| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
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| Other Sizes |
| Targets |
As a stable isotope-labeled internal standard, Squalene-d6 does not have a pharmacological target. The unlabeled parent compound squalene is an intermediate product in cholesterol biosynthesis and has various pharmacological properties including lipid-lowering, hepatoprotective, anti-atherosclerotic, cardioprotective, antioxidant, and antitumor activities.
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|---|---|
| ln Vitro |
As an isotope-labeled analytical standard, Squalene-d6 itself does not exhibit biological activity in vitro. The unlabeled parent compound squalene has been studied for various bioactivities including antioxidant properties and immunomodulatory effects. In cell-free systems, squalene demonstrates free radical scavenging activity and acts as a quencher of singlet oxygen.
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| ln Vivo |
As an isotope-labeled analytical standard, Squalene-d6 itself does not exhibit biological activity in vivo. The unlabeled parent compound squalene is a natural triterpene found in high concentrations in shark liver oil and olive oil, and is used as an immunologic adjuvant in vaccine formulations (e.g., AddaVax, MF59). It enhances immune responses through stimulation of antigen-presenting cells.
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| Enzyme Assay |
Non-cell-based assay protocols for Squalene-d6 typically involve LC-MS/MS or GC-MS analysis. A representative workflow includes: sample extraction with organic solvents (e.g., hexane or chloroform:methanol), saponification if necessary, solid-phase extraction cleanup, chromatographic separation on a C18 or DB-5 column, and MS detection in selected ion monitoring (SIM) or MRM mode. The deuterated compound serves as an internal standard for quantification of endogenous squalene.
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| Cell Assay |
Cell-based assays are not applicable to Squalene-d6 as it is an isotope-labeled analytical standard. However, the unlabeled parent compound squalene may be evaluated in cell culture models for its effects on cell viability, lipid metabolism, and oxidative stress using standard cytotoxicity assays (MTT, LDH release) and biochemical marker measurements.
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| Animal Protocol |
In vivo studies are not conducted with Squalene-d6 as it is an analytical standard rather than a therapeutic agent. The unlabeled parent compound squalene has been studied in animal models for its immunoadjuvant properties, typically administered as an emulsion component in vaccine formulations via intramuscular or subcutaneous injection.
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| ADME/Pharmacokinetics |
Dosing and pharmacokinetic studies are not applicable to Squalene-d6 as it is an analytical standard. The unlabeled parent compound squalene, when administered in vaccine adjuvants, exhibits prolonged retention at the injection site with slow absorption into systemic circulation, and is primarily metabolized through the cholesterol biosynthesis pathway.
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| Toxicity/Toxicokinetics |
Squalene-d6 is a research-use stable isotope-labeled compound and is not intended for human therapeutic use. Toxicological data are not applicable to this analytical standard. The unlabeled parent compound squalene is generally recognized as safe (GRAS) when used in food and cosmetic products, and is approved as an adjuvant component in certain human vaccines (e.g., MF59-adjuvanted influenza vaccines).
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| References | |
| Additional Infomation |
Squalene-d6 (CAS 1875101-86-2) is a valuable tool for quantitative bioanalysis, metabolic tracing, and stable isotope dilution assays. The deuterium labeling has been shown to enhance the oxidative stability of squalene compared to its unlabeled counterpart. This compound is also referred to as Super Squalene-d6, trans-Squalene-d6, and AddaVax-d6. It is typically stored at -20°C, protected from light, and under nitrogen atmosphere.
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| Molecular Formula |
C30H50
|
|---|---|
| Molecular Weight |
416.754979610443
|
| Exact Mass |
416.428
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| CAS # |
1875101-86-2
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| PubChem CID |
71752234
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| Appearance |
Colorless to light yellow liquid
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| LogP |
11.6
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
0
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| Rotatable Bond Count |
15
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| Heavy Atom Count |
30
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| Complexity |
578
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
C(/C(/C)=C/CC/C=C(\C)/CC/C=C(\C)/CC/C=C(\C)/C)C/C=C(\C)/CC/C=C(\C([2H])([2H])[2H])/C([2H])([2H])[2H]
|
| InChi Key |
YYGNTYWPHWGJRM-FSAUMQCISA-N
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| InChi Code |
InChI=1S/C30H50/c1-25(2)15-11-19-29(7)23-13-21-27(5)17-9-10-18-28(6)22-14-24-30(8)20-12-16-26(3)4/h15-18,23-24H,9-14,19-22H2,1-8H3/b27-17+,28-18+,29-23+,30-24+/i1D3,2D3
|
| Chemical Name |
(6E,10E,14E,18E)-1,1,1-trideuterio-6,10,15,19,23-pentamethyl-2-(trideuteriomethyl)tetracosa-2,6,10,14,18,22-hexaene
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Typically soluble in DMSO (e.g. 10 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3995 mL | 11.9973 mL | 23.9946 mL | |
| 5 mM | 0.4799 mL | 2.3995 mL | 4.7989 mL | |
| 10 mM | 0.2399 mL | 1.1997 mL | 2.3995 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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