| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
6-HEX is a fluorescent labeling reagent and does not have a specific pharmacological target. It is designed to label nucleic acid sequences through covalent attachment, enabling detection and quantification in various fluorescence-based assays. The dye's fluorescence properties (excitation at 532 nm, emission at 556 nm) make it suitable for use with argon laser excitation at 543 nm.
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| ln Vitro |
When using 6-HEX, further dilution with deionized water is possible, resulting in fluorophore concentrations ranging from 300 nM to 20 µM.
As a fluorescent probe, 6-HEX itself does not possess pharmacological activity. Its utility lies in its photophysical properties: it is a bright, photostable fluorescent dye with excitation at 532 nm and emission at 556 nm. The dye is used in nucleic acid labeling, real-time PCR, and fluorescence in situ hybridization (FISH) applications. |
| ln Vivo |
6-HEX is not a therapeutic compound and is not evaluated for in vivo activity in the traditional sense. Its primary application is in biochemical and molecular biology assays for nucleic acid detection and quantification. The dye can be used to label nucleic acid sequences for detection in complex biological samples.
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| Enzyme Assay |
Non-cell-based protocols for 6-HEX typically involve labeling nucleic acid sequences with the dye. A standard protocol includes dissolving 6-HEX in anhydrous DMSO, activating the dye if necessary (e.g., as an NHS ester for amine coupling), incubating with an amine-modified oligonucleotide in carbonate/bicarbonate buffer (pH 8.5–9.0) at room temperature for 1–4 hours, and purifying the labeled oligonucleotide by ethanol precipitation or HPLC. Labeling efficiency is determined by measuring absorbance at 532 nm.
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| Cell Assay |
Cellular assays with 6-HEX typically involve fluorescence in situ hybridization (FISH) or real-time PCR applications. A representative protocol for FISH includes fixing cells on slides, hybridizing with 6-HEX-labeled probes at appropriate temperatures, washing to remove unbound probes, and imaging using fluorescence microscopy with appropriate excitation/emission filters (excitation 532 nm, emission 556 nm). The dye's fluorescence enables detection of specific nucleic acid sequences in cells or tissues.
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| Animal Protocol |
In vivo animal studies with 6-HEX are not typically conducted as the compound is a fluorescent dye used in molecular biology applications rather than a therapeutic agent. The dye is used in ex vivo analysis of tissues and cells.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not typically evaluated for 6-HEX as it is a research dye rather than a therapeutic compound. The compound is soluble in DMSO at 125 mg/mL. 6-HEX is typically stored at -20°C, protected from light.
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| Toxicity/Toxicokinetics |
As a fluorescent dye for research use, 6-HEX is not intended for human therapeutic use, and comprehensive toxicological data are not available. Standard laboratory safety precautions should be followed when handling the dye. The compound is stored at -20°C, protected from light.
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| References | |
| Additional Infomation |
6-HEX (CAS 155911-16-3) is a fluorescent dye used for labeling nucleic acid sequences and designing optical materials. The fluorophore has excitation and emission wavelengths of 532 nm and 556 nm, respectively. 6-HEX-labeled nucleic acids can be identified using argon laser radiation at 543 nm, and emission spectra at 550 nm and 650 nm can be used to locate genes in complex tissues or heterogeneous cell populations. The dye is widely used in real-time quantitative PCR (qRT-PCR) assays.
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| Molecular Formula |
C21H6CL6O7
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|---|---|
| Molecular Weight |
582.99
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| Exact Mass |
579.824
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| CAS # |
155911-16-3
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| PubChem CID |
10188533
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| Appearance |
Off-white to pink solid powder
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| Density |
2.06±0.1 g/cm3(Predicted)
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| Boiling Point |
794.8±60.0 °C(Predicted)
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| LogP |
7.284
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
34
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| Complexity |
830
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| Defined Atom Stereocenter Count |
0
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| SMILES |
OC(=O)C1=CC(Cl)=C2C(=O)OC3(C2=C1Cl)C1=CC(Cl)=C(O)C(Cl)=C1OC1=C(Cl)C(O)=C(Cl)C=C31
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| InChi Key |
AFBUYXUHQACBAO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C21H6Cl6O7/c22-7-1-4(19(30)31)12(25)11-10(7)20(32)34-21(11)5-2-8(23)15(28)13(26)17(5)33-18-6(21)3-9(24)16(29)14(18)27/h1-3,28-29H,(H,30,31)
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| Chemical Name |
2',4,4',5',7,7'-hexachloro-3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO :~125 mg/mL (~214.41 mM; with sonication)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7153 mL | 8.5765 mL | 17.1530 mL | |
| 5 mM | 0.3431 mL | 1.7153 mL | 3.4306 mL | |
| 10 mM | 0.1715 mL | 0.8576 mL | 1.7153 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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