleavable crosslinking peptide. It crosslinks proteins via NHS ester reaction with lysine residues. For research use only.
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| Molecular Formula |
C24H33N5O12S2
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|---|---|
| Molecular Weight |
647.675123929977
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| Exact Mass |
647.156
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| CAS # |
1704097-02-8
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| PubChem CID |
122221677
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| Appearance |
White to off-white solid powder
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| LogP |
-2.3
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
16
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| Rotatable Bond Count |
18
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| Heavy Atom Count |
43
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| Complexity |
1130
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC1(OCC(CO1)(CS(=O)CCC(=O)ON2C(=O)CCC2=O)CS(=O)CCC(=O)ON3C(=O)CCC3=O)CCCN=[N+]=[N-]
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| InChi Key |
AKLJWXIVLPURNY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C24H33N5O12S2/c1-23(9-2-10-26-27-25)38-13-24(14-39-23,15-42(36)11-7-21(34)40-28-17(30)3-4-18(28)31)16-43(37)12-8-22(35)41-29-19(32)5-6-20(29)33/h2-16H2,1H3
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| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) 3-[[2-(3-azidopropyl)-5-[[3-(2,5-dioxopyrrolidin-1-yl)oxy-3-oxopropyl]sulfinylmethyl]-2-methyl-1,3-dioxan-5-yl]methylsulfinyl]propanoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO :~100 mg/mL (~154.40 mM; with sonication)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5440 mL | 7.7199 mL | 15.4397 mL | |
| 5 mM | 0.3088 mL | 1.5440 mL | 3.0879 mL | |
| 10 mM | 0.1544 mL | 0.7720 mL | 1.5440 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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