| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| Other Sizes |
| Targets |
Cyanine3 amine (TFA) is a fluorescent labeling reagent and does not have a specific pharmacological target. It is designed to label biomolecules such as proteins, peptides, and nucleic acids through covalent conjugation via its primary amine group. The dye is used for fluorescence microscopy, flow cytometry, and other fluorescence-based detection applications.
|
|---|---|
| ln Vitro |
As a fluorescent probe, Cyanine3 amine (TFA) itself does not possess pharmacological activity. Its utility lies in its photophysical properties: it is a bright, photostable green-fluorescent dye with excitation at 555 nm and emission at 570 nm. The dye exhibits good quantum yield and is suitable for a wide range of fluorescence-based biological applications.
|
| ln Vivo |
As a fluorescent probe, Cyanine3 amine (TFA) is used for in vivo imaging applications. When conjugated to targeting moieties, the dye enables visualization of specific biomarkers in living organisms. The green fluorescence (ex/em ~555/570 nm) allows for multiplexing with red and far-red dyes, but tissue penetration is limited compared to near-infrared dyes.
|
| Enzyme Assay |
Non-cell-based protocols for Cyanine3 amine (TFA) involve conjugation chemistry and dye characterization. A typical conjugation procedure includes: dissolving the dye in anhydrous DMSO, adding to a solution of the target molecule containing NHS ester or carboxy groups in appropriate buffer (pH 7.5–8.5), incubating at room temperature for 1–4 hours, and purifying the conjugate by size-exclusion chromatography or dialysis. Labeling efficiency is determined by spectrophotometric measurement.
|
| Cell Assay |
Cellular assays with Cyanine3 amine (TFA) conjugates typically involve labeling cells for fluorescence imaging or flow cytometry. A representative protocol includes: culturing cells, incubating with the Cyanine3-labeled probe at 0.1–10 μM for 30–120 minutes at 37°C, washing with PBS, fixing cells if necessary, and imaging using fluorescence microscopy with standard Cy3 filter sets (excitation 540–560 nm, emission 570–590 nm) or analyzing by flow cytometry.
|
| Animal Protocol |
In vivo animal imaging studies using Cyanine3 amine conjugates are less common due to the visible-range fluorescence (limited tissue penetration). When used, a typical protocol includes: intravenous or intraperitoneal administration of the dye-conjugated probe into mouse models, allowing distribution for 1–24 hours, and performing fluorescence imaging using appropriate excitation/emission filters.
|
| ADME/Pharmacokinetics |
Tissue autofluorescence and light scattering limit the use of Cyanine3 for deep-tissue imaging.
|
| Toxicity/Toxicokinetics |
Pharmacokinetic properties are determined for the Cyanine3 amine conjugate rather than the dye itself. Typical PK parameters for dye-conjugated small molecules or peptides include rapid clearance, distribution depending on the targeting moiety, and excretion primarily via renal or hepatobiliary routes. The dye itself is not intended as a therapeutic agent.
|
| References | |
| Additional Infomation |
As a fluorescent dye for research use, Cyanine3 amine (TFA) is not intended for human therapeutic use. Standard safety precautions should be followed when handling the dye. The compound should be stored at 4°C in a sealed container, protected from moisture and light. Stock solutions in DMSO can be stored at -80°C for 6 months or -20°C for 1 month.
|
| Molecular Formula |
C38H51F3N4O3
|
|---|---|
| Molecular Weight |
668.83
|
| Appearance |
Light brown to brown solid powder
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
Typically soluble in DMSO (e.g. 10 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4951 mL | 7.4757 mL | 14.9515 mL | |
| 5 mM | 0.2990 mL | 1.4951 mL | 2.9903 mL | |
| 10 mM | 0.1495 mL | 0.7476 mL | 1.4951 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
