| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Ceramide 3B does not have a specific pharmacological target in the traditional sense; rather, it is a structural lipid. It is a component of the skin's stratum corneum and plays a crucial role in maintaining the skin barrier function. It interacts with other lipids in the extracellular matrix of the epidermis to form a lamellar structure that prevents water loss.
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| ln Vitro |
In vitro, Ceramide 3B is used as a research tool to study skin barrier function and lipid organization. It can be incorporated into model lipid membranes or skin equivalents to assess its effects on barrier properties and permeability. Its "activity" is its ability to form lamellar structures and enhance barrier function.
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| ln Vivo |
In vivo, Ceramide 3B is used in cosmetic and dermatological formulations to restore skin barrier function. It is applied topically and is known to be a physiological lipid that helps maintain skin hydration and integrity.
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| Enzyme Assay |
In vitro enzyme or receptor binding assay protocols are not applicable to Ceramide 3B, as it is a structural lipid. Instead, its properties are studied using biophysical techniques such as differential scanning calorimetry (DSC), X-ray diffraction, and Fourier-transform infrared spectroscopy (FTIR) to analyze its lipid phase behavior and interactions.
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| Cell Assay |
In vitro cell-based assay protocols for Ceramide 3B typically involve using cultured skin models, such as reconstructed human epidermis (RHE) or keratinocyte cultures. The compound is applied topically to the models, and its effects on barrier function (e.g., transepidermal water loss) and lipid organization are assessed.
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| Animal Protocol |
In vivo animal experimental protocols for Ceramide 3B would typically involve topical application to animal skin (e.g., mice or pigs) to assess its effects on skin barrier function. Skin hydration, barrier recovery after tape stripping, and histological analysis of the stratum corneum are common endpoints.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Ceramide 3B are not relevant in the traditional sense, as it is a topical agent. It is a highly lipophilic molecule that is incorporated into the skin's lipid matrix. Its metabolism and systemic absorption after topical application are minimal.
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| Toxicity/Toxicokinetics |
Ceramide 3B is considered safe for topical use in cosmetic and dermatological applications. It is a physiological lipid and is generally well-tolerated. No significant systemic toxicity is expected from dermal application. It is not intended for oral or injectable use.
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| References | |
| Additional Infomation |
N-Oleoyl Phytosphingosine is a phytoceramide with an N-acyl group of oleoyl (9Z-octadecenoyl). Functionally, it is related to oleic acid. Ceramide NP is a lipid molecule belonging to the ceramide class of lipid molecules. Ceramides are the main lipid components of the stratum corneum of human skin. Ceramide 3 is formed by the N-acylation of a phytosphingosine backbone with a saturated fatty acid (stearic acid). It is widely used as a moisturizer in various cosmetics and personal care products. Ceramide 3 works synergistically with ceramide 1 to enhance the skin barrier function. See also: Ceramide 3 (note moved here).
Ceramide 3B is a research-grade lipid used in skin barrier research and in the formulation of cosmetic and dermatological products. It is a physiological component of the skin's stratum corneum. It has not entered clinical trials as a drug but is used in personal care products. Its mechanism of action is structural, contributing to the formation of the skin's lipid barrier. The compound is available for research and development purposes. |
| Molecular Formula |
C36H71NO4
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|---|---|
| Molecular Weight |
581.95
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| Exact Mass |
581.538
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| CAS # |
178436-06-1
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| PubChem CID |
57378373
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| Appearance |
White to off-white solid powder
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| Density |
0.982 at 20℃
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| Boiling Point |
722℃ at 101.325kPa
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| LogP |
12.4
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
32
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| Heavy Atom Count |
41
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| Complexity |
568
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| Defined Atom Stereocenter Count |
3
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| SMILES |
O[C@@H]([C@H]([C@H](CO)NC(CCCCCCC/C=C\CCCCCCCC)=O)O)CCCCCCCCCCCCCC
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| InChi Key |
ATGQXSBKTQANOH-UWVGARPKSA-N
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| InChi Code |
InChI=1S/C36H71NO4/c1-3-5-7-9-11-13-15-17-18-19-21-23-25-27-29-31-35(40)37-33(32-38)36(41)34(39)30-28-26-24-22-20-16-14-12-10-8-6-4-2/h17-18,33-34,36,38-39,41H,3-16,19-32H2,1-2H3,(H,37,40)/b18-17-/t33-,34+,36-/m0/s1
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| Chemical Name |
(Z)-N-[(2S,3S,4R)-1,3,4-trihydroxyoctadecan-2-yl]octadec-9-enamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Typically soluble in DMSO (e.g. 10 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7184 mL | 8.5918 mL | 17.1836 mL | |
| 5 mM | 0.3437 mL | 1.7184 mL | 3.4367 mL | |
| 10 mM | 0.1718 mL | 0.8592 mL | 1.7184 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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