| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 1g |
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| Other Sizes |
|
| Targets |
iNOS Human Endogenous Metabolite
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|---|---|
| ln Vitro |
Chondroitin sulfate sodium (from shark cartilage) is a type of sulfated glycosaminoglycan, a linear polysaccharide composed of repeating disaccharide units composed of uronic acid and N-acetylhexosamine. A variety of pathogens, including parasites, bacteria, and viruses, have been shown to utilize cell surface Chondroitin sulfate chains to attach to and infect host cells [1]. Chondroitin sulfate occurs naturally in the extracellular matrix of connective tissues such as bone, cartilage, skin, ligaments and tendons. Chondroitin sulfate has shown a range of beneficial effects: anti-inflammatory effects, increase in type II collagen and proteoglycans, reduced bone resorption, and a better anabolic/catabolic balance in chondrocytes [2]. A wide range of chondroitin sulfate concentrations (eg, 12.5 to 2000 mg/mL, but usually less than 200 mg/mL) has been used in in vitro studies. Chondroitin suLfate (200 mg/mL) reduces the sensitivity of chondrocytes to single nucleotide polymorphism-induced apoptosis [3]. Chondroitin sulfate can reduce inflammatory mediators and apoptotic processes, and can reduce the protein production of inflammatory cytokines, iNOS, and MMPs [4].
|
| ln Vivo |
The high content of chordoriin sulfate in the aggregated protein plays an important role in enabling cartilage to resist tensile stress under various loading conditions, providing resistance and elasticity to the tissue. Studies have shown that chordoriin sulfate interferes with the progression of structural changes in joint tissue and is used to treat patients with osteoarthritis [3]. Chondroitin sulfate is mainly administered orally at a dose of 800 to 1200 mg/day. Chondroitin sulfate is rapidly absorbed from the gastrointestinal tract. The absorbed chordoriin sulfate reaches the blood compartment in the form of 10% chordoriin sulfate and 90% depolymerized low molecular weight derivatives [5].
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| References |
|
| Exact Mass |
1525.09
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|---|---|
| CAS # |
9082-07-9
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| Appearance |
White to off-white solid powder
|
| Density |
1.94g/cm3
|
| SMILES |
[C@H]1(O[C@@H]([C@@H](O)[C@H](O[C@H]2[C@H](O)[C@H]([C@H](O[C@@H]3O[C@@H]([C@@H](O)[C@H](O[*])[C@H]3NC(=O)C)COS([O-])(=O)=O)[C@@H](C([O-])=O)O2)O)[C@H]1NC(=O)C)COS([O-])(=O)=O)O[C@H]1[C@H](O)[C@H]([C@H](O[C@H]2[C@H](O)[C@H](O[C@@H](O[C@H]3[C@H](O)[C@H]([C@H](O[*])O[C@@H]3C([O-])=O)O)[C@@H]2NC(=O)C)COS([O-])(=O)=O)O[C@@H]1C([O-])=O)O.[Na+].[Na+].[Na+].[Na+].[Na+].[Na+]
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O :~50 mg/mL (with sonication)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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