| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
As a deuterated internal standard, N1-(4-Nitrophenyl)sulfanilamide-d4 does not have a specific pharmacological target. The unlabeled parent compound, N1-(4-Nitrophenyl)sulfanilamide (sulfanitran), is a sulfonamide antimicrobial agent that targets bacterial dihydropteroate synthase (DHPS), an enzyme involved in the folate synthesis pathway. Sulfonamides inhibit this enzyme by competing with para-aminobenzoic acid (PABA).
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| ln Vitro |
As an isotope-labeled internal standard, N1-(4-Nitrophenyl)sulfanilamide-d4 itself does not exhibit antibacterial activity. The unlabeled parent compound sulfanitran is a sulfonamide antibiotic with in vitro activity against various bacterial pathogens and coccidial parasites. It acts as a competitive inhibitor of dihydropteroate synthase, blocking folate synthesis and thereby inhibiting nucleic acid production.
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| ln Vivo |
As a deuterated internal standard, N1-(4-Nitrophenyl)sulfanilamide-d4 itself does not exhibit in vivo activity. The unlabeled parent compound sulfanitran is used as an anticoccidial drug in poultry, added to drinking water to treat coccidiosis caused by Eimeria species. It is effective against various stages of the parasite's life cycle in the intestinal tract.
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| Enzyme Assay |
Non-cell-based assay protocols for N1-(4-Nitrophenyl)sulfanilamide-d4 involve LC-MS/MS analysis. A typical workflow includes: sample preparation (extraction with organic solvents, protein precipitation, or SPE), chromatographic separation on a reversed-phase C18 column with a mobile phase of water/acetonitrile containing 0.1% formic acid, and MS/MS detection in MRM mode using the characteristic mass transitions of the deuterated compound.
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| Cell Assay |
Cell-based assays are not applicable to N1-(4-Nitrophenyl)sulfanilamide-d4 as it is an analytical standard. The unlabeled parent compound sulfanitran may be evaluated in cell-based antimicrobial susceptibility testing against bacterial or protozoal pathogens to determine its minimum inhibitory concentration (MIC) using standard broth microdilution methods.
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| Animal Protocol |
In vivo animal studies with N1-(4-Nitrophenyl)sulfanilamide-d4 are not conducted as it is an analytical standard. The unlabeled parent compound sulfanitran is administered to poultry via drinking water for the treatment of coccidiosis. Drug efficacy is evaluated by monitoring clinical signs, oocyst shedding, and weight gain in infected birds.
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| ADME/Pharmacokinetics |
As an isotope-labeled internal standard, N1-(4-Nitrophenyl)sulfanilamide-d4 has no pharmacokinetic properties of its own. The unlabeled parent compound sulfanitran, when administered to poultry, is absorbed from the gastrointestinal tract and distributed to various tissues, with elimination primarily via renal excretion.
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| Toxicity/Toxicokinetics |
As a research-use isotope-labeled compound, N1-(4-Nitrophenyl)sulfanilamide-d4 is not intended for human therapeutic use. Toxicological data are not applicable to this analytical standard. The unlabeled parent compound sulfanitran has a well-established safety profile in poultry at recommended therapeutic doses.
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| Additional Infomation |
N1-(4-Nitrophenyl)sulfanilamide-d4 (4-Amino-N-(4-nitrophenyl)benzenesulfonamide-d4) is a stable isotope-labeled internal standard for analytical chemistry applications. It is typically stored at -20°C for long-term preservation. The deuterium labeling provides a mass shift that enables accurate quantification of sulfonamide residues in food safety and environmental monitoring applications.
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| Molecular Formula |
C12H7D4N3O4S
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| Molecular Weight |
297.32
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| Appearance |
Off-white to yellow solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Typically soluble in DMSO (e.g. 10 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3634 mL | 16.8169 mL | 33.6338 mL | |
| 5 mM | 0.6727 mL | 3.3634 mL | 6.7268 mL | |
| 10 mM | 0.3363 mL | 1.6817 mL | 3.3634 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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