| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Targets |
Ac-YEVD-AMC targets the enzymatic activities of caspase-1 and caspase-11 (also known as inflammatory caspases). Caspase-1 is a key enzyme in the inflammasome pathway that cleaves pro-IL-1β and pro-IL-18 to their active forms, playing a central role in inflammatory responses. Caspase-11 is a murine caspase that responds to intracellular LPS and also activates the inflammasome. Ac-YEVD-AMC serves as a substrate for these enzymes, enabling the measurement of their catalytic activity through the release of fluorescent AMC.
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| ln Vitro |
As a substrate rather than an inhibitor, Ac-YEVD-AMC itself does not possess inhibitory activity against caspases. Its utility lies in its ability to be cleaved by caspase-1 and caspase-11, releasing the fluorescent AMC moiety. The compound is used in enzymatic assays to measure caspase-1 and caspase-11 activity, with the rate of fluorescence increase being proportional to enzyme activity. Ac-YEVD-AMC is not evaluated for traditional in vitro activity such as IC50 values against cell lines.
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| ln Vivo |
Ac-YEVD-AMC is not a therapeutic compound and is not evaluated for in vivo activity in the traditional sense. Its primary application is in biochemical assays for measuring caspase-1 and caspase-11 activity in vitro and in cell lysates. The compound could potentially be used in ex vivo assays to measure inflammasome activation in tissue lysates or in imaging applications to visualize caspase activity.
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| Enzyme Assay |
Non-cell-based enzyme assays with Ac-YEVD-AMC typically involve incubating the substrate with purified caspase-1 or caspase-11 enzyme in an appropriate buffer system. A standard protocol includes: preparing a reaction buffer (pH 7.4, containing 100 mM HEPES, 10% sucrose, 0.1% CHAPS, and 10 mM DTT), adding Ac-YEVD-AMC at concentrations ranging from 1 to 100 μM, initiating the reaction by adding the enzyme, and incubating at 37°C for 30–60 minutes. The reaction is stopped by adding a stop solution (e.g., 10 mM EDTA), and fluorescence is measured at excitation/emission wavelengths of 380/460 nm for AMC.
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| Cell Assay |
Cellular assays with Ac-YEVD-AMC are typically performed using cell lysates from inflammasome-activated cells or live cells. A representative protocol for cell lysate assays includes: lysing cells in a suitable buffer (e.g., 50 mM HEPES, 0.1% CHAPS, 5 mM EDTA, 5 mM DTT), incubating the lysate with Ac-YEVD-AMC at 37°C for 1–2 hours, and measuring fluorescence at 380/460 nm. For live-cell assays, cells are incubated with a cell-permeable version of the substrate, and fluorescence is monitored using a fluorescence plate reader or microscope.
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| Animal Protocol |
In vivo animal studies with Ac-YEVD-AMC are not typically conducted as the compound is a research substrate rather than a therapeutic agent. However, the compound could potentially be used in animal models to assess caspase-1 or caspase-11 activity in tissues following administration. The compound is not intended for therapeutic development and is used exclusively as a research tool.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not typically evaluated for Ac-YEVD-AMC as it is a research substrate rather than a therapeutic compound. The compound is soluble in DMSO at 100 mg/mL (138.17 mM) and is typically stored at -20°C in a sealed container, protected from moisture and light. For use in assays, the compound is dissolved in DMSO and diluted in aqueous buffer.
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| Toxicity/Toxicokinetics |
As a research substrate, Ac-YEVD-AMC is not intended for human therapeutic use, and comprehensive toxicological data are not available. Standard laboratory safety precautions should be followed when handling the compound. The compound is typically stored at -20°C in a sealed container, protected from moisture and light.
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| References | |
| Additional Infomation |
Ac-YEVD-AMC (CAS 1631716-11-4) is a fluorogenic substrate for caspase-1 and caspase-11, widely used in inflammasome research. Upon enzymatic cleavage, the release of AMC enables sensitive and quantitative detection of caspase activity using fluorescence spectroscopy. This substrate is used in high-throughput screening campaigns to identify inhibitors of caspase-1 and caspase-11, as well as in basic research to study inflammasome activation in various biological contexts. Ac-YEVD-AMC is a valuable tool for studying inflammatory diseases, including autoinflammatory disorders, sepsis, and neurodegenerative diseases.
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| Molecular Formula |
C35H41N5O12
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| Molecular Weight |
723.726349592209
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| Exact Mass |
723.275
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| CAS # |
1631716-11-4
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| PubChem CID |
121235614
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
8
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| Hydrogen Bond Acceptor Count |
12
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| Rotatable Bond Count |
17
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| Heavy Atom Count |
52
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| Complexity |
1390
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| Defined Atom Stereocenter Count |
4
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| SMILES |
O=C([C@H](CCC(=O)O)NC([C@H](CC1C=CC(=CC=1)O)NC(C)=O)=O)N[C@H](C(N[C@H](C(NC1=CC=C2C(C)=CC(=O)OC2=C1)=O)CC(=O)O)=O)C(C)C
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| InChi Key |
GQCNHDNGOLXFDI-VIYORZMQSA-N
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| InChi Code |
InChI=1S/C35H41N5O12/c1-17(2)31(35(51)39-26(16-29(45)46)33(49)37-21-7-10-23-18(3)13-30(47)52-27(23)15-21)40-32(48)24(11-12-28(43)44)38-34(50)25(36-19(4)41)14-20-5-8-22(42)9-6-20/h5-10,13,15,17,24-26,31,42H,11-12,14,16H2,1-4H3,(H,36,41)(H,37,49)(H,38,50)(H,39,51)(H,40,48)(H,43,44)(H,45,46)/t24-,25-,26-,31-/m0/s1
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| Chemical Name |
(4S)-4-[[(2S)-2-acetamido-3-(4-hydroxyphenyl)propanoyl]amino]-5-[[(2S)-1-[[(2S)-3-carboxy-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxopropan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-5-oxopentanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO :~100 mg/mL (~138.17 mM; with sonication)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3817 mL | 6.9087 mL | 13.8173 mL | |
| 5 mM | 0.2763 mL | 1.3817 mL | 2.7635 mL | |
| 10 mM | 0.1382 mL | 0.6909 mL | 1.3817 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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